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Showing 1 to 20 of 145 for “"18S"”.
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Characterization of Yeast 18S rRNA Dimethyl Transferase, Dim1p
Eukaryotic ribosome biogenesis, a dynamic and coordinated multistep process which requires more than 150 trans-acting factors, has been intensely studied in the yeast Saccharomyces cerevisiae. This evolutionarily conserved process involves numerous cleavages of pre-rRNA, modification of …
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Tetrapod phylogeny as inferred from 18S and 28S ribosomal-RNA sequence comparisons
The superclass Tetrapoda consists of several well defined monophyletic taxa: Anura, Urodela, Gymnophiona, Testudinata, Squamata, Rhychocephalia, Crocodilia, Aves and Mammalia. The relationships among these groups, however, is controversial. Neither paleontological evidence nor morphological, …
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A molecular phylogeny of the families and orders of Collembola (Arthropoda: Hexapoda) using the 18S rDNA gene
In this study I investigate the phylogenetic relationships of the families and orders of Collembola, with special reference to six groups that traditionally have been problematic, or that have become problematic with the implementation of the cladistic philosophy to the systematics of the group. …
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Use of an 18s rRNA metagenomics approach as a method of detection of multiple infections in field blood samples collected on FTA cards from cattle
… by amplifying the variable the V9 region of the 18s sequence using primers designed to target multiple eukaryotic sequences. A number of protocols were modified and optimised in order to elute a sufficient quality and quantity of DNA to use for NGS. Due to the age of the FTA cards, the samples …
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Using PCR Amplification and Genetic Sequence Analysis of 18S rRNA Genes to Survey the Microbial Diversity and Distribution of Eukaryotic Microbes Inhabiting Two Thermo-acidic Streams in Yellowstone National Park, Wyoming
… approach, sequence analysis of 18S rRNA genes PCR-amplified from environmental DNA, was used to explore the diversity and distribution of eukaryotic microbes inhabiting algal mats in two acidic geothermal streams in Yellowstone National Park. The objectives were to: (1) clarify …
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Evaluation of translation initiation features of satellite tobacco necrosis virus RNA
… of some heterologous transcript mRNAs. An 18S ribosomal RNA complementary region in the 5$\sp\prime$-untranslated leader of STNV RNA may be involved in an 18S rRNA/STNV RNA hybridization mechanism of translation initiation. Translational studies of $\beta$-glucuronidase transcripts with …
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Integrated Study Of Dinoflagellate Diversity In The Gulf of Naples
… (HTS) metabarcoding of the V4 region in the 18S rDNA. To taxonomically identify the metabarcode sequences, I established a database, called DinoREF, of taxonomically verified and nomenclaturally updated 18S reference barcodes with associated metadata. The reference sequences were organised …
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Citogenética clássica e molecular de formigas neotropicais
… FISH para mapeamento físico de genes ribossomais 18S em 10 espécies de formigas neotropicais com representantes em três subfamílias (Ponerinae, Formicinae, Myrmicinae), além do emprego da técnica de coloração com o fluorocromo CMA 3 , com o intuito de verificar a composição de bases desses genes. …
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Evolução cariogenômica em peixes recifais das famílias Acanthuridae (Acanthuriformes) e Holocentridae (Holocentriformes)
… staining and repetitive sequence mapping of 18S rDNA, 5S rDNA, histones H3 and H2B-H2A, microsatellites (GA)15, Tol2 transposons and Rex3 retrotransposon through fluorescent in situ hybridization. Between Acanthuridae, A. triostegus showed a karyotypic pattern considered basal for Percomorpha …
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Comparative cytogenetics in the genus Trifolium section Trifolium (clover) : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Plant Biology at Massey University
… FISH revealed a unique distribution pattern for 18s and 5s rDNA with the 5s and 18s signals present on the satellited chromosome pair only. For 5s rDNA, hybridisation sites were observed in three areas of the satellited chromosome, two of those sites were on either side of the 18s signal. …
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Caracterização cariotípica de cinco espécies de Meliponini da Região Amazônica
… com o número de marcações observadas para o rDNA 18S obtidos pela tecnica de FISH, exceto para a espécie N. punctata, em que foram obtidas duas marcaçoes CMA3+ e duas marcações rDNA 18S. Em S. bipunctata e Partamona sp. foram encontradas seis marcaçoes CMA3+ e duas marcações rDNA 18S. Através da …
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A case study-based investigation into the factors that influence the physical and technical interactions on match performance within an under 18's academy football team.
… soccer. Using 19 males from a single under 18s Premier League soccer club, 7 matches (5 home, 2 away) were used for analysis. Physical performance indicators used in this study include: individual total distance; high speed running; sprint efforts; accelerations; and decelerations. Technical …
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Resolving phylogenetic relationships within the order Enoplida (Phylum Nematoda)
… identifications, nuclear gene sequences (18S and 28S rRNA), and mitochondrial gene sequences (Cox1) were obtained from marine specimens representing a variety of deep-sea and intertidal habitats. Molecular data were used to assess the phylogenetic placement of the Enoplid clade, resolve …
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Phylogeny of parasitic wasps of Torymidae (Hymenoptera: Chalcidoidea) and evolution of their life-strategies
… of sequencies of two ribosomal genes (18S rDNA and 28S rDNA) were developed for 649 species of chalcidoid taxa. However, family Torymidae was considered as polyphyletic group with the subfamily Megastigminae unrelated to the subfamily Toryminae (paper I). Monophyly of Torymidae was …
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Characterization of features of the initiation of translation of satellite tobacco necrosis virus RNA
… made without a cap. STNV RNA may have a unique 18S ribosomal RNA hybridization site that allows internal initiation of translation to occur. This site was tested by inserting a translation initiation codon upstream of the internal initiation site. Interestingly, the initiation of translation of …
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Molecular detection of the apicomplexan parasite 'X' (APX) in bivalves in New Zealand
… and combating the disease. Two unique APX 18S rRNA gene sequences (~1.8kb) were isolated from APX in flat oysters by firstly applying generic apicomplexan primers followed by sequencing, and then further extension of the obtained sequences using specific primers. BLASTN search of the …
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Evolutionary patterns within the Anthozoa (phylum Cindaria) reflected in ribosomal gene sequences
… of octocorals and amplifying the nuclear 18S ribosomal RNA gene. The DNA sequence for the complete 18S rRNA gene can then be determined. Techniques were designed to solve several problems for obtaining genetic sequences from museum specimens. The DNA extractions of museum specimens yielded …
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The Role of Ribosomal Maturation Proteins WBSCR22 and EMG1 Methyltransferase Activity in Cancer
… 1639 and pseudouridine 1248 residue of the 18S rRNA and they are known as WBSCR22 and EMG1. Both proteins are key factors for ribosome biogenesis and previous studies have demonstrated that in this context, they both act as scaffolds and that their catalytic activity is dispensable for 18S …
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The Production and Characterization of Xenopus Albumin Complementary-Dna Clones and Regulation of Rna Levels by Estrogen and Dexamethasone
… albumin cDNA clones were isolated from an 18S cDNA library produced from uninduced RNA. Several albumin clones were identified and characterized with respect to their restriction maps, and homology to each other. Clones corresponding to both 74,000 and 72,000 dalton albumin were isolated. …
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