Global ETD Search
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Showing 1 to 8 of 8 for “"3' end formation"”.
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Functional Analysis of Drosophila Integrator Complex In Snrna 3' End Processing
… and the processing of the replication-dependent histone pre-mRNAs. Formation of the 3’ end of these snRNAs is carried out by a poorly characterized, twelve-membered protein complex named Integrator Complex.</p> <p>In the effort to understand Integrator Complex function in the formation of …
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The functions of the RNA polymerase II CTD in transcription and RNA processing
… and RNA processing- capping, splicing, and 3' end formation. For example, Ser 5, phosphorylated in the start of transcription, promotes the recruitment of capping enzyme, and Ser 2 phosphorylation facilitates RNA 3' end formation and transcription termination by acting as a landing pad for …
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Interrogating Dux4 Mrna 3′ End Processing
… but contributes to FSHD pathology due to Dux4-dependent cellular reprogramming.</p> <p>We aim to understand what regulatory elements facilitate the cleavage and polyadenylation (CPA) of the Dux4 mRNA beyond the non-consensus PAS and to determine if inefficient CPA underlies the poor expression of …
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On co-transcriptional splicing and U6 snRNA biogenesis
… a protein: a cap structure is added to its 5’ end, noncoding, intervening sequences (introns) are removed and coding exons are ligated together and a poly(A) tail is added to its 3’end. Splicing, the process of intron removal, is carried out in the spliceosome, a megacomplex comprehending up to …
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Diverse RNA processing pathways important for post-transcriptional gene regulation
… ensures high fidelity definition of miRNA 5' ends, which determine target specificity. Small RNAs from Caenorhabditis elegans and Drosophila melanogaster are extensively surveyed here using high-throughput sequencing. Analysis of these libraries led to the discovery of a novel miRNA biogenesis …
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The Role of ATP and Cleavage Factor Phosphorylation in pre-mRNA 3' Cleavage Reaction
<p>The 3' end cleavage/polyadenylation (3' processing) is important in generating a functional messenger RNA (mRNA) transcript. It is long-known that ATP can significantly stimulate the in vitro cleavage of adenovirus type 2 L3 (Ad2L3) RNA substrate. Here, we used ATP analogs in structure-activity …
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Novel RNA Targets of the Spinal Muscular Atrophy Protein
… to pre-mRNA splicing, including histone mRNA 3' end formation and mRNA decay. Interestingly, in addition to its direct binding to Sm proteins, SMN has been shown to associate in vitro with members of the LSm family as well as other RNA binding proteins, implicating the SMN complex in the biology …
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Structure-Function Analysis of Human Integrator Subunit-4
… and histone mRNA expression. Although the 3′ end formation mechanism and processing machinery of other RNAPII transcripts such as mRNA has been well studied, the mechanism of snRNA 3′ end processing has remained a mystery until the recent discovery of the machinery that mediates this process. …