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Showing 1 to 20 of 61 for “"23S"”.
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The Structure and Evolution of The Prokaryotic 23s Ribosomal Ribonucleic Acid
Made available in DSpace on 2014-12-14T07:14:48Z (GMT). No. of bitstreams: 1 7913621.pdf: 4198618 bytes, checksum: 48053060e4eed5ce5512a7543ceaa279 (MD5) Previous issue date: 1978
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Degradation of 23S rRNA in Azithromycin-Treated Ribonuclease Mutants of <em>Escherichia coli</em>.
… synthesis rates were measured. Degradation of 23S rRNA was examined by hybridization with a 23S specific probe. Ribonuclease II and polynucleotide phosphorylase mutants demonstrated hypersensitivity to the antibiotic and showed a greater extent of 23S rRNA accumulation, suggesting that these …
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Novel Ribosome Biogenesis in the Lyme Disease Spirochete Borrelia burgdorferi
… most bacteria, the three rRNA transcripts (16S, 23S, and 5S rRNAs) that form the ribosome are produced as a single transcript from an operon with minimal spacing between genes. In the B. burgdorferi genome, however, a single 16S rRNA gene is encoded more than 3 kb from the bicistronic 23S-5S rRNA …
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Novel Ribosome Biogenesis in the Lyme Disease Spirochete Borrelia burgdorferi
… most bacteria, the three rRNA transcripts (16S, 23S, and 5S rRNAs) that form the ribosome are produced as a single transcript from an operon with minimal spacing between genes. In the B. burgdorferi genome, however, a single 16S rRNA gene is encoded more than 3 kb from the bicistronic 23S-5S rRNA …
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Investigation of a ribosomal RNA operon of Frankia sp. strain AcN14a.
… actinomycete rRNA sequences. The mature 16S, 23S and 5S rRNAs are predicted to contain 1509, 3105 and 120 nucleotides, repectively. Thus, Frankia 16S rRNA is smaller than that of E. coli whereas the 23S rRNA of Frankia is longer than it E. coli counterpart. In spite of these size differences, …
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Molekulare Identifizierung von Neisseriaceae und Moraxellaceae mittels ribosomaler DNA-Sequenzierung
… partieller Abschnitte der 16S- oder 23S-rDNA können Bakterien meist exakt spezifiziert werden. Hauptziel der vorliegenden Arbeit war es, hypervariable rDNA Abschnitte zu finden, die von stark konservierten Regionen flankiert werden, um auf molekularer Ebene Mitglieder der Familie …
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Caracterização e análise filogenética molecular de novos isolados fixadores de nitrogênio
… dos genes para os RNAs ribossomais de 16S e 23S (região intergênica 16S-23S rDNA) foi realizada no presente trabalho a partir das técnicas de Polimorfismo de Fragmentos de Comprimento Aleatório amplificados (PCR-RFLP), aplicada ao 16S rDNA e 16S-23S rDNA e sequenciamento de DNA, aplicada ao …
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Comparative Serological and Molecular Analysis of Group VIII <em>Spiroplasma</em> from Australia and North America
… was to investigate the utility of the 16S-23S rDNA intergenic spacer region sequence as a means to distinguish these closely related strains.</p> <p>We chose Group VIII strains for this analysis because they were not separated by 16S rDNA analysis. We generated 16S-23S rDNA intergenic …
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Evolution and Engineering of RNA-based Macromolecular Machines
… mass of the ribosome, in the form of the 16S, 23S and 5S ribosomal RNAs (rRNAs). Despite the importance of the bacterial ribosome in biotechnological applications and as a target for antibiotics, most rRNA mutations have not been characterised for their effects on translation. In Chapter 4, I …
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Aufklärung der Funktion von zwei rRNA-Methyltransferasen und Untersuchungen zur Eurekanat-Biosynthese in Streptomyces viridochromogenes Tü57
… und AviRb), die spezifische Nukleotide der 23S rRNA modifizieren. Verkürzte in-vitro-Transkripte der Domäne V der 23S rRNA dienten als Substrate für Methylierungsversuche mit beiden heterolog exprimierten rRNA-Methyl-transferasen. Durch Primer-Extension und MALDI-TOF-MS-Analytik konnten die …
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Molecular Phylogenetic Analysis of Novel Spiroplasma Isolates
… Australia, and the United States. The 16S rRNA, 23S rRNA, and rpoB genes and the 16S-23S rRNA spacer region were successfully used to establish evolutionary relationships of the closely related spiroplasma isolates. Phylogenetic analyses and non-genetic character mapping indicated that all study …
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Mobile genetic elements in Coxiella burnetii: Friends, foes or just indifferent?
… an intervening sequence (IVS) that interrupt the 23S rRNA gene; an intein within dnaB (encoding replicative DNA helicase) and a homing endonuclease. The introns are self-splicing ribozymes and able to inhibit ribosome function and retard bacterial growth through internal guide sequence …
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Mobile genetic elements in Coxiella burnetii: Friends, foes or just indifferent?
… an intervening sequence (IVS) that interrupt the 23S rRNA gene; an intein within dnaB (encoding replicative DNA helicase) and a homing endonuclease. The introns are self-splicing ribozymes and able to inhibit ribosome function and retard bacterial growth through internal guide sequence …
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Group VIII <em>Spiroplasma</em> of Costa Rica
… Group VIII strains by sequencing the 16S-23S rRNA intergenic spacer region.</p> <p>Spiroplasma cultures were obtained from Costa Rican tabanids and serologically screened. This screening procedure placed ten of the 72 cultures (GSU 5367, 5401, 5404, 5408, 5429, 5431, 5436, 5437, BARC 4898, …
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Studies on a 50S Ribosomal Precursor Particle as a Substrate for <em>erm </em>E Methyltransferase Enzyme in <em>Staphylococcus aureus </em>.
… of erythromycin resistance is the methylation of 23S rRNA by <em>erm</em> methyl transferase enzymes. We are interested in investigating the true substrate for methylation because it is known from our work and the work of others that fully assembled 50S subunits are not substrates for methylation. …
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Histopathological, biological and molecular characteristics of the pathogenic Spiroplasma penaei isolated from the hemolymph of infected Pacific white shrimp, Penaeus vannamei
… based on five partial DNA sequences of 16S rDNA, 23S rDNA, 5S rDNA, gyrB, rpoB genes and two complete DNA sequences of 16S-23S and 23S-5S ISR, were constructed using the distance-based Neighboring-Joining method with Kimura-2-parameter substitution model. The NJ trees based on all DNA sequences …
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Phylogenetic Relationships of Thermophilic Bacteria
… bacteria in early evolution. The 16S and 23S ribosomal RNA genes from the new bacterial isolates were cloned and sequenced. Several types of analysis on the sequences were used to place these extreme thermophiles in the phylogenetic trees of the eubacterial or archaebacterial kingdoms.
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Identification and characterization of the ribosomal RNA operons from Rhodobacter sphaeroides
… DNA sequence analysis has identified the 16S, 23S and 5S rRNAs, two tRNAs (ile and ala) in the 16S-23S spacer region, and an f-met tRNA immediately following the 5S rRNa gene of all three operons. The secondary structure of the rRNA and tRNA molecules present in the rRNA operons was deduced and …
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