Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"16S rRNA amplicon sequencing"”.
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Microbial transformation of arsenic in Bengal floodplain
… paddy soil in Bangladesh. In the present study, 16S rRNA amplicon sequencing and metagenomics (Illumina), alongside chemical analysis in paddy and nonpaddy soils, from Holocene and Pleistocene regions, of Bangladesh to investigate microbial composition and functional diversity of arsenic …
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THE BROWN BEAR GUT MICROBIOME: A NOVEL TOOL FOR CONSERVATION & MANAGEMENT
… and Preserves and extracted microbial DNA for 16S rRNA amplicon sequencing and microbial taxonomic classification. My results indicate that location and diet drive GMB variation and GMB composition reflects diverse foraging strategies while allowing brown bears to achieve similar body condition …
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Effect of Soil Type, Composting, and Antibiotic Use on Fate of Antibiotic Resistance Genes and Microbial Community Composition in Dairy and Beef Manure Applied Soils
… polymerase chain reaction of sul1, tet(W), and 16S rRNA gene, while the microbial community composition was determined via 16S rRNA amplicon sequencing. The results indicated that while prior antibiotic administration elevated the relative abundance of ARGs and changed the microbial community of …
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What Drives Gut Microbial Diversity and Community Structure Among Closely-Related Host Species?
… extracting microbial DNA from fecal samples for 16S rRNA amplicon sequencing. I identified “core” bacterial taxa within herbivore species based on abundance and occurrence within samples. I discovered that certain bacterial taxa serve as environmental and physiological indicators in hosts. I …
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Optimizing Enhanced Biological Phosphorus Removal at WRRFs: Impact of Low DO Operation and Full-Scale Strategies
… at lower DOs, even at higher temperatures, while 16S rRNA amplicon sequencing analysis suggested a community shift toward polyphosphate-accumulating organisms (PAOs). Statistical analysis revealed that low DO operation (DO concentrations below 1 mg O2/L) did not negatively impact effluent TP …
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Characterising Antarctic marine invertebrate microbiomes and their antimicrobial potential
… using a non-culture dependent method (16S rRNA amplicon sequencing). Furthermore, a culture dependent method was used to produce a bank of diverse host-associated bacteria, which were then assessed for their antimicrobial activity, with whole genomes sequenced from the two most …
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Tracking Antibiotic Resistance throughout Agroecosystems
… molecular "indicator"-, and next-generation sequencing- based methods were employed to characterize antibiotic resistance via taxonomic and functional profiles on the broader manure, soil, and vegetable surface microbial communities through 16S rRNA amplicon sequencing and shotgun …
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The Comparative Risk of Antimicrobial Resistance Transfer from Pig, Poultry and Bovine Manure to Grassland
… on grassland microbiomes and resistomes using 16S rRNA amplicon sequencing and high-throughput qPCR (HT-qPCR). Additionally, through culture- dependent approaches the antimicrobial resistance profiles of the WHO priority pathogens Escherichia coli, Klebsiella pneumoniae, Acinetobacter baumannii …
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APPLIED MICROBIAL ECOLOGY OF ANAEROBIC REACTOR MICROBIOMES
… Co-occurrence networks of species based on 16S rRNA amplicon sequences and associations with process parameters were analysed to infer potential metabolic functions and microbial interactions. The results suggested that the process included diverse functions of xylan hydrolysis, xylose …
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Characterising the microbial communities associated with the water distribution system of a broiler farm and their role in Campylobacter infection
… in Campylobacter ecology in the chicken farm. 16S and 18S rRNA profiling of bulk water and biofilm samples were carried out over a seven-week production cycle period. The same samples were screened for the presence of Campylobacter by culture-dependent methods and molecular techniques. Analysis …
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Potential of faeces for non-invasive assessment of Juan Fernandez fur seals and their environment
… be studied from faecal samples. First, I used 16S rRNA amplicon sequencing to characterise the faecal microbiome (Chapter 3). This first faecal microbiome characterisation evidenced a clear separation of the samples into two clusters. Due to the little information available on the species, it …
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Predatory and Mutualistic Interactions between Freshwater Minnows and their Predators
Keystone species are widely distributed across aquatic and terrestrial ecosystems and are fundamental in preserving the structure, diversity, and stability of an ecological community due to its disproportionately large impact on its community relative to its biomass. As biodiversity of ecosystems …
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Role of Water Chemistry in Lead Release during Drinking Water Supply and Augmentation
… samples were collected for DNA extraction and 16S rRNA amplicon sequencing during conditioning and blending. Introducing 100% ATW resulted in a dramatic increase in lead release and simultaneous decrease in copper release from the copper pipes with lead solder. The lead release was driven by …
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A novel semi-passive process for sulphate removal and elemental sulphur recovery centred on a hybrid linear flow channel reactor
… into the microbial ecology was performed using 16S rRNA amplicon sequencing. This enabled the community structure and the relative abundance of key microbial genera to be resolved. These results were used to examine the link between process kinetics and the community dynamics as a function of …