Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 24 for “"2`-deoxyuridine"”.
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Synthesis of Novel 5-Aryl 2’-Deoxyuridine and 2’-Deoxycytidine Analogues
… of the 3’- and 5’-hydroxyl groups of 5-iodo-2’-deoxyuridine. Next, the installation of a phenyl ring at the C5 position of the nucleobase via a Suzuki-Miyaura cross-coupling reaction, yielding 5-phenyl-2’-deoxyuridine (5-PdU). After that, modification of the obtained 5-PdU at the C4 position was …
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From coarse fate choice to precise pattern: post-mitotic progenitor targeting
… patterns. To address this, 5-ethynyl-2’-deoxyuridine (EdU) labeling and plug transplant techniques were used to trace progenitor movement in non-irradiated planarians. Using whole-mount fluorescence in situ hybridization (FISH) and the quantification of EdU-labeled cells, this study …
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The in vivo proliferation kinetics of human solid tumours
… to take sequential biopsies. The drug 5-bromo-2-deoxyuridine (BRdU) is a safe, ethical alternative to tritiated thymidine for in vivo labelling of DNA during the S phase of the cell cycle. The label can be detected by conventional histochemistry, and by multiparameter flow cytometry. The latter …
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뇌종양 동물모델에서의 인간신경줄기세포주의 종양으로의 특이적 이주에 관한 입체적 분석
… of rats 8 8. Labeling of F3 Cells with 5-bromo-2-deoxyuridine 8 9. Hematoxylin and Eosin stain 9 10. Beta-galactosidase immunofluoresecence 9 11. Statistical Analysis 10 Ⅲ. RESULTS 11 1. Tumor-tropism pattern of NSCs 11 2. Migration rate of NSCs 19 3. NSC migration studied by stereology 23 4. …
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The effect of doxorubicin on the U2OS cell cycle
… levels of synthesis after analysis with Bro mo-2-deoxyuridine (BrdU) and Propidium Iodide (PI) staining. The cells are unable to recover from the severity of this damage and become apoptotic. When a 2 [mu]M dose is applied to the cells, a G2 arrest occurs. This is shown by lower levels of Cyclin B …
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Development and characterization of an inducible RNAi system in Toxoplasma gondii
… uracil incorporation assay and 5-fluro-2'-deoxyuridine resistance. Subsequently, the inducible RNAi vector was used to elucidate the role of microneme protein 3 (MIC 3), which is an invasion related gene. We generated a stable transgenic parasite line in which the expression of MIC 3 was …
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Liver fluke development: a target for parasite control?
… localised using thymidine analogue 5-Ethynyl-2´-deoxyuridine (EdU). This thesis further characterises these neoblast-like stem cells, investigating their dynamics, supportive roles in growth and development and identifying putative regulatory effectors. This study reports increased juvenile …
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Nucleoside and oligonucleotide approaches towards potential HIV chemotherapies
… of nucleoside analogues derived from uridine, 2'-deoxyuridine and AZT. Crystal structures of six compounds were successfully determined. Anti-HIV activity was observed for most compounds in the series although none were without cytotoxicity. The most active compound of the series was the ribose …
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T cell mediated satellite cell function: implications for age-associated changes in skeletal muscle regeneration
… anti-CD28. Co-stimulation increased 5-bromo-2'-deoxyuridine (BrdU) incorporation (proliferation) of T cells from 13.382% (SEM=4.55, n=5) in control to 64.77% (SEM= 6.02, n=5). Additionally, T cell cytokines increased MPC proliferation by 23.98% (SEM=5.69, n=4) in young MPCs but decreased by …
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Developmental Appearance of Phosphorylated Intermediate Filaments in Nuclei of Glioma and Neuroblastoma Cells
… DNA synthesis using uptake of 5'-bromo 2'-deoxyuridine. Using immunocytochemistry, Jl-31 and SMI-31 mAB nuclear labeling was exhibited primarily in proliferating cells with diminished nuclear labeling in quiescent cells found at high densities. SDS-PAGE and western blot analysis on whole …
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Proliferation, Migration, and Survival of Cells in the Telencephalon of the Ball Python, Python regius
… of this trait. The thymidine analog 5-bromo-2’-deoxyuridine (BrdU) was used to quantify cell proliferation, migration, and survival in the ball python (<em>Python regius</em>). First, to determine the proper dose of BrdU for injection we subcutaneously injected 50mg/kg, 100mg/kg, and 250mg/kg …
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Cell Growth Paramenters and Sister Chromatid Exchange Level in Fibroblasts from a Patient Diagnosed with Bloom Syndrome
… with the thymidine analog 5-bromo-2'- deoxyuridine. Following culture, cells were harvested and stained for differentiation of the sister chromatids using a fluorescence plus Giemsa technique, A mitotic index for each cell line was determined by counting the number of cells in division …
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Behavioral and genetic characteristics of intestinal cell lineages in health and disease
… rates at a single-cell level using 5-ethynol-2'-deoxyuridine (EdU) pulse-chase labeling. While we observed marked differences in the proliferation rates between the absorptive and secretory lineages (the former is faster), our experimental setup was unable to resolve any possible differences in …
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Effects of Homocysteine on Cardiac Neural Crest Cell Formation
… effect of Hcys on cell proliferation, 5-bromo-2'-deoxyuridine (BrdU) was used to label cells in S- phase of the cell cycle. In control embryos, a significantly higher percentage of newly formed NC cells were in S-phase compared to migrating NC cells or their progenitors within the neural tube. …
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Equine Trophectoderm Cells and Their Role in Fetal-Maternal Recognition
… HGF, FGF-2 or IGF-1 for 24 hr, with 5-ethynyl-2'-deoxyuridine (EdU) supplementation during the final 2 hr. Subsequently, cells were fixed and EdU positive and total nuclei were enumerated. A parallel plate of iTr cells was treated in a similar manner and lysed for total RNA isolation. Quantitative …
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Investigation of the mechanisms underlying the effects of hyperglycaemia on cardiac structural and electrical remodelling
… dUTP nick-end labelling (TUNEL) and 5-ethynyl-2-deoxyuridine (EdU) assay. Results: Undifferentiated mESCs were positive for pluripotent transcription factors Nanog and Oct3/4, whereas the cardiac differentiated mESCs were positive for cardiac proteins troponin T, α-actinin 2, connexin 43, …
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The Roles of the Collagen Binding Integrins in the Regulation of Endothelial Cell Function
… by cell number quantification and 5-ethynyl-2’-deoxyuridine (EdU) incorporation analysis, was unaffected by inhibition or siRNA knockdown of any integrin receptor. Knockdown or inhibition of α2β1 severely hindered HUVEC attachment and spreading to collagen and THPs, measured by static and …
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Role of protein kinase C isozymes in cellular senescence and reversal of senescence in response to 12-O-tetradecanoylphorbol-13 acetate (TPA) treatment in
… young cells. Furthermore, increased 5-bromo-2'-deoxyuridine (BrdU) incorporation at the mitochondrial nucleoid along with mitochondrial transcription factors A (TFAM). Nevertheless, we observed that mitochondria dysfunction was increased via alteration of ROS level, integrity of membrane …
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Investigating the effects of enhanced catecholamine stimulation in stem cell-derived cardiomyocytes
… blot, immunocytochemistry, and the 5ethynyl-2'-deoxyuridine (EdU) stain cell-proliferation assay. Results: Isoprenaline stimulation induced a four-fold increase in the EB beating rate compared to the untreated or EBs treated with the β-adrenergic receptor antagonist, pindolol (0.2μM), indicating …
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