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Showing 1 to 20 of 22 for “"20-Hydroxyecdysone"”.
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Accumulation pattern of the phytoecdysteroid 20-hydroxyecdysone in different organs of Pffafia glomerata
… por pulsos específicos do zooecdisteróide 20-hidroxiecdisona (20E). Em plantas, a biossíntese, a regulação e a função precisa do fitoecdisteróide 20E permanecem desconhecidas e três hipóteses sobre suas funções potenciais têm sido propostas: i) compostos fitohormonais; ii) compostos …
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Salt stress and UV-B radiation modulate growth, physiology and 20-hydroxyecdysone contents in Brazilian-ginseng [Pfaffia glomerata (Spreng.) Pedersen]
The phytoecdysteroid 20-hydroxyecdysone (20E) is a secondary metabolite with high agrochemical, biotechnological and pharmacological potential, produced only by certain plant species. However, in relation to 20E, it is emphasized that: (i) the biosynthetic pathway is not fully elucidated in plants; …
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The regulation of motoneuron survival in the tobacco hornworm, Manduca sexta
… hemolymph concentrations of a steroid hormone, 20-hydroxyecdysone, and in the case of the MN-12 motoneurons, by actions exerted by neighboring ganglia.
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Measuring juvenile hormone and ecdysteroid titers in insect haemolymph simultaneously by LC-MS: The basis for determining the effectiveness of plant-derived alkaloids as insect growth regulators
… chromatography (HPLC) in less than 20 min using a reversed-phase C18 column and a methanol-water gradient. Subjecting the JH-JHBP (juvenile hormone binding protein) complex to HPLC was sufficient in releasing JH from the JHBP. In order to prevent JH from binding to glass surfaces, it …
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Vitellogenesis in <i>Hyalomma dromedarii</i> (Acari: Ixodidae): A Model for Analysis of Endocrine Regulation in Ixodid Ticks
… Although Juvenile hormone bis-epoxide and 20 hydroxyecdysone treatment did not affect vitellogenin synthesis in cultured fed virgin fat body, they did demonstrate stimulatory activity <em>in vivo</em>. Multihormonal regulation of vitellogenesis, involving a synganglion factor, a juvenoid …
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Molecular Study of Diapause via Generation of Two CDNA Libraries and Partial Cloning of the Ecdysone Receptor in <em>Sarcophaga crassipalpis</em>.
… which is under regulation of the steroid hormone 20-hydroxyecdysone (20-HE). Because the metamorphic system is suspended for diapause in this species, whether or not the EcR is expressed in the diapause state is of interest. To address this issue, a partial EcR was cloned from <em>S. …
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THE EVOLUTION OF PHENOTYPIC PLASTICITY IN BUTTERFLIES
… conclude that sex- specific differences in 20-hydroxyecdysone (20E) hormone titers lead to sexually dimorphic and seasonally plastic sexual ornaments. A comparative study across 13 butterfly species showed eyespot size plasticity in response to rearing temperature is an ancestral trait in …
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Interactions Among Steroid and Xenobiotic Response Cascades in the Regulation of CYP6B1
… with the insect steroid molting hormone 20-hydroxyecdysone in combination with xanthotoxin interfered with induction by xanthotoxin. However, CYP6B1 appears to be regulated at many levels. Treatment with the antioxidant caffeic acid in combination with xanthotoxin enhanced in vitro …
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Identification of juvenile hormone response genes in newly emerged female Aedes aegypti
… pathways often being intertwined with those of 20-hydroxyecdysone (20E). Upon adult emergence, female Aedes aegypti enter a period of development during which they gain competence for mating, bloodfeeding, and egg production. JH levels rise dramatically and peak during the first 2-3 days …
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CHARACTERIZING ECDYSTEROID TITER PROFILES AND THE FUNCTIONAL ROLE OF ECDYSTEROIDS IN ADULT WORKER HONEY BEES (APIS MELLIFERA)
… in the first days of foraging revealed that 20-hydroxyecdysone (20E) treated bees displayed higher levels of foraging activity than vehicle-treated controls, suggesting a novel role for the ecdysteroids present in adult worker bees. I further demonstrated preliminary evidence of a modulatory …
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Phytoecdysteroid accumulation in plants and bioactivities in animal models
… to the evaluation of a continuous infusion of 20-hydroxyecdysone, the predominant phytoecdysteroid, on body tissue composition and skeletal muscle gene expression (Chapters 4 and 5) in mice. The mass of the triceps brachii muscles was significantly increased, however, no differences between …
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Mapping And Characterization Of 18-5 And 12-5, Genes Which Potentially Link The Rhoa Signaling Pathway To The Ecdysone Response
… is regulated both by the steroid hormone 20-hydroxyecdysone (ecdysone) and the RhoA GTPase signaling pathway. Recent evidence suggests that these pathways act cooperatively to control imaginal disc morphogenesis. Thus, leg imaginal disc morphogenesis is an excellent system in which to …
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The Opsins and Nuclear Receptors of the Honey Bee
… to younger workers. Nuclear receptors transduce 20-hydroxyecdysone and juvenile hormone signals to regulate insect development through a coordinated transcriptional cascade. My experiments revealed that the transcriptional cascade characteristic of metamorphosis is active in adult honey bee …
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Induction and Inhibition of a Neuronal Phenotype in Spodoptera Frugiperda (Sf21) Insect Cells
… cells. In the presence of the molting hormone 20-hydroxyecdysone (20-HE), or insulin, in the growth medium, Sf21 cells began to express neuronal morphology, or the production of elongated, axon-like processes within 2-3 days. Maximal differentiation occurred when in the presence of 42 μM 20-HE …
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Endocrine control of fat body composition and effects of the insect growth regulators methoprene and pyriproxyfen on the development and reproduction of the Argentinian cockroach, Blaptica dubia Serville (Blattaria: Blaberidae)
… (JH) III and free ecdysteroids (ecdysone, 20-hydroxyecdysone) were measured in the haemolymph of adult females by the use of HPLC-mass spectrometry. The JH III titer shows significant peaks during vitellogenesis and ovarian growth, and towards the end of the period of gestation. Changes in …
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Breaking the MF curse: the regulatory role of the Methyl farnesoate – MEKRE93 pathway in crustacean molting
… interaction between the steroid hormone 20-hydroxyecdysone (20-E) and the sesquiterpenoid juvenile hormone (JH) control insect molting and development, respectively. On the other hand, crustacean molting is primarily regulated through the endocrine crosstalk between 20-E and the …
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Cytotoxic compounds from the genus Centaurea.
… methanol extract of C. americana also afforded 20-hydroxyecdysone, 24-hydroxyecdysone, lappaol A, arctigenin and a novel compound, 3"-O-caffeoyl(9"'->3")-arctiin. The methanol extract of C. cyanus produced lariciresinol 4-0-B-D-glucoside, berchemol, moschamine and cis-moschamine. Arctigenin, …
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Effets sous-létaux d'un mélange de contaminants d'intérêt émergent sur l'espèce modèle Daphnia magna
… à la survie de cette espèce : l’ecdystéroïde 20-hydroxyecdysone (20E). Ce mémoire présente le développement d’une méthode d’exposition chronique à un des mélanges de contaminants, qui couplée à une méthode d’analyse par chromatographie liquide couplée à la spectrométrie de masse en tandem …
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Underlying mechanisms of juvenile hormone (JH) and its analog in regulating mosquito reproduction
… are controlled by the insect steroid hormone 20-hydroxyecdysone (20E). Kr-h1 seemed to have different roles in regulating the 20E-responsive genes before and after the blood-feeding. RNAi mediated Kr-h1 silencing demonstrated the activator role of Kr-h1 on several 20E-regulated genes in the …
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Factors regulating signal composition and task allocation in colonies of the ant Myrmicaria eumenoides
… concentration. 4) The levels of ecdysone and 20-hydroxyecdysone (found to be the main free ecdysteroids in workers), as well as the sum of the total free ecdysteroids in workers, are only high after eclosion, and then fall to a low, steady level within two weeks. Thus no correlation was found …
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