Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"143B"”.
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SENSITIZATION OF HUMAN OSTEOSARCOMA CELL LINE 143B WITH CALCITRIOL FOR CISPLATIN THERAPY
… agent in human osteosarcoma cell lines 143B and SaOS-2. The dose response of cisplatin with and without calcitriol (100nM) pretreatment was studied in osteosarcoma cell lines 143B-MM, 143-P and control murine osteoblast MC3T3-E1. Cell proliferation and sensitization effects were …
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Development of the chick chorioallantoic membrane (CAM) xenograft model to study metastasis and angiogenesis in autophagy deficient osteosarcoma cells
… successfully formed tumours from metastatic HOS-143B cells wild type (WT), scrambled control (NC), and CRISPR/Cas9 knockout (KO) ATG7 (ATG7 is an essential regulator of the autophagy process). The role of autophagy in the in ovo and in vitro environment was shown to be context-dependent, with …
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Inhibition of the autophagy pathway modulates osteosarcoma chemoresistance
… chemoresistance. Activation of autophagy in HOS-143B cells following chemotherapy was confirmed by an increased autophagic flux and autophagic clearance of p62. Autophagy-related microRNA (miRNA) expression was found to be decreased in chemotherapy-treated HOS-143B cells. To investigate the role …
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Establishment and characterisation of chemoresistant osteosarcoma cell lines by single and multi-agent induced strategies
… were developed from the cell lines MG-63 and HOS-143B. One of the strategies used the current study includes a multiple drugs combination approach, where cisplatin, doxorubicin, and methotrexate are combined in one treatment. The purpose of this method is to simulate a similar experience with …
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Delineating the metastatic process in osteosarcoma; the role of bone cells, the cell environment and autophagy
… that the highly metastatic OS cell line HOS-143B contains a subset of highly migratory spheroids with enhanced expression of stemness and metastasis-associated genes OCT3/4 (10-fold (p≤0.001)), NANOG (5-fold; p≤0.001), SOX2 (6-fold; p≤0.001), and CXCR4 (2-fold; p≤0.001) compared to monolayer …
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Development of the chick chorioallantoic membrane (CAM) model to assess tumour growth and drug response in osteosarcoma and bladder cancer
… SCaBER) alongside an osteosarcoma cell line (HOS-143B) using the CAM model. Additionally, BC cell behaviour was assessed in a bone-like environment, simulated using a NuOSS scaffold, and compared to a non-bone-like extracellular matrix. Furthermore, a STAT3 inhibitor (STAT3-IN-1) will be applied …
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CRISPR/Cas9-mediated ATG7 knockout results in enhanced osteosarcoma chemosensitivity
… a key gene in the autophagy process, in HOS-143B (highly metastatic/aggressive) osteosarcoma cells. Successful knockout of ATG7, and the autophagy process, was validated by qPCR and cell viability assays. Furthermore, a colony-forming assay was used to examine the effect of ATG7 KO on cell …
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Viral HSV1-TK gene, radiolabeled FIAU, and ganciclovir: combined gene targeted radiotherapy and suicide gene therapy for prostate cancer
… available cell line derived from osteosarcoma (143B-TK) along with its TK-negative clone were also used for comparison. The viral tk gene transfection efficiency was assessed by three independent methods. Firstly, the uptake of [123I]FIAU normalised to the uptake of tritiated thymidine …
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The role of cancer stem cells in osteosarcoma
… OS tumour growth. Eight OS cell lines (143B, Cal72, G292, HOS, MG63, MNNG-HOS, U2OS and SaOS-2) along with the breast cancer cell line MCF7 have been analysed for the presence of sub-population of cells expressing putative CSC markers (aldehyde dehydrogenase and CD117). The intracellular …
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Overcoming chemoresistance in osteosarcoma: the role of autophagy in cell death or survival following chemotherapy
… effect seen on the highly metastatic HOS-143B cells. Therefore, chemotherapy-induced autophagy is predominantly a survival strategy in metastatic OS, although further studies are required to understand the underlying mechanism.
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Characterisation of STRO-1 expression on human mesenchymal stem cells and identification of putative cancer stem cells in osteosarcoma: prevention by micronutrients
… microarray analysis of osteosarcoma cell lines (143B, CAL72, G-292, HOS, MG-63, Saos-2 and U-2-OS) was implemented and a list of eight potential candidate genes encoding STRO-1 antigen was selected. This work ultimately led to the identification of putative cancer stem cells (CSCs) in seven …
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Driving tissue morphogenetic cascades using tunable nanolayered surface coatings
… potent toxicity. Active targeting of 143B osteosarcoma xenografts in NCR nude mice prolonged tumor accumulation by over 7 days. Tumor burden reduced, and in some cases the entire tumor mass was eliminated within 40 days, with a 3 dose regimen. Collectively, this work provides insight …
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Studies on assembly and genetic variation in mitochondrial respiratory complex I
Complex I (NADH:ubiquinone oxidoreductase) couples electron transfer to proton translocation across the inner mitochondrial membrane, to drive the synthesis of ATP. Its distinctive L-shaped structure comprises 45 subunits, encoded by both the mitochondrial and nuclear genomes, which are assembled …