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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 50 for “"26S proteasome"”.
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Substrate recognition by the 26S proteasome
The 26S proteasome is a large protein complex found in all eukaryotes. It controls the degradation of a wide range of proteins in the cell, and thus it is crucial for homeostasis, regulated cell division and apoptosis. It is known that specific signals (called degrons) on the substrates are …
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A Role for PI31-Mediated Proteasome Regulation in Proteostasis and Neuronal Health
… of proteins is achieved by the Ubiquitin-Proteasome System (UPS), which governs a variety of cellular processes such as apoptosis, cell cycle progression, protein quality control, and metabolism. Using this system, cells maintain homeostasis by quickly and irreversibly altering signaling …
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REGULATORY MECHANISMS OF TRANSCRIPTION AND ASSOCIATED DNA REPAIR
… view of the process. Over the recent years, 26S proteasome has been implicated in transcriptional regulation through its proteolytic and non-proteolytic activities. While, the proteolytic role of proteasome in transcription has been extensively studied, its non-proteolytic function is poorly …
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Probing Proteasome Inhibition By Metal Copmplexes As A New Route For Anticancer Therapy
… tested against the inhibition activity of the 26S proteasome. Selected metal ions ranging from transition to main group elements have been incorporated in various ligand systems containing phenolate and pyridyl donor sets. The mechanistic behavior of these complexes in solution has been …
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Signal Specific Ubiquitination and Degradation of IkBa
… IkB is rapidly ubiquitinated and targeted for 26S proteasome mediated degradation, thus liberating NF-kB for transport to its nuclear destination. The current project was initiated to reconstitute this pathway in vitro by using the purified ubiquitination and degradation machinery to degrade …
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Transcriptional Regulatory Mechanisms of Ribosomal Protein Genes
… microarray analysis has implicated the role of 26S proteasome in transcriptional regulation of the ribosomal protein genes tying protein degradation to protein synthesis pathway. To determine the mechanism as to how the 26S proteasome promotes transcription of the ribosomal protein genes a …
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Part 1: Isolation of Orexin Receptor Regulators via a Microarray-Based,Two-Color Cell Binding Screen. Part II: Targeted Inactivation of Proteins triggered by Visible Light.
… small molecule ligands targeting VEGFR2 or the 26S proteasome. When irradiated with visible light, these reagents showed significantly increased potencies in inhibiting VEGF-induced VEGFR2 activation or proteolytic activity of the 26S proteasome.
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SCF SKP2B - AND KPC1-DEPENDENT DEGRADATION OF CYCLIN-DEPENDENT KINASE INHIBITOR KRP1 AND CELL CYCLE REGULATION IN ARABIDOPSIS THALIANA
… in planta and its degradation depends on the 26S proteasome. Further, an SCF complex composed of CUL1 and SKP2b regulates KRP1 degradation. These results suggest that SCF SKP2B targets KRP1 for degradation by the 26S proteasome to regulate the G1-S transition of the cell cycle. In addition to …
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Proteomic identification of putative biomarkers of radiotherapy resistance
… pathway. The differential expression of both the 26S Proteasome and DR4 were confirmed by western blotting. Clinical assessment using immunohistochemistry revealed a significant association between expression of the 26S Proteasome and radioresistance in breast cancer.DiscussionA large number of …
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Detection of Polypeptide Interactions Via Periodate Triggered Dopa Crosslinking
… to map peptide-protein interactions between the 26S proteasome and activation domains as well as the Arp 2/3 complex and the CA peptide. Finally, we present the creation of a chimeric molecule consisting of the biarsencial fluorescent reporter FLAsH conjugated to 3,4-Dihydroxyphenylalanine as a …
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Coordinated of Post-Translational Modifications of Yeast Transcriptional Activator Gcn4
… its ubiquitination and subsequent proteolysis by 26S proteasome. Previous studies showed that Gcn4 becomes sumoylated at two Lys residues (K50, 58), specifically after binding to target gene promoters. However, it is not clear how promoter-associated sumoylation of Gcn4 is coordinated with its …
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Modulation of Transcription Factor Activity by Mono-Ubiquitin
The Ubiquitin-Proteasome Pathway plays both proteolytic and non-proteolytic roles in the regulation of transcription. We recently reported that the ATPases of the 26S proteasome can destabilize activator-DNA complexes in a non-proteolytic manner that requires direct interactions between the Rpt4 …
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Structural and biophysical analysis of the proteasomal deubiquitinase, UCH37
… is a deubiquitinating enzyme associated with the 26S proteasome, the primary protein degradation machinery in eukaryotic cells. UCH37 is responsible for the disassembly of polymeric ubiquitin chains, or polyubiquitin, which have been ligated onto proteins in order to target them for degradation. …
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Characterizing The Role of Uchl5 In Metastatic Melanoma
… a deubiquitinating enzyme and interacts with the 26S proteasome complex and the INO80 chromatin remodeling complex. While UCHL5 has been shown to be overexpressed in many cancers, it has not been well characterized in melanoma. We investigated the role of UCHL5 in metastatic melanoma <em>in …
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Characterization of the Arabidopsis thaliana Auxin F-Box Family Members AFB4 and AFB5
… protein is to be targeted for degradation by the 26S proteasome. This ubiquitin-mediated protein degradation system is highly conserved throughout eukaryotic cells, including the model plant Arabidopsis thaliana. In Arabidopsis many growth and developmental process are influenced by the plant …
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Exploring the link between RAG1 Cellular Distribution and Components of the CRL4DCAF1 E3 Ligase
… the CRL4DCAF1 E3 ubiquitin ligase, and the 26S proteasome. RAG1 sequestration in nucleoli and nuclear puncta have been implicated in regulating its activity. RAG1 nucleolar egress and puncta formation are dependent on the same domain as DCAF1 association. We investigated whether DCAF1 or the …
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Characterization of the IBR5-PAD1 Interaction in Arabidopsis Auxin Response
Many plant hormones utilize the ubiquitin-proteasome system (UPS) to modulate the expression of specific genes involved in various developmental processes as well as responses to environmental stress. In this process the target proteins are polyubiquitinated by a multi-subunit E3 ubiquitin ligase …
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Role of communication between subunits and enzymes in ClpXP-mediated substrate unfolding and degradation
… and functional similarity to the eukaryotic 26S proteasome. ClpXP consists of hexameric ClpX rings stacked coaxially against the double-ring ClpP₁₄ peptidase. ClpXP's peptidase active sites reside in a sequestered chamber accessible through a narrow channel which excludes native, folded …
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An investigation into the role of the ubiquitin-proteasome system in plant defence
… to the other organisms. In plants, the ubiquitin-26S proteasome pathway appears to be particularly involved in regulating plant growth, development and defence signalling. This project is concerned with establishing potential involvement of UBP12 and UBP13 in plant defence. The …
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Frustration of protein folding from in vitro to in vivo
… 5 reports work on protein recognition by the proteasome, where we investigated protein waste recycling in cells and found that protein re-folding is a vital process in 26S proteasome to initiate protein degradation .
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