Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"17Beta-Estradiol"”.
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Modulation of humoral immunity in the rat by 17beta-estradiol and prolactin
… Many studies have shown that prolactin (PRL) and estradiol (E$\sb2$) augment immunity. Given that E$\sb2$ is a potent physiologic stimulus for the release of PRL from the pituitary, it is plausible that PRL mediates the influence of E$\sb2$ upon the immune system. To test this hypothesis, we …
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Development and Comparison of 17beta-Estradiol Sorption Isotherms for Three Agriculturally Productive Soils From Different Physiographic Regions in Virginia
Natural steroid estrogens such as 17beta-estradiol in low nanogram per liter concentrations can adversely affect the reproductive health of aquatic organisms. The overall goal of this research was to quantify the sorption of 17beta-estradiol to three soils considered to be agriculturally productive …
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The Addition of Melatonin to 17beta-Estradiol and Progesterone Modulated Markers Relevant to Mammary Tumor Development and Modulated Steroid Receptors Relevant to Uterine Protection in a Mouse Model of Breast Cancer
… that a novel replacement therapy containing 17β-estradiol (E2) and half the recommended dose of progesterone (P4) along with nocturnal melatonin supplementation (EPMRT) would be protective against mammary tumor development. Previously, in a mouse model of HER2+ breast cancer, our laboratory …
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The Identification and Characterization of Estrogen Receptors in the Mouse and Human Lens and Their Role in Cataract Development
… receptors are expressed in the lens, [125I]-17beta-estradiol binding and mRNA expression of ERalpha, ERbeta, and GPR30 were examined in the mouse and human lens; 2) to determine if the loss of ERalpha and/or ERbeta receptors will induce spontaneous development of cataracts and to examine …
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Studies of Atherosclerosis in Systemic Lupus Erythematosus
… we treated Sle/LDLr-/- mice with 5.6 µg/day of 17beta -estradiol or placebo for 10 weeks. We found that that estrogen treatment resulted in decreased atherosclerotic plaque size without improvement of plasma lipoprotein concentrations and acceleration of the SLE phenotype. For aim three, we used …
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Estrogen Inducible Proteinase Inhibitor 9 Protects Target Cells From Immune Surveillance and Apoptosis
… cells. In MCF-7 cells, induction of PI-9 by 17beta-estradiol (E2) inhibited TRAIL-mediated cytotoxicity. This is the first demonstration that regulation of PI-9 expression from its endogenous promoter results in production of sufficient PI-9 to inhibit cytotoxocity by members of the TNF …
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DNA Repair, Redox Regulation and Modulation of Estrogen Receptor Alpha Mediated Transcription
… of estrogen receptor alpha (ERalpha) with 17beta-estradiol (E2) facilitates binding of the receptor to estrogen response elements (EREs) in target genes, which in turn leads to recruitment of coregulatory proteins. To better understand how estrogen-responsive genes are regulated, our …
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Estrogen Receptor -Dependent and Estrogen Receptor-Independent Pathways for Tamoxifen and 4-Hydroxytamoxifen-Induced Programmed Cell Death
… of OHT activate an ER-dependent pathway. 17beta-estradiol, raloxifene, and ICI 182,780 are all effective competitors, which block OHT-ER and Tam-ER-dependent cell death. Since the p38-specific inhibitor SB203580 blocks OHT-ER induced cell death, stress kinase pathways are likely involved. …
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A Novel Link Between Oxidative Stress Proteins and Estrogen Receptor Alpha-Mediated Gene Expression
The classical genomic pathway for 17beta-estradiol (E 2) action involves binding to estrogen receptor alpha (ERalpha) in target cells, interaction with estrogen response element (ERE)-containing DNA, recruitment of coregulatory proteins, and modification of estrogen responsive genes. Because …
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Age, growth, and reproductive biology, of the winter skate, Leucoraja ocellata, in the western Gulf of Maine
… of the sex steroids testosterone (T), 17beta-estradiol (E2), and progesterone (P4) were determined by radio immunoassay and compared to morphological and histological changes occurring in the reproductive tract over a complete reproductive cycle for mature individuals. Overall, when the …
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Engineering Estrogen Receptor-Based Gene Switches and a Superoxide Dismutase for Therapeutic Applications
… molecule ligand for the wild type hERalpha - 17beta-estradiol. Furthermore, the two created receptor-ligand pairs do not show significant cross-interaction with each other. Both created receptor-ligand pairs have the potential to be used as gene switches for the selective regulation of gene …
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Protein Engineering via in Vitro Coevolution
… structural bridge between the natural ligand 17beta-estradiol and the target ligand corticosterone, were chosen to assist the directed evolution of hERalphaLBD. A total of approximately 106 randomly point-mutated variants were phenotypically screened in four rounds of directed evolution, …
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Development and Application of a Fluorescence Anisotropy Microplate Assay for Analyzing the Interaction of Steroid Hormone Receptors With DNA and Coactivators
… complex occurred only in the presence of 17beta-estradiol (E2), as previously reported. Differential inhibition of ERE-ER interaction with SRC1a was observed with different NR-Box peptide competitors. Furthermore, the profile of peptide-mediated inhibition differed between ERE-ERalpha and …
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Estrogen action in the myocardium
… In ovarectomised animals treated either with 17beta-estradiol alone or in combination with a specific estrogen receptor antagonist, ICI 182780, it was shown that both alpha-MHC mRNA and protein were upregulated by estrogen in an estrogen receptor specific manner. The in vivo results were …
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The effect of hypoxia on ER-β expression in the lung and cultured pulmonary artery endothelial cells
17-β estradiol (E2) exerts protective effects in hypoxia-induced pulmonary hypertension (HPH) via endothelial cell estrogen receptor (ER)-dependent mechanisms. However, the effects of hypoxia on ER expression in the pulmonary-right ventricle (RV) axis remain unknown. Based on previous data …
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Cytochrome P450 1B1 and 12/15 Lipoxygenase Interaction in Paraventricular Nucleus in Angiotensin II-induced Hypertension in Females
… P450 (CYP)1B1-generated metabolite of 17β-estradiol (E2) (2-methoxyestradiol, 2-ME) and ALOX15 in the paraventricular nucleus (PVN) as a mechanism that protects against Ang II-induced hypertension and its associated pathogenesis in female mice. <strong>Methods:</strong> Experiments were …
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Effects of Long-Term Exposure of Normal C57BL/6J Inbred Mice to 17β-Estradiol on Gene Expression in Lymphocytes: mRNA Analysis of Lymphokines and bcl-2/fas
… the effect of long-term exposure to 17β-estradiol on lymphokine and bcl-2/fas (proto-oncogenes) mRNA expression. We evaluated the effects of estrogen on the expression of genes for lymphokines, which are essential for the immune response. It is hypothesized that estrogen may regulate the …