Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 27 for “"2-photon"”.
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Rapid Two-Photon Holographic Scan Microscopy with Individual Quantum Dots and Its Applications in Biological Samples
Two-photon (2P) excited fluorescence microscopy is particularly advantageous for imaging biological specimens since it offers reduced scattering, deep sample penetration, and intrinsic confocality when excited with point excitation. Here we report the first 2-photon microscopy of individual quantum …
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Advancement of fiber image guide based snapshot imaging spectrometer technology for bioimaging and environmental applications
… integration, optical fiber fabrication using 2-photon 3D printing is presented. 2-photon fabrication of fiber bundles was pursued via investigation of individual fibers and fiber components such as fiber couplers, then small arrays in air, and then scale up and the addition of cladding.
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Spatiotemporal Dynamics of CaMKI During Structural Plasticity of Single Dendritic Spines
… of CaMK1. We monitored CaMKI activity using 2-photon fluorescence lifetime imaging, while inducing LTP in single dendritic spines of rat (Rattus Norvegicus, strain Sprague Dawley) hippocampal CA1 pyramidal neurons using 2-photon glutamate uncaging. Using RNA-interference and pharmacological …
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Construction and calibration of a custom time-of-flight mass spectrometer and its use in measuring the reaction kinetics of transition metal ion-organic interactions.
… ionized fragments. To test the instrument, the 2-photon resonant, 3-photon ionization spectrum of gaseous atomic copper was measured. The (n = 9 – 21) Rydberg series was observed in 2-photon excitation. The term energies of this series converged to copper's lowest ionization threshold with an …
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Analysis of activity-dependent morphological plasticity of dendritic spines on hippocampal neurons
… combination with intracellular recordings and 2-photon-laser-microscopy to spatially restrict the area of induced synaptic activity. My experiments indicate that spine morphology was stable over time with only very little changes occurring following the induction of LTD. The number of large, …
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The role of locus coeruleus norepinephrine in reinforcement learning
… these results, using both opto-tagging and 2-photon imaging we found that LC-NE neurons are active during two task epochs, pre-lever press and post-reinforcement. Pre-press, phasic LC-NE neuronal activity scaled with the degree of certainty of the tone identity, while post-reinforcement LC-NE …
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Interneuron networks and cortical dynamics : emulated whisking drives SOM interneurons in the ketamine anesthetized mouse SI neocortex
… interneuron populations combined with 2-photon imaging, Ca²+-sensitive dyes. Anesthesia and electrical control of facial muscles allowed for naturalistic stimulation without the confounds presented by volitional whisking and unknown top-down or behavioral states. Additional chapters in …
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Novel Single Cell Methods to Identify the Genetic Composition at a Single Nuclear Structure
… sites of double stranded breaks induced by 2-photon irradiation. It is demonstrated that these techniques afford the ability to identify loci occupying a shared site at a single nuclear substructure, and are thus uniquely suited for identifying specific sets of loci that converge non-randomly …
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Tissue-engineered liver microreactor as an in vitro surrogate assay for gene delivery
… culture based on the application of 2-photon microscopy and spectroscopy.
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The Effects of PTEN Deletion on Cell Size and Plasticity in the Hippocampus
… neurons during late development. Using 2-photon imaging, Golgi staining, immunohistochemistry, electron microscopy, and electrophysiologic tools, we determined that late Pten loss does not affect hippocampal development, neuronal or synaptic structure or basic synaptic transmission. …
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Molecular Self-Assembly of Carbon Nanosheets via AFM Nanoprinting
… to enable bottom-up nanofabrication, including 2 photon polymerization (2PP), electrohydrodynamic jet printing, dip-pen nanolithography, and solid-state polymerization among others. In this thesis, we propose a new bottom-up nanofabrication approach by combining molecular self-assembly with atomic …
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Nonlinear Representation of Auditory Stimuli Across the Auditory Cortex
… complex auditory sounds, we performed 2-photon calcium imaging of neural activity of the entire auditory cortex in awake mice, while playing sounds composed of single or multiple frequencies. While we observed simple tuning in response to single frequency stimuli, the calcium responses to …
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A brain-machine interface for investigating neural representations of navigation in a virtual environment
… during completion of a T-maze task using 2-photon calcium imaging, and trained decoders to decode task relevant variables from recorded neural activity. We then closed the loop, and allowed mice to navigate through the virtual maze directly using these brain signals, using the output of our …
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Endoplasmic Reticulum Dynamic Structural Changes in Neurons: The Fission-Fusion Phenomena
… microscopy live cell and tissue imaging, 2-photon laser scanning microscopy and transmission electron microscopy (TEM). The fluorescence imaging was performed on murine primary cultures cotransfected to express cytosolic and ER-specific markers; hippocampal slices from transgenic mice …
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Modelling of the Human Corticospinal Motor Axis and Its Injury Using Organoids
… to look at post-injury axonal growth using 2-Photon imaging to screen growth promoting compounds, validating the potential growth promoting effect of PTEN-inhibition.
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Optimising light-sheet microscopy for high resolution live imaging of the early development of chick embryos
… embryo starts to fold considerably.<br/><br/>2-photon Gaussian light-sheet illumination readily improved image sharpness at a slightly increased penetration depth. In the case of 2-photon Gaussian light-sheet illumination the main concern is caused by the high photo-damage that results in the …
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“Priming” as a novel strategy to enhance chemotherapeutic efficacy
… measured using the SeaHorse XFE Analyser and 2-photon-NADH FLIM. Mitochondrial morphology was assessed with confocal microscopy. To determine whether acetate could prime cells for death, changes in proliferation were measured with the MTT cell viability assay and levels of apoptosis induction …
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Next-Generation Oligonucleotide Probes For Transcriptome In Vivo Analysis (tiva)
… extends the probe’s activation response from 1-photon near-UV light to 2-photon near-IR light. Together, these advancements move TIVA towards a broader range of applications – including deeper regions of challenging, nuclease-abundant tissues – with greater confidence that our construct will …
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Neural Sympathy: Towards Interfaces Compatible with Neural Plasticity
… describe the design of an optical BMI based on 2-photon imaging of CA1 hippocampus in mice. Populations in CA1 are highly plastic and are critical for spatial navigation, providing an excellent testbed for interpreting changes in neural activity induced by BMI usage. Closed-loop experiments with …
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Ultra-rapid 2-D and 3-D laser microprinting of proteins
… a novel high-speed photochemistry based on multi-photon photobleaching of fluorescein, and we build the fastest 2-D and 3-D laser microprinter for proteins to date. First, we show that multiphoton photobleaching of a deoxygenated solution of biotin-4-fluorescein onto a PEG monolayer with acrylate …
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