Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 49 for “"16S rRNA sequencing"”.
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Identification, Characterization, and Use of Precipitation-borne and Plant-associated Bacteria
… source of the phyllosphere microbiome. Using 16S rRNA amplicon and metagenomic sequencing, I identified the genera Massilia, Sphingomonas, Methylobacterium, Pseudomonas, Acidiphilium, and Pantoea as members of the core rain microbiome in Blacksburg, VA. Further, I used rainwater as a bacterial …
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Changes in the microbial community of Lubomirskia baicalensis affected by Red Sponge Disease
… of sick and healthy sponges through the use of 16S rRNA sequencing. Sick sponge microbiomes shared a conserved group of taxa while the healthy sponge microbiomes had greater diversity. Indicator species analysis identified two significant taxa in the sick sponges that are acidophilic. There was …
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Microbial communities as predictors of outcomes in industrial and clinical applications
… surveys of diversity. In this thesis, I use 16S rRNA sequencing analysis to measure community composition across time series to predict outcomes for three applications: bioreactor function; a non-invasive diagnostic of endometriosis; and commercial chicken rearing. I identify bacteria that …
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Proteomics as a tool for the characterisation of nosocomial pathogens
… contamination errors that corroborated with 16S rRNA sequencing. By contrast, C. difficile isolates were only partially identified (to the genus level) using the MMU database and protocol. Changes in the matrix solution and use of the new database (SARAMIS™) resulted in the correct …
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Necrobiome Succession in Muscle Tissue as a Potential Indicator of Postmortem Interval
… during human decomposition. Utilization of 16s rRNA sequencing provided information used to assess the overall diversity and variability of the necrobiome community composition over the course of the decomposition process for three donors. Human remains were donated to Forensic Research …
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Effects of 2015 earthquake on biological stability of water in Nepal
… damage) using microbial source tracking and 16S rRNA sequencing. Samples from the same locations were taken before the earthquake in summer 2014, and then again in May to July, 2015 and in Dec. 2015, corresponding to one month and eight months after the 2015 earthquake. Microbial source …
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Enhancing Economic and Environmental Value in Soybean Production through Value-Added Innovation
… patterns. Microbial community analysis through 16S rRNA sequencing identified specific taxa associated with each gas. Overall, soil carbon, nitrogen, and base cations showed positive relationships with GHG-positive microbes, while iron and several micronutrients correlated with GHG-negative …
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Development of a sensor-based rapid microbial testing platform for the Irish meat industry
… together with the analysis of these samples by 16S rRNA sequencing. Respirometric profiles revealed unusual linear profiles for pork and lamb mince, the origins of which remain to be investigated. In addition to respirometric microbiological assessment, the whole microbiome of each mincemeat …
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Soil Microbial Community Response to Human Decomposition in Michigan's Upper Peninsula
… DNA extraction, samples were sequenced using 16S rRNA sequencing, processed with QIIME 2, and microbial community changes analyzed with R using alpha and beta diversity metrics and relative abundance.</p> <p>Results revealed that human decomposition significantly alters the soil microbiome …
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Endophytic Actinomycetes as potential agents to control common scab of potatoes
… level identification of the endophytes, the 16S rRNA gene was sequenced. To assess inhibition abilities of endophytes against the pathogen, double layer antibiotic assays were done by spot inoculating endophytes and overlaying agar with the pathogen. Multiple layer agar plates were used to …
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Effects of 5��-Dihydrotestosterone (DHT) on Mouse Gut Microbiome– A Study of Sex Differences and Hormonal Effects on Gut Microbiome Composition
… DNA from intestinal samples were subjected to 16S rRNA sequencing. Sequence reads were analyzed using MicrobiomeAnalyst and Piphillin<em>, </em>two web-based programs for phylogenetic and functional analysis. We identified a significant increase in <em>Bacteroides acidifaciens </em>in …
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16S rRNA sequence analysis of silver carp microbial community in eDNA water samples
… For this, DNA was extracted and analyzed through 16S rRNA sequencing on the Illumina MiSeq platform yielding 25 million reads and over 16,000 OTUs after initial clean up. Following analysis, there were multiple microbial groups, in particular 127 OTUs within the Fusobacteria phylum previously …
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Coupling Metaproteomics with Taxonomy to Determine Responses of Bacterioplankton to Organic Perturbations in the Western Arctic Ocean
… functioning and taxonomic composition over time. 16S rRNA sequencing was employed to confirm bacterial taxonomic dynamics. These approaches were linked to particulate analyses for lipids and amino acids in order to track temporal changes in organic substrate composition. Results obtained using …
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Group VIII <em>Spiroplasma</em> of Costa Rica
… analysis used for spiroplasmas has been 16S rRNA sequencing. The primary goal ofmy research was to evaluate serologically Group VIII spiroplasmas isolated from tropical Costa Rican tabanids (Diptera: Tabanidae) and to compare them to the temperate North American Group VIII spiroplasmas. A …
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Cycling of Iron and Manganese (Oxyhydr)oxides in the Presence of Organic Matter
… differ considerably from controls based on 16S-rRNA sequencing data. These ferrihydrite-amended communities continue to diverge from other experiments over time despite a convergence in methane production. In Chapter 2, we characterize Mn cycling in Siders Pond (Cape Cod, MA). We find Mn …
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Identifying hosts of bacteriophage transduction in microbial communities with RNA-addressable modification
… RAM will splice an RNA barcode onto the host’s 16S rRNA. Sequencing of barcoded 16S rRNA can then reveal host information for a given MGE. RAM has previously been demonstrated in plasmids delivered to a community via conjugation. For the first time we demonstrate deployment of RAM in phages and …
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Molecular Profiling of Antibiotic Resistant Bacteria and Genes in Raw and Drinking Water
… of the isolates was determined by bacterial 16S rRNA sequencing and results revealed that isolates belonged to the families Enterobacteriaceae, Paenibacillaceae, Bacillaceae, Yersiniaceae, Xanthomonadaceae and Flavobacteriaceae that of which some members are of clinical importance. Data …
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The Effects Of The Estrus Cycle On Microbiota Composition And The Opportunistic Pathogen Listeria Monocytogenes Infection Process In Murine Models
… gut microbial diversity was determined using 16S rRNA sequencing on 22 cecum samples from female CD1 mice. We determined that Firmicute bacteria are prominent at all stages of the estrus cycle; however, increased Firmicute density was observed at the estrus and diestrus stages (DE), dominating …
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The microbiology of chronic suppurative otitis media
… in a prospective study using swabs. Bacterial 16S rRNA sequencing identified the polymicrobial nature of CSOM and the presence of a microbiome in healthy ears, previously thought to be sterile. The results from molecular methods did not support culture-based findings. The inflammatory response …
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Role of the Gut Microbiome in Cancer Immunotherapy Efficacy and Side Effects
… evaluate anti-CTLA-4-induced gut inflammation. 16S rRNA sequencing identified specific bacterial taxa associated with gut toxicity (Dubosiella and genera from Lachnospiraceae) or protection (UBA7173 and Paramuribaculum). Genera from Lachnospiraceae and Oscillospiraceae were linked to reduced ICI …
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