Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 9 of 9 for “"2-dimensional gel electrophoresis"”.
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Hibernation is super complex: dynamics of electron transport system supercomplexes
… strengths, and quantified SC abundance using 2-dimensional gel electrophoresis and immunoblotting. Rats had fewer SCs than TLGS. SCs are dynamic in hibernation and the complex III composition of different SCs differed between hibernation states and seasons. There was no correlation between SC …
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Study of the Reproducibility of Proteomics Methods and Variability of Fruit Fly Proteomes.
… of proteomic biomarker research that is largely unexplored is the comparative reproducibility of certain methods such as two-dimensional gel electrophoresis and liquid chromatography/mass spectrometry. In particular, with liquid chromatography/mass spectrometry, it is not known whether …
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Important role of the type VI secretion system in the virulence of an emergent human pathogen Aeromonas hydrophila: Delineating the mechanism of action of its effectors
… cytotoxicity in eukaryotic cells. Based on 2-dimensional gel electrophoresis and mass spectrometric analyses of bacterial supernatants, we identified a member of the VgrG protein family, (VgrG1), containing a vegetative insecticidal protein (VIP-2) domain at its carboxyl-terminal end, as well …
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Proteomic analysis of the olfactory mucosa, thoracic aorta, urinary bladder, and descending colon of smooth muscle alpha actin null mutant mice
… adaptive and maladaptive changes. Through 2-dimensional gel electrophoresis and mass spectrometry, we found increased abundance of four proteins in the olfactory mucosa of SMAA null mice: alpha tubulin, precursors of vomeronasal secretory proteins I and II, and lipocalin 13 precursor. In the …
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Investigating the molecular biology of aging and extended longevity: Proteomic and genomic analysis of mouse liver
… the ER/mitochondrial fraction was resolved by 2-dimensional gel electrophoresis and then assayed for oxidative damage as indicated by protein carbonylation. MALDI/TOF analysis and N-terminal sequencing of these proteins identified BiP/Grp78, protein disulfide isomerase (PDI), and calreticulin as …
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Proteomic analysis of diurnal variation in human skeletal muscle performance
… is required. Proteomic techniques including 2-dimensional gel electrophoresis, high- performance liquid chromatography and peptide mass spectrometry can be used to investigate changes in the abundance of hundreds of proteins simultaneously. To date, such techniques have not been used to …
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Proteomic analysis of cellular models of neurodegeneration and mitochondrial dysfunction
… were then fractionated using an optimised 2-dimensional gel electrophoresis (2DE) protocol and the reproducibility of the method was investigated. A preliminary study comparing the mitochondrial proteome profile from two different states of mouse N2a neuroblastoma cells, mitotic and …
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The physiological response of the white-rot fungus, Schizophyllum commune to Trichoderma Viride, during interspecific mycelial combat
… both species paired against each other using 2- Dimensional gel electrophoresis, and differentially expressed proteins were identified by MALDI-TOF-MS/MS, following peptide fragmentation. Proteins involved in protein synthesis and assembly, unfolded protein response, response to cellular injury, …
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Caratterizzazione proteomica di fluidi e tessuti in diverse condizioni fisio-patologiche
… three groups, according to image analysis of the gels, were submitted to in-gel tryptic digestion and the peptide mixture analyzed by high performance HPLC-ESI-MS/MS for their characterization. By this strategy, we identified three over-expressed proteins in atterm newborns with respect to preterm …