Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"2D-DIGE"”.
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Use of comparative proteomics to study a novel osteogenic nanotopography
… fluorescence difference gel electrophoresis (2D DIGE) is a new method for assessing protein expression strategies and here, a micro-grooved topography was used as a model for protocol optimization. The protocol was successfully developed and proved that 2D DIGE can be used as a powerful tool …
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Regulation of the carbon-concentrating mechanism in Chlamydomonas reinhardtii
… difference in gel electrophoresis (2D-DIGE) was used to compare the soluble proteome of one low CO2-adapted pyrenoid-less RBCS mutant compared to the pyrenoid-positive wild-type. This analysis identified only a few differentially expressed proteins, none of which were directly …
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Telomerase a prognostic marker an therapeutic target
… A comprehensive proteomic study utilizing 2D-DIGE and MALDI-TOF were used to assess the effect of inhibiting two different regulatory mechanisms of telomerase in glioma. RNAi was used to target hTERT and Hsp90α. Inhibition of telomerase activity resulted in down regulation of various …
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Etude protéomique de l'incidence d'une hypertriglycéridémie sur la mitochondrie de foie de souris
… caractérisé, par une analyse protéomique en 2D-DIGE, les adaptations du métabolisme mitochondrial hépatique à une hypertriglycéridémie modérée et sévère chez la souris HuApoC-III afin d’établir la dépendance de la réponse métabolique vis-à-vis du niveau d’hypertriglycéridémie. Cette analyse a …
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Rheumatoide Arthritis: Proteomische Analyse von Serum und synovialen Fibroblasten zur Detektion von Biomarkern
… kultiviert und die Lysate dieser Zellen mittels 2D-Gelelektrophorese analysiert. Es konnten spezifische Vimentinfragmente detektiert werden, die nur bei RA-Patienten vorkamen. Vimentin steht hierbei im Verdacht, die Verfügbarkeit vom p53 zu beeinflussen und könnte somit die Apoptoseresistenz …
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Applications of Mass Spectrometry to Analysis of Prodiginines, Bioactivated Methylenedianiline Intermediates, and Hypoxia Induced Changes in the Zebrafish Skeletal Muscle Proteome
… difference in-gel electrophoresis (2D-DIGE) in vivo after 48 h in hypoxia vs. normoxia. The results showed that proteins involved in mitochondrial oxidative metabolism are down-regulated, whereas glycolytic enzymes are up-regulated to compensate for the loss of ATP synthesis in …
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Investigating biomarkers in clinical samples that are predictive of response in multiple myeloma
… enrich for low-abundance proteins, followed by 2D-DIGE separation and subsequent mass spectrometry to identify differentially expressed protein spots. ELISA-based assays were used to validate the candidate protein biomarkers using unfractionated serum samples from 51 consecutive newly-diagnosed …
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The genetic and biochemical analysis of Drosophila Wwox protein function.
… molecular functions of the Wwox protein. 2D-DIGE analysis was conducted on a number of different fly genotypes expressing differing levels of Wwox protein in both adult and embryonic flies. The proteomic changes resulting from lack of Wwox function as well as Wwox over-expression were …
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Investigation of the intrinsic mechanism of drug resistance in multiple myeloma
… myeloma resistance to bortezomib treatment using 2D-DIGE analysis. Focussing on bortezomib resistance models, we found that that overexpression of P-gp attenuates bortezomib activity. Bortezomib is a P-gp substrate and a combination of P-gp inhibitor and bortezomib is able to overcome resistance. …
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Proteomic studies of the pheromone biosynthetic pathway in the bark beetle, Ips pini, and molecular modeling of an insect-specific aldehyde oxidative decarbonylase, CYP4G2
… to examine protein levels in midgut tissue. 2D-DIGE and ProQ Diamond phosphoprotein staining were used to examine the expression of proteins and phosphorylated proteins involved in the pheromone producing midgut tissue. The results show a lack of a linear relationship between the high …
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Characterisation of Streptococcus pneumoniae opacity phase variation
… out to comprehensively characterise phenomic (2D-DIGE), transcriptomic (DNA microarray) and genomic (IonTorrent sequencing) differences between opacity variants in three unrelated pneumococcal strains (D39, serotype 2; WCH16, serotype 6A; and WCH43, serotype 4). Proteomic, transcriptomic and …
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Investigation of the molecular mechanisms underlying the invasive phenotype in a panel of lung cancer cell lines
… effect of mir-29a was not cell line specific. 2D-DIGE proteomic profiling of cells transfected with mir-29a generated a list of differentially regulated protein spots, some of which were identified by MALDI-TOF and LC-MS analysis. Bioinformatic analysis revealed that the majority of these …
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Interactions between bifidobacteria and other human intestinal microbial populations
… cadena corta (AGCC) a partir de carbohidratos no digeribles de la dieta, y protege frente a patógenos. Tres de los géneros más representativos de la microbiota son Bacteroides, Faecalibacterium y Bifidobacterium. Las interacciones entre estos microrganismos, así como con los compuestos presentes …
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Identification of the metabolites responsible for colour stability from different lamb sires and elucidation of the underlying biochemical pathways : A thesis submitted in partial fulfilment of the requirements for the Degree of Doctor of Philosophy in Biochemistry at Lincoln University
… In proteomics, the combination of gel-based 2D-DIGE and mass spectrometry identified pyruvate kinase isozymes M1/M2 isoform 4, aldehyde dehydrogenase (mitochondrial) and ATP synthase subunit d; whereas the label-free proteomics discovered dihydrolipoamide S-succinyltransferase, …