Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 57 for “"18S rRNA"”.
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Characterization of Yeast 18S rRNA Dimethyl Transferase, Dim1p
… process involves numerous cleavages of pre-rRNA, modification of nucleotides, and concomitant assembly of the ribosomal proteins onto the rRNA. Considerable information is available about the importance of conserved pre-rRNA cleavage events in ribosome biogenesis; however, very little is …
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Use of an 18s rRNA metagenomics approach as a method of detection of multiple infections in field blood samples collected on FTA cards from cattle
… by amplifying the variable the V9 region of the 18s sequence using primers designed to target multiple eukaryotic sequences. A number of protocols were modified and optimised in order to elute a sufficient quality and quantity of DNA to use for NGS. Due to the age of the FTA cards, the samples …
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Using PCR Amplification and Genetic Sequence Analysis of 18S rRNA Genes to Survey the Microbial Diversity and Distribution of Eukaryotic Microbes Inhabiting Two Thermo-acidic Streams in Yellowstone National Park, Wyoming
… approach, sequence analysis of 18S rRNA genes PCR-amplified from environmental DNA, was used to explore the diversity and distribution of eukaryotic microbes inhabiting algal mats in two acidic geothermal streams in Yellowstone National Park. The objectives were to: (1) clarify …
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Evaluation of translation initiation features of satellite tobacco necrosis virus RNA
… of some heterologous transcript mRNAs. An 18S ribosomal RNA complementary region in the 5$\sp\prime$-untranslated leader of STNV RNA may be involved in an 18S rRNA/STNV RNA hybridization mechanism of translation initiation. Translational studies of $\beta$-glucuronidase transcripts with …
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Molecular detection of the apicomplexan parasite 'X' (APX) in bivalves in New Zealand
… and combating the disease. Two unique APX 18S rRNA gene sequences (~1.8kb) were isolated from APX in flat oysters by firstly applying generic apicomplexan primers followed by sequencing, and then further extension of the obtained sequences using specific primers. BLASTN search of the …
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The Role of Ribosomal Maturation Proteins WBSCR22 and EMG1 Methyltransferase Activity in Cancer
… 1639 and pseudouridine 1248 residue of the 18S rRNA and they are known as WBSCR22 and EMG1. Both proteins are key factors for ribosome biogenesis and previous studies have demonstrated that in this context, they both act as scaffolds and that their catalytic activity is dispensable for 18S …
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The Mechanism of Cap-Independent Initiation of Translation of Satellite Tobacco Necrosis Virus RNA
… to the 3$\sp\prime$-terminal region of the 18S rRNA.
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The use of genetic materials, RNA, to determine the age of saliva stains
… of the housekeeping genes (β-actin mRNA and 18S rRNA) that were used in a previous study assessed on bloodstains. In the second pilot approach, the relationship assessment was carried out between the saliva's age and the relative quantities of two different-sized segments on the same …
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Molecular Detection for the Apicomplexan Parasites Cyclospora cayetanensis and Cryptosporidium spp.
… PCR protocol comprised the amplification of the 18S rRNA gene. To optimize the Cryptosporidium spp. PCR assay, a first step was to identify positive samples among donated specimens from a Honduran epidemiological study on STH. Initial screening was done with an enzyme immunoassay (copro-antigen …
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Detection of Babesia species in domestic and wild Southern African felids by means of DNA probes
… amplification of the variable region (V4) in the 18S rRNA gene using genus-specific primers where one is labelled with biotin. This is followed by a blotting step, where the amplicons are hybridized to oligonucleotides bound to a nitrocellulose membrane. The third and last step is the detection of …
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A MARINE PATHOGENIC PROTIST ACROSS SCALES: HOST-MICROBIOME INTERACTION, PROVENANCE, MORPHOLOGY AND TAXONOMICAL CHARACTERIZATION OF THE SCUTICOCILIATE PARASITE OF DIADEMATIDAE
… bacterial and archaeal microbiome profiling, 18S rRNA phylogenetics, and scuticociliate ultrastructural characterization, this collection of work aims to elucidate to intricacies of Diadema antillarum scuticociliatosis (DaSc) and the Philaster-clade (DaScPc) ciliate etiological agent. A …
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Molecular analysis of algal communities in the San Joaquin River
… (PCR) primers were designed to amplify the 18S rRNA gene of certain taxonomic groups of freshwater algae; there was limited success in specific amplification. Additionally, a primer pair utilizing both the 16S plastid gene and the 16S rRNA gene was tested with success, amplifying both …
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Evolutionary patterns within the Anthozoa (phylum Cindaria) reflected in ribosomal gene sequences
… of octocorals and amplifying the nuclear 18S ribosomal RNA gene. The DNA sequence for the complete 18S rRNA gene can then be determined. Techniques were designed to solve several problems for obtaining genetic sequences from museum specimens. The DNA extractions of museum specimens yielded …
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Bovine embryo microinjection, culture, microsurgery, and DNA analysis by the polymerase chain reaction technique
… efficiency of PCR amplification of an endogenous 18S rRNA. Mean development scores (l1=degenerate, 2=morula, 3=blastocyst) and mean cell number after microsurgery and 48 h of culture did not differ between treatments (P>.05; 2.4 ± .1 and 41.8 ± 2.5 versus 2.3 ± .1 and 48.8 ± 2.9, respectively). …
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Isolation And Characterization Of Soil Microorganisms Capable Of Utilizing Used Motor Oil For Growth
… microorganisms were identified by either the 16S rRNA gene, or the 18S rRNA gene DNA sequences. Classical methods of identification were performed as a supplementation to the molecular method. The isolates were analyzed for their ability to degrade various hydrocarbons, such as n-alkanes, branched …
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The Isolation and Characterization of the Microbial Flora in the Alimentary Canal of <em>Gromphadorhina portentosa</em> Based on rDNA Sequences.
… for Eubacterial, Archaeal, and Eukaryotic 16s/18s rRNA were then used for PCR amplification of total DNA isolated from gut contents. Sequences from isolates were compared using BLAST, ClustalW, and other programs to recognize the isolates' identities and place them using a phylogenetic tree …
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Entwicklung von neuen Nachweismethoden für Legionellen und Amöben und ihre Anwendung in ökologischen Studien
… für die in situ Hybridisierung eine neue 16S rRNA-gerichtete Sonde LEGPNE1 entwickelt, die spezifisch ist für L. pneumophila. In Versuchsreihen mit verschiedenen Bakterienkulturen wurden die Spezifität und Sensitivität der Gensonde ermittelt. LEGPNE1 erwies sich als artspezifisch und erkannte …
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SCREENING FOR POTENTIAL ANTIBACTERIAL AGENTS AND THE APPLICATION OF ISOTOPIC LABELS FOR THE CHARACTERIZATION OF THEIR EFFECTS ON THE DYNAMICS OF THE BACTERIAL 16S RIBOSOMAL RNA
… construct of the bacterial 16S ribosomal RNA (rRNA). We show that, at high concentration, these compounds interfere with bacterial growth. We also observed that the use of efflux pump inhibitors in combination with the fragments enhanced their antibacterial properties and may help combat …
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Resolving phylogenetic relationships within the order Enoplida (Phylum Nematoda)
… identifications, nuclear gene sequences (18S and 28S rRNA), and mitochondrial gene sequences (Cox1) were obtained from marine specimens representing a variety of deep-sea and intertidal habitats. Molecular data were used to assess the phylogenetic placement of the Enoplid clade, resolve …
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Characterization of features of the initiation of translation of satellite tobacco necrosis virus RNA
… made without a cap. STNV RNA may have a unique 18S ribosomal RNA hybridization site that allows internal initiation of translation to occur. This site was tested by inserting a translation initiation codon upstream of the internal initiation site. Interestingly, the initiation of translation of …
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