Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 44 for “"16s rRNA gene sequencing"”.
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BIOINFORMATIC PIPELINES AND MICROBIAL DATABASES FOR THE ANALYSIS OF DAIRY COWS 16S RRNA-GENE SEQUENCING DATA: A BENCHMARKING STUDY.
… risultati utilizzando tre diversi database - 16S-ITGDB, RPD, SILVA e Greengenes -, di cui due in due differenti aggiornamenti (SILVA versione 132 e versione 138, Greengenes versione 13.9 e versione 2). Le prestazioni di queste combinazioni saranno testate utilizzando due comunità fittizie e …
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Comparison of Subterranean Termite (Rhinotermitidae: Reticulitermes) Gut Bacterial Diversity Within and Between Colonies and to Other Termite Species Using Molecular Techniques (ARDRA and 16S rRNA Gene Sequencing)
… amplified rDNA restriction analysis (ARDRA) and 16S rRNA gene sequencing. It was determined that ARDRA was an effective technique for characterizing the diversity of the termite gut microbiota. Of the 512 clones analyzed in the ARDRA study, 261 different ARDRA profiles were found. Forty-two 16S …
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Prevalence of the related treponematoses syphilis and yaws and co-occurrence of pathogens causing similar skin lesions in Ghana
… diseases, two Treponema pallidum subspecies, are genetically similar, but they have always been studied independently. In this context, this study aimed to assess the prevalence of these treponematoses and determine the co-occurrence of pathogens causing similar skin lesions in the Ashanti region …
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American Alligator Fecal Coliform and Pathogen Identification, Genotypic Fingerprinting, and REP-PCR Bacterial Source Tracking in South Carolina
… the API 20E biochemical test system and 16S rRNA gene sequencing methods, in the identification of alligator cloacal isolates. The second component evaluated the ability of Repetitive Extragenic Palindromic PCR (REP-PCR) to produce identifiable, unique, and stable genomic fingerprints …
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Microbiology of the sulfur-dominated glacial ecosystem at Borup Fiord Pass, and high Arctic low-temperature spring associated microbial mats, The
… analyzed for microbial community composition (16S rRNA gene sequencing), function (metagenomic sequencing), and aqueous geochemistry. 16S rRNA gene sequencing data revealed Flavobacterium was common to almost all samples. Sulfur-oxidizing microorganisms (Sulfurimonas, Sulfurovum, and …
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Enumeration, isolation, identification and probiotic characterisation of lactic acid bacteria from Nigerian human breast milk
… PCR (rep-PCR). A total of nineteen groups were generated from the rep-PCR DNA profiles. This was followed by genotypic identification using 16S rRNA gene sequencing. The 16S rRNA gene sequencing identified LAB and non-LAB. Most of the LAB isolates belonged to the Lactobacillus genus, the rest …
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The effect of tylosin exposure or exclusion on liver abscess prevalence, fecal pathogen populations, and the microflora of finished beef products from feedlot cattle
… carcasses, and finished beef products through 16S rRNA gene sequencing and shotgun sequencing from feedlot cattle with and without exposure to tylosin. Overall, liver abscess rates were lower among cattle exposed to tylosin. However, there were no differences among treatment groups for any of …
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ANALYSIS OF MICROBIOME IN VARIOUS HUMAN MEDICAL CONDITIONS USING NEXT-GENERATION SEQUENCING OF THE 16S rRNA GENE
… in 43 healthy and diseased patients using 16S rRNA gene sequencing. We identified a rich microbial landscape, identifying over 338 bacterial taxa. Notably, <em>Acinetobacter</em>, <em>Cutibacterium</em>, <em>Acidovorax</em> and <em>Herbaspirillum</em> were prevalent in the ocular samples …
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A study of clinical strains of Pseudomonas Aeruginosa and the investigation of antibiotic resistance mechanisms in the multidrug resistant strain PA13
… Biolog GN. Their identification was confirmed by 16S rRNA gene sequencing. The antibiotic resistance profiles of the isolates were determined against forty-one antibiotics belonging to eleven distinct classes. All the isolates were resistant to penicillin G, ampicillin, cephalothin, cloxacillin, …
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Microfluidic cell culture chambers with nanoporous walls for chemical communication
Reconstruction of phylogenetic trees based on 16S rRNA gene sequencing reveals that so far only a tiny fraction of microbial diversity has been cultured in the laboratory. One major reason behind this "unculturability" is that many microbes function in symbiosis, frequently exchanging metabolites …
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Screening and evaluation of bacteria for the biological control of Botrytis cinerea on Vitis vinifera
… The bacterial isolates were identified using 16S rRNA gene sequencing. Various experiments assessed the modes of action behind the inhibitory activity, including testing for cell wall degrading enzymes (glucanases, proteases, chitinases), evaluating competition for space and nutrients via …
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Drivers And Dynamics Of Harmful Algal Blooms: Understanding Nitrogen Fixation And Bloom Composition In Minnesota Temperate Lakes
… blooms and in lake water columns in general. Sampling was conducted across six Minnesota lakes spanning a range of physical, chemical, and biological characteristics, capturing conditions during bloom events and in non-bloom periods. Nitrogen fixation rates were quantified using the …
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The mucosal microbiome of inflammatory bowel diseases
… new methodology for the taxonomic assignment of 16S rRNA gene sequencing data, then utilises this and other cutting edge techniques to elucidate the mucosal associated microbiome in Inflammatory Bowel Disease and finally examines the viability of using mucosal samples for shotgun metagenomics or …
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Investigation of Microbiota in Health and Disease of Poultry
… health and diseases of both humans and animals. 16S rRNA genes sequence analysis is one of the most popular and commonly used methods in the analysis of microbiotas associated with hosts. In this dissertation, the microbiotas of chickens (broilers, breeders, and layers) and turkeys were evaluated …
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The house dust microbiota in the Drakenstein Child Health Study
… the bacterial composition of house dust using 16S rRNA gene sequencing and 4) determine those factors influencing the bacterial composition of house dust by performing bioinformatic and data analysis on the sequenced dust samples. Methods: In order to study the microbial content of house dust, …
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High-throughput sequencing to understand pathogen carriage and transmission
Genomic sequencing is a powerful technology in the study of pathogen biology and pathogen transmission. In this thesis I have studied the human nasal carriage of *Staphylococcus aureus* and, in response to the SARS-CoV-2 pandemic, I examine the transmission of SARS-CoV-2, two critically important …
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Using Culturomics to Characterize the Gastrointestinal Microbiota of Donors for Patients with Bipolar Disorder Experiencing Depression
… identified using MALDI-TOF mass spectrometry, 16S rRNA gene sequencing, and Sanger sequencing. Bacterial community profiles from fecal samples were characterized using shotgun metagenomics. From the donor stool, 78 different species of bacteria were isolated across 43 genera including …
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DEHYDRATION AND MICROBIAL IMPACTS ON WATER UPTAKE AND POSTHARVEST QUALITY OF CUT LILY
… stem, especially in the lowest 15 cm. By using 16S rRNA gene sequencing, we found different flowers (rose, lily, and gerbera) affect the composition of microbial community, and the diversity of microbial communities increased over time in the vase period. In addition, bacterial taxa and …
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Microbial instrumentation utilizing microfluidics
Reconstruction of phylogenetic trees based on 16S rRNA gene sequencing reveals abundant microbial diversity in nature. However, studies of microbiology have been limited by the capabilities to replicate the natural environment or artificially manipulate cells. Advances in microbial instrumentation …
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Biogeochemical Cycling of Manganese in Drinking Water Systems
… Mn-oxidizing and -reducing culture media. 16S rRNA gene sequencing revealed that most are bacteria of the Bacillus spp. (i.e., Bacillus pumilus and Bacillus cereus). These bacteria are capable of performing Mn-oxidation and -reduction under controlled laboratory conditions. Pseudo-first …
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