Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 153 for “"20s"”.
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Metabolism of Alpha-Synuclein by the 20S Proteasome
… ubiquitin-independent degradation by the 20S core particle of the proteasome. The 20S proteasome is known to degrade proteins with regions of intrinsic disorder, which figure prominently in a wide range of neurodegenerative disorders, including alphaSyn in Parkinson's disease. Importantly, …
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Substrate Selection by the 20S Proteasome and Implications in Disease Pathogenesis
… forms of alpha-synuclein produced by the 20S proteasome in vitro, through a ubiquitin-independent mechanism, are similar to those found in the cingulate cortex of patients with Lewy body diseases. The work presented here describes an investigation into the mechanism by which …
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Cooperativity and communication in archaeal Cdc48·20S, an ancient proteolytic machine
… enzyme and binding to its partner peptidase, the 20S proteasome. In this thesis I present experiments which investigate the role of inter-ring communication in ATP hydrolysis, protein unfolding, and allosteric interactions with the 20S and show how these features affect enzyme function. …
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Compromising the 20S Proteasome Activates a Quality Control Pathway to Mitigate Proteotoxic Stress
… response. To my surprise I identified a core 20S subunit of the proteasome, α1 subunit. This finding uncovered its functional interaction with a yet uncharacterized regulator of proteasome stability in Drosophila, Ecm29 (CG8858). Whereas much is known of proteasome stress response at the level …
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Synthesis of 20S-Hydroxyvitamin D3 Analogs and Their 1α-Hydroxyl Derivatives as Potent Anti-inflammatory Agents
… vitamin D3 by cytochrome P450 enzymes, we found 20S-hydroxyvitamin D3 [20S(OH)D3] as a good lead compound. 20S(OH)D3 suppressed disease symptoms at 2 µg/kg in collagen-induced arthritis model, and high doses of 20S(OH)D3 (up to 30 µg/kg) do not cause hypercalcemia in rats or mice. Thus 20S(OH)D3 …
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Discovery of Novel Tubulin Inhibitors and Selective Survivin Inhibitors for Advanced Melanoma and Total Synthesis of Bioactive 20S-hydroxyvitamin D3
… targets for developing anticancer drugs. 20S-hydroxyvitamin D3 (20S-OH-D3) will be introduced as a promising anti-inflammatory scaffold.</p> <p>Chapter 2 will disclose the SAR study of ABI-231, a previously reported potent tubulin inhibitor from our lab. In this chapter, a new synthetic …
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The proteasome and its ancillary proteins
… recombinantly co-expressed the human P97 and the 20S proteolytic core of the proteasome. Although P97 was shown to co-purify with affinity tagged 20S proteasomes, the data obtained suggest that any direct interaction appears to be of transient nature or may depend on other still unidentified …
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Social variation and change in honorific usage among Korean adults in an urban setting
… sample, ages by design ranged primarily from the 20s through the 50s (with a concentration of speakers in their 20s and 30s). The socioeconomic class distribution by working class (23%) versus middle class (77%) was skewed toward the middle class.
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An Examination of the Motives to Participate in Sprint Distance Triathlon
… age differences in the motives of Affiliation (20s higher than 30s) and Competition (20s higher than 30s). Motivational differences also existed in triathletes based on levels of activity (competitiveness) and levels of experience. In addition, an interaction between age and gender was found and …
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The Role of the Ubiquitin-Proteasome System in the Regulation of Nuclear Hormone Receptor-Dependent Transcription
… transientem siRNA-vermittelten Knockdown von 20S Proteasomuntereinheiten inhibiert. Eine transkriptomweite Genexpressionsanalyse verdeutlichte, dass die Expression vom Großteil der Östrogen-aktivierten Gene durch Proteasominhibition negativ beeinflusst wird. Anschließende Zeitreihen-Analysen …
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Inhibition des 26s Proteasoms : molekularer Mechanismus der Hyperthermie in Prostatakarzinomzellen?
… Proteinextrakt wurde die chymotryptische 20s und 26s Proteasomenaktivität mit Hilfe eines fluorogenen Substrates bestimmt. Nach einer Stunde wurde die DNA-Bindungsaktivität des Transkriptionsfaktors NFkB in EMSAs analysiert. IkBa- und Androgen-Rezeptor-Level wurden durch Western Blot …
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“All the Evil Music Has:” Musical Representations of the Vamp in Interwar American Popular Media
… and American popular music of the 1910s and ‘20s. Abbreviated from vampire, “vamp” came to be used as a term for sexually voracious and predatory women in the early 20<sup>th</sup> century. Following the unprecedented success of the 1915 film <em>A Fool There Was</em>, vamps then became a …
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Development of Surface Plasmon Resonance and Top-Down Mass Spectrometry Approaches to Monitor the Conformational Properties of Amyloidogenic Peptides and Their Enzymatic Cleavage: a Specific Application to Uncover the Mechanisms of Digestion of Branched Ubiquitin-Tau Proteoforms by the Human 20S Proteasome [Sviluppo di approcci di risonanza plasmonica di superficie e spettrometria di massa top-down per monitorare le proprietà conformazionali dei peptidi amiloidogenici e la loro digestione enzimatica: un'applicazione specifica per scoprire i meccanismi di digestione delle proteoforme ramificate di ubiquitina-tau da parte del proteasoma umano 20S]
… 4RD-tau sono state somministrate al Proteasoma 20S umano (h20S). Sono stati quindi applicati esperimenti di SDS-PAGE e spettrometria di massa top-down (MS) per studiare il modello di degradazione delle proteoforme tau e per monitorare il rilascio dei frammenti di digestione. Lo sviluppo di un …
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Structural Studies of the Atypical Rio2 Kinase and N-Acetylglutamate Synthase
… specifically involved in site D cleavage of the 20S rRNA to produce the mature 18S rRNA. Loss of Rio2p is lethal and a decrease of Rio2p activity results in 20S accumulation in the cytoplasm. One of the goals of this thesis was to crystallize Rio2 from a eukaryotic organism in order to determine …
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Effects of 28 days of protein and amino acid supplementation and ankle immobilization on gastrocnemius muscle mass and strength and atrophy- and apoptosis-related gene expression in males.
… was analyzed for mRNA expression of ubiquitin, 20S-HC2, 20S-HC3, E2, E3, atrogin-1, MuRF1, calpain 1, calpain 2, myostatin, TNF-α, and NF-κB. Results: Lower-leg girth decreased over time (p = 0.020), and increased the expression of several genetic variables, but there were no significant changes …
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The use of <i>fuck</i>: A sociolinguistic approach to the usage of <i>fuck</i> in the BNC and blog authorship corpus
… use is most prevalent during the teens and early 20s. This study offers further support of the idea that there are two distinct meanings of <em>fuck</em> that can be separated into two distinct words, <em>fuck1</em> and <em>fuck2</em>, by showing that a non-referential definition of <em>fuck</em> …
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Metal complexes of non-heme ligands: biological applications
… (carbonic anhydrase–I, trypsin, chymotrypsin and 20S proteasome) by non–heme iron complexes, and glutathionylation of non–heme cobalt complexes mimicking the N5 coordination environment like that of biologically important cofactor cobalamin or B<sub>12</sub> (Cbl) are reported. Different non–heme …
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