Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 117 for “"16S rDNA"”.
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Identification of The Causative Bacteria In Musculoskeletal Infections Using 16S Rdna - Denaturing Gradient Gel Electrophoresis Analysis
… was to use PCR amplification of a portion of the 16S rRNA gene to test an alternative approach for the identification of these pathogens and to assess the diversity of the bacteria involved. The advantages of this alternative method are that it should increase sample sensitivity and the speed of …
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Molekularbiologische Analyse mikrobieller Gemeinschaften in Talsperrensedimenten
… Arbeit verwendeten Methoden (Vergleichende 16S rDNA Analyse, Fingerprinttechniken, klassische Methoden) konnte eine Charakterisierung der mikrobiellen Zusammensetzung der obersten 5 cm von den Talsperrensedimenten Neunzehnhain, Muldenberg, Quitzdorf und Saidenbach erzielt werden. Die …
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Caracterização e análise filogenética molecular de novos isolados fixadores de nitrogênio
… na homologia do gene para o RN A ribossomal de 16S (16S rDNA) e região intergênica dos genes para os RNAs ribossomais de 16S e 23S (região intergênica 16S-23S rDNA) foi realizada no presente trabalho a partir das técnicas de Polimorfismo de Fragmentos de Comprimento Aleatório amplificados …
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Spartial distribution of cyanobacterial phylotypes in Antarctic Dry Valley soil biotopes
… based approaches. Snap-shot techniques such as 16S rDNA library construction and DGGE have allowed for increased access and better understanding of cyanobacterial diversity from diverse biotopes. In this investigation, 16s rDNA analysis and DGGE profiling were used to study the diversity of …
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Molekulare Differenzierung von Mykobakterien
… Der Informationsgehalt des 5’-Endes des 16S-rRNA-Gens ist ausreichend für die Identifizierung der meisten bakteriellen Spezies. Wegen der vielen fehlerhaften Datenbestände können öffentliche Sequenzdatenbanken die benötigten Referenzsequenzen jedoch nicht zur Verfügung stellen. Es wurde …
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Comparative Serological and Molecular Analysis of Group VIII <em>Spiroplasma</em> from Australia and North America
… by serology. Recent work has generated 16S rDNA sequences that generally correlate with the serological findings. Although the serology and the 16S rDNA sequence analysis clearly classifies strains to the group level, they do not distinguish between strains within the same group. The …
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Molecular characterisation of the fibre-digesting bacteria isolated from the ostrich (Struthio Camelus var. Domesticus) hindgut
… with the aid of PCR amplification of the 16S rDNA genes and sequence analysis using the BLASTN search of the GenBank database. The nucleotide sequences were determined either through direct sequencing of the PCR products or after cloning the products into the plasmid vector pBluescript …
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Life cycle evolution and systematics of Campanulariid hydrozoans
… Chapter 2 presents a phylogeny based on 18S rDNA, calmodulin, 16S rDNA and cytochrome c oxidase I (COI). Ancestral life cycles are reconstructed using parsimony. Medusa loss is common, and Obelia may derive from ancestors with typical medusae. Taxonomic results are discussed in Chapter 3. …
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Analysis of Bacterial Abundance and Species Diversity in Various Soils
… count assay and the microbial abundance by using 16S rDNA to sequence isolated species and compare the number of genera present in each soil type. A qualitative nutrient analysis was conducted to evaluate the relative nutrient content of each soil type. It was determined that the compost was …
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Detection of periodontal bacteria in atherosclerotic plaque
… (PGN). Targeted PCR amplification of the 16S rRNA gene permitted identification of a diverse collection of 16S rDNA sequences within atherosclerotic plaque samples. Thirteen of 21 (61.9%) plaques contained bacterial 16S rDNA relating to periodontal (P. gingivalis and T. forsythia), oral …
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Comparative analysis of primate hydrogenotrophic microbiota
… targeting Archaea and Desulfovibrionales 16S rDNA, clone library construction of Archaea 16S rDNA and mcrA gene, and quantitative real-time PCR targeting mcrA and dsrA genes. Functionality of methanogens was determined by detection of methane from in vitro incubation of fresh feces. The …
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Molekulare Identifizierung von Neisseriaceae und Moraxellaceae mittels ribosomaler DNA-Sequenzierung
… die Sequenzierung partieller Abschnitte der 16S- oder 23S-rDNA können Bakterien meist exakt spezifiziert werden. Hauptziel der vorliegenden Arbeit war es, hypervariable rDNA Abschnitte zu finden, die von stark konservierten Regionen flankiert werden, um auf molekularer Ebene Mitglieder der …
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Microbial Ecology of the Porcine Gastrointestinal Tract in Relation to Lactobacillus Reuteri MM53 and Bacitracin Treatments
… in the diet caused a simplification of V3-16S rDNA banding patterns and subsequently altered sample time phase alignments, which were observed to be sequential in control animals. Introduction of L. reuteri did not significantly alter the total microbial populations of the GIT, however …
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Tick-borne haemoparasite occurrence and Anaplasma bovis strain diversity in eastern rock sengis (Elephantulus myurus)
… are currently limited to the highly conserved 16S rRNA gene and studies are constrained by difficulties with cultivation of tick-borne Anaplasmataceae. The aim of the current study was, therefore, to determine the tick-borne haemoparasite diversity in rock sengi and to expand molecular …
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Genomics Approaches to Improve the Recovery of Bacterial Pathogens in Foods
… compositions in food enrichments through 16s rDNA sequencing, is explored to gain a deeper understanding of pathogen growth relative to the background microbiota of food samples. The first case exemplifies a custom (strain-specific) selective enrichment approach for Shigella recovery from …
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A study of chemolithoautotrophic bacterial rhodanese, and its potential contribution to cyanide wastage during cyanidation of biooxidized concentrates
… the biooxidation pilot plant using the PCR based 16S rDNA profiling technique of Rawlings (1995), and the identification confirmed by 16S rDNA sequencing. Therefore, T. caldus MNG is considered the major contributor to the rhodanese activity of the biooxidation pilot plant mixed culture. Within …
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Detektion und Charakterisierung Polyphosphatspeichernder Bakterien in Gewässersedimenten
… unterschiedlichem phylogenetischem Niveau durch 16S rDNA Klonbibliotheken, DGGE und CARD-FISH. Ergänzende Untersuchungen wurden mittels Inkubationsexperimenten durchgeführt. Es zeigte sich in allen Proben eine große Diversität, in keinem Fall koinzidierte jedoch ein gehäuftes Vorkommen einer …
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Heterotrophe Bakteriengemeinschaften des Deutschen Wattenmeeres: Diversität, Dynamik und Abundanz
… Kompartimente und Stämme mit geringer 16S rDNA Ähnlichkeit mit validiert beschriebenen Spezies aus den Datenbanken (=90%). Drei neue für das Wattenmeer charakteristische phylogenetische Cluster wurden definiert.
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Factors influencing the abundance, community composition and activity states of bacterioplankton from the tidal freshwater James River
… composition was similar based on TRFLP and 16S rDNA pyrosequencing. Community similarity was lower among rRNA libraries suggesting that variable activity states are prevalent in natural communities. Rare taxa were more likely to be metabolically active and were capable of dramatic growth …
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Substrate Specificity and Molecular Characterization of PAH-Degrading Anaerobic Enrichment Cultures Under Methanogenic Conditions
… Characterization of the enrichment cultures by 16S rDNA analysis revealed two unique populations, though they were initiated from the same sediment source. This study set out to determine the ability of these highly enriched communities to utilize a new carbon source, and to identify the effect …
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