Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 40 for “"2-oxoglutarate"”.
-
Biochemical and Structural Studies of AlkB: A 2-oxoglutarate Iron(II) Dependent Dioxygenase
… damage repair enzymes. AlkB is a direct repair 2-oxoglutarate iron-II dependent dioxygenase that oxidizes alkyl lesions occurring on the N1 position of purines and the N3 position of pyrimidines in both DNA and RNA. AlkB preferentially repairs 1-methyladenine and 3-methylcytosine lesions, but it …
-
Molecular Studies to Investigate 4-hydroxy-2-oxoglutarate Metabolism Defects of Primary Hyperoxaluria Type 3
… PH3 results when mutations prevent 4-hydroxy-2-oxoglutarate aldolase (HOGA) from cleaving 4-hydroxy-2-oxoglutarate (HOG) into pyruvate and glyoxylate. It is proposed that the consequences of PH3 could be lessened by preventing conditions that favor oxalate production downstream of deficient HOGA …
-
Defining the role of 4-hydroxy-2-oxoglutarate aldolase in hydroxyproline metabolism and primary hyperoxaluria
Aberrant glyoxylate metabolism is a hallmark of primary hyperoxaluria (PH). PH types 1 and 2 are the consequence of functional defects in alanine-glyoxylate aminotransferase (AGT) and glyoxylate reductase (GR), respectively. The resulting increase in oxalate excretion can lead to the formation of …
-
Molecular and Biochemical Genetics of 2-Oxoglutarate-Dependent Dioxygenases Required for Flavonoid Biosynthesis in Arabidopsis thaliana
Three 2-oxoglutarate-dependent dioxygenases required for flavonoid biosynthesis were characterized in Arabidopsis thaliana. Genes encoding flavanone 3-hydroxylase (F3H), flavonol synthase (FLS), and leucoanthocyanidin dioxygenase (LDOX) were cloned and sequenced. The predicted proteins encoded by …
-
Examination of 2-Oxoglutarate Dependant Dioxygenases Leading to the Production of Flavonols in <i>Arabidopsis thaliana</i>
… and pharmacological activities in animals. The 2-oxoglutarate-dependant dioxygenases(2-ODDs), flavonol synthase (FLS) and flavanone 3-hydroxylase (F3H), are essential for flavonol synthesis. The primary goal of this study has been to gain a deeper understanding of the biochemistry of these enzymes …
-
GC-MS Investigation of Metabolic Activity in 4-Hydroxy-2-Oxoglutarate Aldolase Deficiency of Primary Hyperoxaluria Type 3
… by a malfunction in the enzyme 4-hydroxy-2-oxoglutarate aldolase (HOGA), which catalyzes the cleavage of 4-hydroxy-2-oxoglutarate (HOG) to form glyoxylate and pyruvate. Since the enzymatic malfunction occurs before glyoxylate formation, and previous studies have shown HOG to be elevated in …
-
Escherichia coli 2-oxoglutarate dehydrogenase multienzyme complex: e1 and e2 substrate specificty, e1 carboligase activity, and e2 interchain succinyl transfer
Escherichia coli (E. coli) 2-oxoglutarate dehydrogenase multienzyme complex (OGDHc) contains three components: a thiamin di phosphate (ThD P) dependent 2-oxogl utarate dehydrogenase (E1 o), a di hydrol i poylsucci nyl transferase (E2o), and a di hydrol i poyl dehydrogenase (E3). The first two …
-
Biochemical and Genetic Characterization of Reduced Phosphorus Metabolism in Pseudomonas Stutzeri WM88
… that htrA encodes a novel member of the 2-oxoglutarate dependent dioxygenase enzyme family, htxBCDE encode a binding protein dependent hypophosphite transporter and that htxF-htxN encode the components of a Carbon-Phosphorus lyase for the degradation of phosphonate compounds. Sequence …
-
The role of ABHD11 in the regulation of the hypoxia inducible transcription factors
… members of a diverse group of enzymes termed 2-oxoglutarate-dependent dioxygenases. These enzymes all require oxygen, iron and the Tricarboxylic Acid (TCA) cycle metabolite, 2-oxoglutarate (2-OG/a-ketoglutarate) for catalytic activity. Therefore, understanding how 2-OG levels are regulated is …
-
Shared Metabolic Pathways in Fuel-Stimulated Insulin Secretion
… in the pyruvate/isocitrate cycle is cytosolic 2-oxoglutarate (2OG). In an effort to better understand the components of the pyruvate/isocitrate cycle and the signals that it generates, we initially focused our studies on the transporter protein responsible for the return of 2OG to the …
-
Structural insights into conformationally-gated reactions catalyzed by thiamine pyrophosphate-dependent enzymes
… with carbonyl carbon(s), such as pyruvate and 2-oxoglutarate, two central metabolites. This thesis presents structural analyses of three TPP-dependent enzymes. Most interestingly, each mechanism of three proteins studied contains a gated step that is drastically accelerated by 3-5 orders of …
-
In situ Metabolism and Biogeography of Phototrophic Consortia
… were strongly attracted by sulfide and 2-oxoglutarate, which indicated a potential role of these compounds in the metabolism of "P. roseum". In chemocline water samples, 2-[14C(U)]-oxoglutarate was utilized at nanomolar concentrations (half saturation constant of uptake Kt ≤ 10 - 40 nM), …
-
Characterising mechanisms of regulation of hypoxia-inducible factors.
… hypoxia, such as iron chelators and analogs of 2-oxoglutarate, prevented hydroxylation of the asparagine allowing the CAD to interact with the transcriptional coactivator protein p300. We then demonstrate that the protein factor inhibiting HIF-1 (FIH-1), previously shown to interact with HIF, is …
-
Lipoic acid assembly on 2-oxoacid dehydrogenases in E.coli
… We report that the intact pyruvate and 2-oxoglutarate dehydrogenase complexes specifically copurify with both LipB and LipA. Proteomic, genetic and dehydrogenase activity data indicate that all of the 2-oxoacid dehydrogenase components are present. In contrast LplA, the lipoate protein …
-
A Mutant of Arabidopsis Thaliana Deficient in Chloroplast Dicarboxylate Transport Activity
… oil filter centrifugation technique. Malate, 2-oxoglutarate, glutamate and aspartate uptake were severely reduced or missing in chloroplasts from the mutant.
-
Mechanisms of Enzyme Neofunctionalization in Plant Specialized Metabolism
… two enzyme families: nonheme iron(II)- and 2-oxoglutarate-dependent halogenase and BAHD acyltransferase. First, we describe the genomic- and biochemical-basis for gene duplication and neofunctionalization of flavonol synthase (FLS) that led to the unique emergence of halogenase in …
-
Characterization of Reduced Phosphorus Metabolism in Alcaligenes Faecalis WM2072 and Xanthobacter Flavus WM2814
… an NAD:phosphite oxidoreductase (PtxD) and 2-oxoglutarate-dependent hypophosphite dioxygenase (HtxA). HtxA and PtxD were shown to be required for the utilization of phosphite and hypophosphite by A. faecalis. This organism also has the htxBCD genes that comprise an ABC type transporter, …
-
Methodological and biological studies of the amino acid oxidases.
… to the NALH dependent reductive amination of 2-oxoglutarate, the reaction being catalysed by exogenous glutamate dehydrogenase L-glutamate: NAL(P) oxidoreductase (deaminating), EC 1.4.1.3.). The inhibition of L-amino acid oxidase (L-amino acid: oxygen oxidoreductase (deaminating), EC 1.4.3.3.) …
Page 1 of 2