Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"17D"”.
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Characterization of a recombinant chimeric yellow fever 17D vaccine - dengue-4 virus
… based on a backbone of the yellow fever virus 17D (YFV-17D) vaccine virus with the structural components premembrane and envelope (prME) of a corresponding wild-type DENV. This thesis investigated the impact of viral chimerization on a YFV-17D infectious clone where the 17D prME genes were …
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Contributions of Structural and Non-structural Genes to the Attenuation of the 17D Vaccine
Live-attenuated 17D vaccine, developed to control Yellow Fever virus (YFV), was derived by serial passage of wild-type Asibi strain 176 times in mouse and chicken tissues. Despite the derivation of 17D over 75 years ago, the mechanism of attenuation is still poorly understood. The genomes of …
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Investigating the role of non-structural genes in the genotype and phenotype of yellow fever vaccine strain 17D
… embryo tissue. The resulting vaccine strain, 17D, gave rise to all substrains in use today. Strain 17D and the resulting substrains, 17DD and 17D-204, were found to differ from Asibi virus by 20 amino acid substitutions with nine substitutions in the structural proteins of the virus and the …
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Investigation of the interplay of yellow fever virus structural protein epitopes and genetic diversity
… by a live attenuated vaccine (LAV), strain 17D, that was derived from serial passage of the wild type (WT) strain Asibi in chicken tissue. The mechanism of attenuation of 17D is incompletely understood. This dissertation investigates the contribution of the envelope (E) protein in YFV …
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Using machine learning to increase the predictive value of humanized mouse models for the human immune response to YFV-17D
… to the live attenuated yellow fever vaccine (YFV17D). Additionally, semi-supervised machine learning algorithms have been shown to further uncover translational insights from mouse models. I hypothesized that these strategies may be synergistic when combined, further improving the predictive …
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Evaluation of arthropod-borne virus recombination potential and phenotype: In vitro and in vivo analysis of flavivirus and alphavirus model systems
… (SINV)) and flavivirus (yellow fever virus (YFV) 17D) recombinant crosses. Standard molecular cloning methodologies were used to generate CHIKV, SINV, and YFV 17D replicon/defective helper systems possessing trans-complementary sequences necessary and sufficient for the generation of full length …
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Characterization of the molecular and biological determinants of viral dissemination from the mosquito midgut: Yellow feber virus in Aedes aegypti
… strain, and the attenuated, non-disseminating 17D vaccine strain. These viruses were characterized in vitro in Vero green monkey kidney cells and C6/36 Ae. albopictus mosquito cells and in vivo for virus production, infection and dissemination in orally infected Ae. aegypti. Data from these …
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Population Structure of Yellow Fever Virus: Influence of Viral Diversity on Vaccine Attenuation
… Asibi, and a commercial aliquot of the YFV 17D-204 vaccine. In silico methods revealed the vaccine to be of lower diversity than the parental strain, leading to subsequent hypotheses that the level of complexity in the vaccine populations would be related to phenotypic stabilities. A deep …
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Immunogenicity and toxicity of yellow fever vaccines : a systematic review
… and is derived from an attenuated virus strain 17D originally produced in the 1930s. The vaccine has historically been considered effective and safe. However, severe life-threatening side effects to the vaccine have been reported in the past 20 years. Acute vaccinerelated viscerotropic (AVD) and …
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Characterization of a novel bioluminescent reporter protein for the non-invasive preclinical imaging of viral infection
… century, a live attenuated strain of the YFV (17D) was deemed as an effective vaccine against the mosquito-borne virus. Despite this progress, the virus persists in endemic regions of Africa and South America, necessitating ongoing study and surveillance. Continued research into the virus's …
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Dipyrazolylphosphanes in Condensation and P–N/P–P Bond Metathesis Reactions
… compounds as stated by the few examples reported.17d,20 The application of pyrazolyl-substituted phosphanes in P–P bond formation reactions is a relatively young field of research.21 Their synthesis and general chemical behavior as well as advantages in comparison to dialkylamino-substituted …
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Seed protein, oil, and yield of soybean genotypes with high and normal oleic acid concentration
… mutant genes, mutant S117N FAD2-1A allele from 17D and mutant P137R FAD2-1B allele from PI283327. Thirty five F4:7 high oleic lines with these mutant FAD2-1A and FAD2-1B genes were compared to thirty five F4:7 normal or wild type oleic acid lines for seed contents of the five fatty acids common …
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Computational metric embeddings
… metric space into Rd within a factor of Q(n1/(17d)). Finally, we consider the problem of embedding into tree metrics. We give a 0(1)approximation algorithm for the case where the input is the shortest-path metric of an unweighted graph.