Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 233 for “"16S rRNA gene"”.
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ANALYSIS OF MICROBIOME IN VARIOUS HUMAN MEDICAL CONDITIONS USING NEXT-GENERATION SEQUENCING OF THE 16S rRNA GENE
… in 43 healthy and diseased patients using 16S rRNA gene sequencing. We identified a rich microbial landscape, identifying over 338 bacterial taxa. Notably, <em>Acinetobacter</em>, <em>Cutibacterium</em>, <em>Acidovorax</em> and <em>Herbaspirillum</em> were prevalent in the ocular samples …
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BIOINFORMATIC PIPELINES AND MICROBIAL DATABASES FOR THE ANALYSIS OF DAIRY COWS 16S RRNA-GENE SEQUENCING DATA: A BENCHMARKING STUDY.
… risultati utilizzando tre diversi database - 16S-ITGDB, RPD, SILVA e Greengenes -, di cui due in due differenti aggiornamenti (SILVA versione 132 e versione 138, Greengenes versione 13.9 e versione 2). Le prestazioni di queste combinazioni saranno testate utilizzando due comunità fittizie e …
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Development of a 16S rRNA gene sequence as a biomarker specific to the gastrointestinal tract and feces of swine based on suppressive subtractive hybridization
… DNA extraction was performed followed by 16S rRNA amplification. The amplified DNA was taken into suppressive subtractive hybridization (SSH) to enrich for swine specific sequences. The final products obtained in SSH were cloned and sequenced. Swine-specific primers were designed and …
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T-RFLP analysis of bacterial 16S rRNA gene sequences isolated from river otter (Lontra canadensis) scat and parasite screening for the presence of Toxoplasma gondii
… with the MOBIO PowerSoil™ DNA Isolation Kit and 16S rRNA gene sequences were amplified from each sample. After digestion with Mspl, TRFLPs were analyzed in an ABI Prism™ 310 Genetic Analyzetin triplicate and data peak information from each electropherogram was uploaded into the Phylogenetic …
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Comparison of Subterranean Termite (Rhinotermitidae: Reticulitermes) Gut Bacterial Diversity Within and Between Colonies and to Other Termite Species Using Molecular Techniques (ARDRA and 16S rRNA Gene Sequencing)
… amplified rDNA restriction analysis (ARDRA) and 16S rRNA gene sequencing. It was determined that ARDRA was an effective technique for characterizing the diversity of the termite gut microbiota. Of the 512 clones analyzed in the ARDRA study, 261 different ARDRA profiles were found. Forty-two 16S …
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Redutive Dehalogenation of Chlorinated Alkanes by Novel Bacteria at the PetroProcessor of Louisiana Inc. Superfund Site
… were not dehalogenated. Phylogenetic analysis based on 16S rRNA gene sequences showed these isolates to represent a new lineage within the Chloroflexi. Their closest previously cultured relatives are “Dehalococcoides” strains with 16S rRNA gene sequence similarities of only …
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Laccases from actinomycetes for lignocellulose degradation
… between 30 and 37oC. Analysis of the 16S rRNA gene sequences of the strains showed that strain MS26 had the highest sequence similarity (99%) to Streptomyces atrovirens strain NRRL B-16357 and Streptomyces viridodiastaticus strain IFO 13106. Strain DFNR17 had the highest 16S rRNA gene …
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Detection of an Introduced Bacterial Culture in Greywater Treatment Reactors
… using molecular tools. Analysis of the 16S rRNA gene by polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE) revealed community profiles of the dominant bacterial species. Species and strainspecific primers targeting either the 16S rRNA gene or the intergemc …
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Prevalence of the related treponematoses syphilis and yaws and co-occurrence of pathogens causing similar skin lesions in Ghana
… diseases, two Treponema pallidum subspecies, are genetically similar, but they have always been studied independently. In this context, this study aimed to assess the prevalence of these treponematoses and determine the co-occurrence of pathogens causing similar skin lesions in the Ashanti region …
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Isolation and Ecology of Bacterial Populations Involved in Reductive Dechlorination of Chlorinated Solvents
… of enrichment culture dechlorination kinetics, 16S rRNA gene based analyses, and reductive dehalogenase gene targeted PCR approaches revealed that complete reductive dechlorination is carried out by multiple dechlorinators. Two new dechlorinating species were isolated from contaminated and …
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Diet Induced Alteration of the Murine Intestinal Microbiome After Antibiotic Ablation
… by Gram stain, quantitative flow cytometry, next generation sequencing of the V3 or V4 regions of the 16S rRNA gene, fluorescence microscopy, and sequence analysis of the tuf gene, which can distinguish among Streptococcus and Staphylococcus genera.;Results: Fecal samples from antibiotic-treated …
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Profiling Bacterial Community Dynamics For Wildlife Ecology and Geological Mineral Exploration
… of microbiome environments. Bacterial community 16S rRNA gene amplicon sequencing is an approach to microbiome exploration that profiles community composition, structure, and dynamics across environmental conditions. In this thesis, 16S rRNA gene amplicon sequencing is used to investigate the …
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USE OF MOLECULAR APPROACHES TO STUDY THE OCCURRENCE OF EXTREMOPHILES AND EXTREMODURES IN NON-EXTREME ENVIRONMENTS
… low pH values were isolated and identified using 16S rRNA gene cloning, PCR amplification and phylogenetic analysis. Detailed analysis of their halophilic and alkaliphilic physiology for adaptation in the environment was investigated using nuclear magnetic resonance spectroscopy (NMR) to detect …
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Analysis of actinobacterial biodiversity in reservoir sediment and cave soil and screening of isolates for antimycobacterial activity
… successfully identified to the genus level by 16S-rRNA gene analysis: one Micrococcus strain, one Streptacidiphilus strain, one Micromonospora strain and 26 Streptomyces strains. The phylogenetic position of each identified strain within its genus was investigated by generating a phylogenetic …
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A comparative study of the origins of cyanobacteria at Musina Water Treatment Plant using DNA fingerprints
… and some of its tributaries based on the phylogenetic analyses of 16S rRNA gene. The Musina water treatment plant is located downstream of a weir and the Beit bridge on the Limpopo River and the raw water supply is abstracted from 22 boreholes of which 14 are along the Limpopo River and 8 …
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Molecular characterization of Flavobacterium spp. isolated from Rainbow trout (Oncorhynchus mykiss) farmed in Southern Africa and development of a PCR-based tool for differentiating between isolates
… study was to elucidate the genotypic and phylogenetic diversity of the Flavobacterium spp. associated with these fish and to develop a new molecular system for rapid and accurate identification and differentiation of the isolates. In order to do this, the genotypic and phylogenetic diversity of …
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Distribution of Dehalococcoidia in marine sediments and strategies for their enrichment
… Earth. Microbial community studies based on the 16S rRNA gene have revealed that bacteria of the class Dehalococcoidia, phylum Chloroflexi, are one of the most widespread and abundant bacteria inhabiting the marine subsurface. However, their physiology and ecological role are unknown. In this …
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Metagenomic guided isolation of sporoactinomycetes from soil and screening for antimycobacterial activity
… University of Cape Town (UCT). Fifty (50) and 52 16S-rRNA gene sequences were amplified for the K and TC samples, respectively, from environmental DNA (eDNA) using actinobacterium-specific primers. EzBioCloud analyses of the 16S-rRNA gene sequences showed a total of 10 orders present in each of …
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Gut-associated microbial symbionts of the marsh fiddler crab, Uca pugnax
… this thesis was to characterize the ecology and genetic diversity of resident gut microbes to advance our understanding of their interactions with their host, the marsh fiddler crab, Uca pugnax. Furthermore, we assessed whether microbes benefit the host by contributing extracellular enzymes along …
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Analysis of Actinobacterial Biodiversity in Marine Sediment from Gericke's Point (South Africa) and Screening of Isolates for Novel Antimycobacterial Compounds
… strains and one Nocardia strain. The partial 16S-rRNA gene sequences obtained for each confirmed actinobacterial isolate were used to determine their phylogenetic positions within their respective genera. Further investigation of specific isolates was done utilising the gyrB gene to determine …
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