Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 27 for “"2D-IR"”.
-
Ultrafast 2D IR spectroscopy of membrane peptide systems
… between the intercellular and extracellular environment, playing a critical role in numerous biological processes. Recent research has challenged the long-standing "fluid mosaic model," representing membranes as densely packed, heterogeneous environments. Within these complex membranes are …
-
Studies of biofluid analysis using 2D-IR spectroscopy
… the application of two-dimensional infrared (2D-IR) spectroscopy is evaluated to provide a quantitative analysis of the protein content of blood serum. The foremost challenge is obtaining protein measurements in physiologically relevant solvents as the most informative infrared peak relating …
-
Temperature-jump 2D IR spectroscopy to study protein conformational dynamics
… (T-jump) two-dimensional infrared spectroscopy (2D IR) is developed, characterized, and applied to the study of protein folding and association. In solution, protein conformational changes span a wide range of timescale from nanoseconds to minutes. Ultrafast 2D IR spectroscopy measures …
-
2D IR spectroscopy and computational modeling : application to protein folding and binding
… insight into protein folding and binding is indirectly obtained through kinetics experiments that measure reaction rates and reveal how fast populations of stable states interconvert. Two-dimensional infrared spectroscopy (2D IR) is the central tool developed in this thesis for protein dynamics …
-
Remaining Unperturbed by the Vibrational Response: Probing Vibrational Coupling, Relaxation, and Solvent Effects with 2D IR Spectroscopy
Understanding molecular function first requires an understanding of their native structuresand fluctuations of their environments. One of the most powerful ways to probe the dynamic behavior of molecular systems in solution is through the use of Two-Dimensional Infrared (2D IR) spectroscopy. 2D IR …
-
Ultrafast infrared spectroscopy investigation of DNA-ligand interactions
Two-dimensional infrared spectroscopy (2D-IR) is a label-free analysis method that can provide structural insight into biological processes by uncovering the coupling between the intrinsic, vibrational modes of a molecule. Technological advancements in the last decade have drastically reduced …
-
Two-dimensional infrared spectroscopy of nucleic acids : application to tautomerism and DNA aptamer unfolding dynamics
… of nucleic acids are intimately related to their biological functions; however, our ability to study these molecular dynamics has been largely impeded by the lack of techniques that possess both high time resolution and structural sensitivity. The motivation for the work in this thesis was to …
-
A tale of coupled vibrations in solution told by coherent two-dimensional infrared spectroscopy
Coherent two-dimensional infrared (2D IR) spectroscopy is used as a tool for investigating the molecular structure and dynamics of coupled vibrations in solution on a picosecond timescale. The strongly coupled asymmetric and symmetric carbonyl stretches of Rh(CO)₂C₅H₇0₂ (RDC) dissolved in hexane …
-
Thermal unfolding dynamics of proteins probed by nonlinear infrared spectroscopy
… The spectroscopic tool is nonlinear infrared (IR) spectroscopy of the protein amide I band. Among various nonlinear IR techniques, two-dimensional infrared (2D IR) spectroscopy, which is an IR analogue of 2D NMR, is the most informative. A 2D IR spectrum is obtained from a double Fourier …
-
Multi-mode two-dimensional infrared spectroscopy of peptides and proteins
… through multi-mode two-dimensional infrared (2D IR) spectroscopy is developed. The experimental framework for generation of broadband infrared lasers and robust new approaches to 2D IR spectroscopy are demonstrated. Long-term phase stability is achieved through the development of a passively …
-
Insight into the Local Solvent Environment of Biologically Relevant Iron-nitroysl Systems through Two-Dimensional Infrared Spectroscopy
Iron-nitrosyl systems, particularly in the form of heme proteins, with their iron metal active sites play an important role in biological systems. Heme proteins act as storage, transporters, and receptors for nitric oxide (NO), a signaling molecule that is important in immune, nervous, and …
-
Observing the unfolding transition of [beta]-hairpin peptides with nonlinear infrared spectroscopy
… physical interactions. In this thesis, P-hairpin peptides are used as model systems for studying P-sheet secondary structure. Hairpin folding has been studied for a number of years, but there is still debate in the literature about the relative importance of the cross-strand hydrogen bonds, …
-
Computational and Theoretical Modeling of Two Dimensional Infrared Spectra of Peptides and Proteins
Two-dimensional infrared (2D-IR) spectroscopy evolved out of the theoretical underpinnings of nonlinear methods to provide a means of investigating detailed molecular structure on ultrafast timescales. This opens up new possibilities for the study of protein folding and molecule-solvent …
-
Coherent two-dimensional infrared spectroscopy : a study of coupled vibrations
… experimental techniques used in two-dimensional (2D) infrared (IR) spectroscopy, outlines how third-order nonlinear response of a multi-level vibrational system is calculated, and provides a detailed methodology of line shape analysis in 2D spectroscopy. Specific emphasis is given to inherent …
-
Hydrogen bond rearrangements and the motion of charge defects in water viewed using multidimensional ultrafast infrared spectroscopy
… two-dimensional infrared spectroscopy (2D IR), applied to the O-H stretching transition of a dilute solution of HOD in NaOD/D20. The frequency of the O-H stretch, (OH, is highly sensitive to the configuration of its hydrogen bonding partner. 2D IR spectroscopy allows us to measure rapid …
-
Stapling and Unstapling Peptides and Proteins With S-Tetrazine
… two-dimensional infrared spec-troscopy (2D IR). The s-tetrazine molecule possesses the photochemical properties of an ideal phototrigger, as such, s-tetrazine was employed towards the development of tech-niques capable of capturing the fastest structural transitions of biomolecules with …
-
Probing Solvation Shell Dynamics of Organometallic Complexes via Two-Dimensional Infrared Spectroscopy
… of Fourier transform infrared spectroscopy (FTIR) and two dimensional infrared spectroscopy (2D-IR) to organometallic complexes in various solvents. FTIR spectroscopy shows the fundamental vibrational modes of a complex, yielding a spectrum that acts as a molecular fingerprint that allows for …
-
Commande de robots industriels par systèmes de vision
… mieux répondre aux besoins des industries partenaires au projet et de s’aligner avec les technologies de l’industrie 4.0. Dans l’optique d’atteindre cet objectif, le travail vise à relever plusieurs défis rencontrés avec la commande des robots manipulateurs dans un contexte de fabrication …
-
Three-dimensional T1 quantification techniques for assessment of cartilage quality using dGEMRIC
… standard two-dimensional inversion recovery (2D-IR). A 3D variable flip angle (3D-VFA) T1 quantification sequence in combination with a B1 mapping sequence was also investigated. The results with and without B1 correction was studied in vivo and in phantoms. It was concluded that 3D-VFA should …
-
Observing residual structure in disordered peptides with multidimensional infrared spectroscopy
… of NMR or ESR, little is known about their structure or function. By combining isotope-edited two-dimensional infrared spectroscopy (2D IR) with spectral modeling based on molecular dynamics simulations, this work will show that one can measure the residual structure and conformational …
Page 1 of 2