Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"2D electrophoresis"”.
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Proteomics approach to investigate separation behavior of proteins in ion-exchange chromatography
… hydrophobic partitioning with two-dimensional (2D) electrophoresis. Protein pI and MW were obtained from 2D gels directly using the known pI gradients and the positions of MW standards. Protein partition coefficients were obtained by quantifying protein spot mass for the top and bottom phase …
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The role of soluble factors affecting the major histocompatibility complex class I molecules In an IN VITRO model of the fetomaternal interface
… Also, PIF modified protein profile, detected by 2D electrophoresis. Compared with the untreated cells, 14 proteins were over-expressed and 8 were under-expressed. Our study suggests that PIF, has a regulatory effect on HLA-I in this cellular model and the fact that not only HLA-G was …
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Activation of HGF/Met Signaling Induces Delayed STAT3 Phosphorylation
… was checked by Western blot analysis. RT-PCR, 2D electrophoresis and MALDI-TOF analysis was performed to identify possible mediator(s). We found the tyrosine phosphorylation of STAT3 from 2 hours after the activation of HGF-Met signaling. The delayed phosphorylation of STAT3 was blocked by …
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The characterisation and identification of body fluid proteins for forensic purposes.
… and the expressed proteins are compared by 2D electrophoresis. Human body fluids differ in function, composition and protein expression. Proteomics therefore seemed an ideal application to isolate the proteins that are characteristic of and specific to, different fluid types. Both saliva and …
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Quantifizierung von GAP-43 und die postnatale Entwicklung der molekularen Komplexität im auditorischen Hirnstamm der Ratte
… Mittels der zweidimensionalen Elektrophorese (2D-PAGE) von Gewebeproben des Rattenhirns, die Proteine nach ihrem isoelektrischen Punkt und ihrem Molekulargewicht auftrennt, konnten diese gefunden werden. Dafür wurden der Colliculus inferior (IC) als Teil der auf- und absteigenden Hörbahn und, …
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Biomarkers identification in fibromyalgia syndrome
… was performed by combining two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) and serum purine levels were quantified using reverse phase high performance liquid chromatographic (RP-HPLC). In our study, using the proteomic approach, we have identified differentially expressed …
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NUTRITIONAL QUALITY AND FOOD SAFETY OF FOOD SOURCES AND FOOD PROCESSING BY-PRODUCTS
… classical analysis methods, such as SDS-PAGE and 2D-electrophoresis, with chromatographic purification and mass spectrometry analysis by comparing the results with proteomic data of domesticated species.