Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"2D-PAGE"”.
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Proteomic identification of putative biomarkers of neo-adjuvant chemotherapy resistance in luminal (ER+) breast cancer
… approaches (antibody microarray/AbMA and 2D-PAGE with MALDI-TOF/TOF MS) to investigate a pilot series of breast cancer samples our research group recently identified 14-3-3 theta/tau, tBID and BcL-XL as putative biomarkers of response to neoadjuvant chemotherapy (Hodgkinson et al J Prot …
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The identification of biomarkers of chemotherapy resistance in breast cancer using comparative proteomics
… polyacrylamide gel electrophoresis (2D-PAGE) with MALDI-TOF/TOF mass spectrometry and antibody microarray analysis. DEPs were submitted to Ingenuity Pathway Analysis (IPA) to identify any canonical pathway links, confirmed using western blotting and clinically validated in a pilot …
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UV-B-Induzierte Genexpression bei der Buche Fagus sylvatica L.
… für die Daten in der vorliegenden Arbeit. Die 2D-PAGE der löslichen Gesamtproteine und in vitro translatierten Proteine wurde stets zweifach durchgeführt und jeweils die Gele mit der besten Auflösung als Einzelbestimmung ausgewertet. Die Untersuchungen auf Ebene des löslichen Gesamtproteins der …
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Characterization of metronidazole resistant Helicobacter pylori
… peroxidase (TP) expression as shown by 2D-PAGE. Furthermore, these MtzR- Hp were non-motile and showed the lack of flagella. It is therefore hypothesized that the different expression of various TP isoforms in the presence of sub-lethal metronidazole concentration may have a role in …
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The proteomic analysis of B-cell receptor signaling in chronic lymphocytic leukaemia
… polyacrylamide gel electrophoresis (2D-PAGE). The gels were stained with coomassie blue total protein stain and analysed with statistical software. Proteins with a two-fold (p less than 0.05) change in expression between stimulated and unstimulated samples were excised from the gels …
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Analisi Molecolare del Melanoma
… (FOM 78), by two-dimensional electrophoresis (2D-PAGE) and liquid chromatography¿tandem mass spectrometry (LC-MS/MS). Our comparative proteomic analysis revealed that six proteins were overexpressed in both melanoma cell lines as compared with melanocytes: galectin-1, inosine-5'-monophosphate …
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Oxidative, inflammatory and vascular factors in Alzheimer's disease
… detergent fractionation of brain tissue, 1D and 2D PAGE, western blotting with chemiluminescence detection, ELISA assays of Abeta 1-40 and 1-42, quantitative ECNI GCMS of o- and m-tyrosine as well as metabolites of the kynurenine pathway, quantitative MALDI-TOF assay of hemorphins and LCMSMS …
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Quantifizierung von GAP-43 und die postnatale Entwicklung der molekularen Komplexität im auditorischen Hirnstamm der Ratte
… Mittels der zweidimensionalen Elektrophorese (2D-PAGE) von Gewebeproben des Rattenhirns, die Proteine nach ihrem isoelektrischen Punkt und ihrem Molekulargewicht auftrennt, konnten diese gefunden werden. Dafür wurden der Colliculus inferior (IC) als Teil der auf- und absteigenden Hörbahn und, …
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Chronic low-level Pb exposure during development alters proteins involved in energy metabolism in auditory neurons of the brainstem
… (isolation and separation of proteins by 2D-PAGE; analysis by MALDI-MS) revealed that chronic Pb exposure alters the expression of proteins involved in the regulation of cellular energy metabolism including VDAC and creatine kinase B. Immunohistochemistry confirms that Pb exposure results …
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Chronic low-level Pb exposure during development alters proteins involved in energy metabolism in auditory neurons of the brainstem
… (isolation and separation of proteins by 2D-PAGE; analysis by MALDI-MS) revealed that chronic Pb exposure alters the expression of proteins involved in the regulation of cellular energy metabolism including VDAC and creatine kinase B. Immunohistochemistry confirms that Pb exposure results …
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A Global and Targeted Proteomic Investigation of Aspergillus fumigatus
… of proteins typically under-represented in 2D-PAGE proteome maps, including proteins with multiple transmembrane regions, hydrophobic proteins and proteins with extremes of molecular mass and pI. Pre-fractionation of complex protein samples, by gel-filtration or gold nanoparticle …
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Análise da influência de diferentes sistemas de cultivo sobre o crescimento e as proteínas do sistema digestório de Oreochromis niloticus, L.
… Para os perfis proteômicos foram feitos géis 2D-PAGE utilizando fitas de 13 cm e pH na faixa de 3-10. Pela análise do crescimento zootécnico, apenas os parâmetros de peso médio final, conversão alimentar e sobrevivência foram estatisticamente diferentes. Quanto às atividades enzimáticas, essas …
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Studies on the effects of sub-minimal inhibitory concentrations of antibiotics on the virulence factors of biofilm bacteria
… from results obtained from MIC experiments). SDS-PAGE and 2D-PAGE analysis indicate alteration of outer membrane proteins (OMPs) within the envelope of the pathogen in sub-MIC antibiotic treated samples. The observed rearrangement of lipopolysaccharide (LPS; as observed in LPS gel experiments) may …
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Identification of novel branch points reveals insights into RNA processing
… (including lariats) from linear RNAs using a 2D PAGE gel. This was followed by a custom library preparation protocol that produced insert ends that identified the BP and 5'SS of individual lariats. Using this method, we located known BPs and discovered a substantial number of novel BPs both in …
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Veränderungen in Proteom und Metabolismus bei dilatativer Kardiomyopathie Cathepsin L defizienter Mäuse
ZusammenfassungAusgehend von den beschriebenen morphologischen und funktionellen Aspekten derdilatativen Kardiomyopathie bei Cathepsin L defizienten Mäusen konnten in dieserArbeit weiterführende Erkenntnisse über die Erkrankung gefunden werden. CTSLdefiziente Mäuse zeigen mit durchschnittlich mit …
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The effect of subinhibitory concentrations of antibiotics on virulence factors of <i>Staphylococcus aureus</i> biofilms
… any modulation in its activity.<br/><br/>SDS-PAGE and 2D-PAGE of biofilm and eluate cells in addition to eluate supernatants exhibited remarkable differences in protein profiles among challenged and control biofilms. Such protein shifts would imply in part, alterations in biofilm …
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Identification of Chlamydial Iron-Responsive Proteins during Intracellular Growth.
<p><em>Chlamydia trachomatis</em> is an obligate intracellular bacterium and the most prevalent cause of bacterial sexually transmitted disease. Genital chlamydial infections, marked by chronic, intense inflammation, can lead to genital tissue scarring and infertility and is a contributing factor …
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Studies on T-OMP and the development of antimicrobial tolerance in <i>Pseudomonas aeruginosa</i> PAO1
… sulphate polyacrylamide gel electrophoresis (SDSPAGE) analysis of the OMP profiles from the tolerant-induced cultures illustrated the loss of T-OMP in all cases. Analysis of the sensitive and resistant cultures using twodimensional polyacrylamide gel electrophoresis (2D-PAGE) indicated that the …
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Gene and Protein Profiling of the Preeclamptic Placenta
Aims State-of-the-art methodology was used to screen and profile the placenta, gene and protein expression, for changes related to preeclampsia (PE) and cases with increased resistance in the uterine arteries. Women with increased resistance in the uterine arteries have increased risk of developing …