Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"2DE"”.
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Understanding adaptive regulation of skeletal muscle physiology in the Weddell seal: a proteomics approach
… using two dimensional gel electrophoresis (2DE), quantified, and identified. This study validated the use of cross-species analysis, which was of paramount importance due to the fact that the pinniped genome is largely unidentified, and established a 2DE protocol tailored to suit the unique …
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The mitochondrial protein profile changes during the aging process
… extrahiert. Großgel 2D-Elektrophorese (2DE) und Westernblots wurden für die Analyse der Proteine verwendet. Die Expression von zwei Untereinheiten des Komplexes I und einer Untereinheit des Komplexes IV der Atmungskette nahm mit zunehmendem Alter ab. Eine Untereinheit von Komplex III, …
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Development of Methods for the Study of Phosphoproteins
… done using two dimensional gel electrophoresis (2DE) separation, visualization of phosphoproteins by radiolabeling or a phosphoprotein specific dye, followed by MALDI-TOF identification. Because radiolabeling of phosphoproteins is very sensitive and straightforward to quantify, such analysis can …
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Characterization of a novel aggregate-gland-derived spider silk protein in Latrodectus Hesperus : from sequence to propsoed function
… analyzed by two-dimensional gel electrophoresis (2DE) and MS/MS analysis. AgSFl was localized in the web scaffolding junctions and our data supports a role as an adhesive silk protein that serves as a center for connecting scaffolding fibers that functions to reduce the tensile strength of …
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Proteomic analysis of cellular models of neurodegeneration and mitochondrial dysfunction
… an optimised 2-dimensional gel electrophoresis (2DE) protocol and the reproducibility of the method was investigated. A preliminary study comparing the mitochondrial proteome profile from two different states of mouse N2a neuroblastoma cells, mitotic and differentiated, was undertaken to …
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The proteomic analysis of resistance to anticancer therapy in human breast cancer
… optimise two-dimensional gel electrophoresis (2DE) and matrix-assisted laser desorption/ ionisation time-of-flight mass spectrometry (MALDI-TOF MS) and to exploit this global proteomics approach to identify molecular markers associated with resistance to anticancer therapy in human breast …
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Durch Sirolimus induzierte frühe Veränderungen des Proteoms und Phosphoproteoms in humanen T-Lymphoblasten
… Proteine und phosphorylierte Proteine in 2DE-Gelen unterscheiden und mittels einem Q-TOF Ultima Global Massenspektrometer identifizieren zu können. Ergebnisse und Diskussion: Die Inkubation mit 2,5 µmol/l SRL führte zu einer annähernd 70%igen Inhibition der Zellproliferation. Die für 30 …
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Patterns of protein expression in tissues of the killifish, Fundulus heteroclitus and Fundulus grandis
… xi Chapter 4 focuses on the use of advanced 2DE-MS/MS to characterize the proteome of multiple tissues in F. grandis. Database searching resulted in the identification of 253 non-redundant proteins in five tissues: liver, muscle, brain, gill, and heart. Identifications include enzymes of …
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Analysis of the nuclear proteome of the resurrection plant Xerophyta viscosa (Baker) and its response to dehydration stress
… quantitation (iTRAQ) technologies. Using the 2DE approach, a total of 438 proteins spots were reproducibly detected and analysed of which 18 protein spots were shown to be up-regulated in response to dehydration. These proteins contained both regulatory and functional proteins. The largest …
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Der chirurgische Blick auf die Mitralklappe : Nutzen und Limitation der 3D Echokardiographie am Beispiel des Mitralklappenprolaps
… reliability and rapidity than the conventional 2DE approach. Despite a lower spatial resolution in comparison to conventional 2D echocardiography 3DE provide accurate quantification of prolapsing leaflet segments in a projected surgical view that can be of value in planning surgical mitral valve …
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Ricerca, mediante tecniche di proteomica, di biomarcatori proteici utili nella valutazione della qualità degli alimenti di origine animale
… for the quality of the final product through 2DE methodologies. In the seafood field these techniques are currently used with a discreet success only for vertebrates (fishes), while in the study of the invertebrates (molluscs) there are a lot of difficulties. The results obtained in this work …
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Patterning the Mud Snail Ilyanassa obsoleta: The Role of Cell Signaling and Asymmetric Protein Localization
The polar lobe of Ilyanassa is asymmetrically partitioned into the D lineage of cells. Two of these cells, 3D and 4d, induce proper axial cell fate patterning in the embryo. Based on known embryological data in Ilyanassa, I hypothesized that Notch signaling would be required for this induction. I …
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Evaluación toxicológica de cilindrospermopsina mediante modelos experimentales in vivo e in vitro: daños producidos por estrés oxidativo y potencial efectividad de n-acetilcisteína para contrarrestarlos
… Se emplearon geles de dos dimensiones (2DE) para el análisis proteómico de hojas de tomate. Además, se comprobó que la toxina puede ser el principal factor responsable del estrés fisiológico observado en las plantas, aunque existen otras moléculas bioactivas sintetizadas por las células …