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Showing 1 to 20 of 755 for “"16S"”.
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16S rRNA sequence analysis of silver carp microbial community in eDNA water samples
… For this, DNA was extracted and analyzed through 16S rRNA sequencing on the Illumina MiSeq platform yielding 25 million reads and over 16,000 OTUs after initial clean up. Following analysis, there were multiple microbial groups, in particular 127 OTUs within the Fusobacteria phylum previously …
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Assembly of 30s Ribosomes of Escherichia Coli: Properties of Assembly With Precursor-16s-Rna
Made available in DSpace on 2014-12-11T18:16:56Z (GMT). No. of bitstreams: 1 7412253.pdf: 5697902 bytes, checksum: 183d6d6428858437b2a72c3a126a3749 (MD5) Previous issue date: 1973
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Characterization of the structure and function of a <I>Bacteroides thetaiotaomicron</I> 16S rRNA promoter
… a strong housekeeping <I>B. thetaiotaomicron</I> 16S rRNA promoter was examined and compared to an <I>E. coli</I> 16S rRNA promoter. Our hypothesis is that there are significant differences between the promoters of these two organisms. Analysis of <I>B. thetaiotaomicron</I> sequence upstream of …
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Identification of The Causative Bacteria In Musculoskeletal Infections Using 16S Rdna - Denaturing Gradient Gel Electrophoresis Analysis
… was to use PCR amplification of a portion of the 16S rRNA gene to test an alternative approach for the identification of these pathogens and to assess the diversity of the bacteria involved. The advantages of this alternative method are that it should increase sample sensitivity and the speed of …
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The 16S rRNA characterization of a novel "microaerophilic" Pseudomonas sp. from the oligotrophic deep subsurface environment
… the Savannah River DOE site and identified using 16S rDNA sequencing and DNA-DNA reassociation. Micro aerophiles from the Middendorf formation were isolated by use of a semi-solid agar assay, and constituted 10% of the plateable microorganisms. Genetic identification involved the isolation of …
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ANALYSIS OF MICROBIOME IN VARIOUS HUMAN MEDICAL CONDITIONS USING NEXT-GENERATION SEQUENCING OF THE 16S rRNA GENE
… in 43 healthy and diseased patients using 16S rRNA gene sequencing. We identified a rich microbial landscape, identifying over 338 bacterial taxa. Notably, <em>Acinetobacter</em>, <em>Cutibacterium</em>, <em>Acidovorax</em> and <em>Herbaspirillum</em> were prevalent in the ocular samples …
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BIOINFORMATIC PIPELINES AND MICROBIAL DATABASES FOR THE ANALYSIS OF DAIRY COWS 16S RRNA-GENE SEQUENCING DATA: A BENCHMARKING STUDY.
… risultati utilizzando tre diversi database - 16S-ITGDB, RPD, SILVA e Greengenes -, di cui due in due differenti aggiornamenti (SILVA versione 132 e versione 138, Greengenes versione 13.9 e versione 2). Le prestazioni di queste combinazioni saranno testate utilizzando due comunità fittizie e …
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Resolving a Cryptic Species Complex: Application and Utility of Mitochondrial 16S and Nuclear Its1 Molecular Markers in Pyrgulopsis Micrococcus
… analyses based on the partial mitochondrial 16S ribosomal DNA region (16S) and the complete first nuclear internal transcribed spacer region (ITS1) were performed for multiple individuals from each of thirteen populations of P. micrococcus, ten other Pyrgulopsis species, and an outgroup …
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Phylogenetic Relationships of Tick Species in the <em>Ixodes ricinus</em> Complex, Derived from Mitochondrial Coi and 16S Genes
… subunit I (mtDNA COI), and mitochondrial (mt) 16S genes. Ten species from the complex were analyzed; a 504bp fragment was amplified from the mt 16s gene and a 1080pb fragment from mtDNA COI gene. The inferred phylogeny showed that members of the <em>Ixodes</em> <em>rininus</em> complex are …
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Tetracyclin-Resistenz in Helicobacter pylori : Herstellung und Charakterisierung von definierten 16S rRNA-Mutanten und Etablierung eines Realtime-PCR Ansatzes zur schnellen Resistenzbestimmung
… Mutation in der Tetracyclin-Bindungstelle der 16S rRNA zu sein. Bei resistenten H. pylori-Stämmen wurde in beiden Kopien der 16S rRNA-Gene eine Dreifachmutation von AGA 926-928 zu TTC gefunden. <br>Mit dieser Arbeit sollte untersucht werden, ob alle drei Substitutionen für die Resistenz …
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Investigation of the Phylogenetic Relationship of Sporosarcina Ureae to Members of the Bacillaceae Using Primary Structural Characterization of 16s Ribosomal Ribonucleic Acids
Made available in DSpace on 2014-12-14T07:14:42Z (GMT). No. of bitstreams: 1 7616178.pdf: 3880600 bytes, checksum: 0c282ccd25687b115e7bf285bf5a0a0a (MD5) Previous issue date: 1976
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Development of a 16S rRNA gene sequence as a biomarker specific to the gastrointestinal tract and feces of swine based on suppressive subtractive hybridization
… DNA extraction was performed followed by 16S rRNA amplification. The amplified DNA was taken into suppressive subtractive hybridization (SSH) to enrich for swine specific sequences. The final products obtained in SSH were cloned and sequenced. Swine-specific primers were designed and …
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Nmr Solution Structures Of Two Hairpins Of E. Coli 16s Rrna: The Effects Of Mutations And Chemical Modifications On Structure And Function Of Rrna
… A695C, U697A) of the 690 hairpin of E. coli 16S ribosomal RNA were determined by NMR. The UC mutant and the QM mutant with high biological function are able to fold into structures that are isomorphous with the wild-type 690 hairpin sequence. The structural comparisons among the functional …
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T-RFLP analysis of bacterial 16S rRNA gene sequences isolated from river otter (Lontra canadensis) scat and parasite screening for the presence of Toxoplasma gondii
… with the MOBIO PowerSoil™ DNA Isolation Kit and 16S rRNA gene sequences were amplified from each sample. After digestion with Mspl, TRFLPs were analyzed in an ABI Prism™ 310 Genetic Analyzetin triplicate and data peak information from each electropherogram was uploaded into the Phylogenetic …
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SCREENING FOR POTENTIAL ANTIBACTERIAL AGENTS AND THE APPLICATION OF ISOTOPIC LABELS FOR THE CHARACTERIZATION OF THEIR EFFECTS ON THE DYNAMICS OF THE BACTERIAL 16S RIBOSOMAL RNA
… a 27-nucleotide (nt) construct of the bacterial 16S ribosomal RNA (rRNA). We show that, at high concentration, these compounds interfere with bacterial growth. We also observed that the use of efflux pump inhibitors in combination with the fragments enhanced their antibacterial properties and may …
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Comparison of Subterranean Termite (Rhinotermitidae: Reticulitermes) Gut Bacterial Diversity Within and Between Colonies and to Other Termite Species Using Molecular Techniques (ARDRA and 16S rRNA Gene Sequencing)
… amplified rDNA restriction analysis (ARDRA) and 16S rRNA gene sequencing. It was determined that ARDRA was an effective technique for characterizing the diversity of the termite gut microbiota. Of the 512 clones analyzed in the ARDRA study, 261 different ARDRA profiles were found. Forty-two 16S …
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X-Ray crystal structure of a ribosomal protein S6, S15, S18 : rRNA complex from T. thermophilus, and investigation of conformational changes in 16S rRNA fragments from B. stearothermophilus during ribonucleoprotein assembly
The X-ray crystallographic structure of a fragment of the 30S ribosomal subunit from Thermus thermophilus containing ribosomal proteins S6, S15, S18, and a minimal rRNA binding site (T4 RNP) containing two different three-helix junctions was solved to 2.6 A. The protein S15 contains four bundled …
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Caracterização e análise filogenética molecular de novos isolados fixadores de nitrogênio
… na homologia do gene para o RN A ribossomal de 16S (16S rDNA) e região intergênica dos genes para os RNAs ribossomais de 16S e 23S (região intergênica 16S-23S rDNA) foi realizada no presente trabalho a partir das técnicas de Polimorfismo de Fragmentos de Comprimento Aleatório amplificados …
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