Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 11 of 11 for “"2'-O-methylation"”.
-
Subversion of innate antiviral immunity by SARS-CoV-2: An example from the study of nonstructural protein 16.
… IFN-stimulated genes that sense a lack of 2’-O methylation, partially restores fitness to the NSP16 mutant. Conversely, overexpressing IFIT1 either alone or in combination with IFIT3 attenuates the NSP16 mutant relative to wild-type. Finally, we demonstrate that sinefungin, a MTase inhibitor …
-
STRUCTURAL AND FUNCTIONAL STUDIES OF ARCHAEAL BOX C/D GUIDE RNA AND ROLE OF A PUTATIVE HUMAN PSEUDOURIDINE SYNTHASE, PUS10 IN APOPTOSIS
… stability and functional diversity. RNA methylation is a very common type of post-transcriptional modification and is present in all domains of life: Archaea, Eukaryotes and Bacteria. Some of these methylations are catalyzed either by a RNA-protein complex or by stand-alone enzymes. The …
-
A TALE OF TWO METHYLATION MODIFICATIONS IN ARCHAEAL RNAs
… sugars of the individual ribonucleotides. 2'-O-methylation of ribose sugars and isomerization of Uridines to Pseudouridines are two most predominant modifications in rRNAs and tRNAs across all domains of life. Besides 2'-O-methylation of ribose sugars, methylation of pseudouridine (Ø) at …
-
GUIDE RNA-DEPENDENT AND INDEPENDENT tRNA MODIFICATIONS IN ARCHAEA
… L7Ae (a core protein associated with archaeal methylation guide RNPs) binding motif in sR-tMet. ψ54, the near universal modification found in TψC stem-loop of archaeal tRNAs is catalyzed by the protein Pus10. An earlier study from our laboratory had shown that Pus10 from two different …
-
Discovery and characterization of novel RNA repair systems
… from T4 systems by performing 3’-terminal 2’-O-methylation during RNA repair, which prevents the repaired RNA from repeated cleavage at the same site. Bacterial Pnkp and Hen1 appear pair-wise in the same operon in approximately 5% of known bacterial species. Although the bacterial Pnkp has been …
-
Nucleolar Stress Due to Depletion of Nopp140 in Drosophila melanogaster
… in variable amounts; RT-PCRs showed that 2’-O-methylation of rRNA in Nopp140-/- cells was reduced. Ultra structural analysis showed that Nopp140-/- cells were deficient in cytoplasmic ribosomes, but instead contained abnormal electron dense cytoplasmic granules. Furthermore, metabolic labeling …
-
The analysis of organic matter in oil-shales
… pyrite content and not kerogen reduction. 2. O-methylation in the presence of a phase transfer catalyst. - By the removal of hydrogen bond interactions via O-methylation, it was possible to determine the contribution of such secondary interactions to the insolubility of the kerogens. Problems …
-
An Inverse Electron-Demand Diels-Alder Strategy for Mapping RNA Methylation
… such as N6-methyladenosine (m6A), 2'-O-methylation (Nm), and 5-methylcytosine (m5C), are critical regulators of gene expression. Mapping these modifications at single-nucleotide resolution, however, remains a major challenge as current methods often suffer from low resolution or …
-
Investigations into the role of RNA modification in the function of the c-Myc IRES
… cytoplasm and recent data have shown that m6A methylation alone, which occurs in the nucleus, is sufficient to promote internal ribosome entry. Therefore, a testable hypothesised is that the “nuclear” event required for IRES function is RNA modification. Prior data from a yeast 3 Hybrid screen …
-
Minor form of human 5.8s ribosomal RNA
… also heterogeneity with respect to the degree of methylation in rodent 5.8s rRNA. The conformation of the two chain length isomers is influenced by 2'-O-methylation of the uridylic acid residue at position 14, i.e. the most compact conformation is not ribose methylated in that position. The …
-
Strukturní a funkční charakterizace inhibice flavivirové methyltransferázy.
Viry rodu flavivirus, patřící do rodiny Flaviviridae, jsou rozšířenou skupinou virových patogenů, které představují globální hrozbu pro lidské zdraví. Následky infekce jsou neurologické nebo jiné onemocnění jako je encefalitida, meningitida, mikrocefalie nebo krvácivá horečka, přičemž přímá …