Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"14-3-3 protein"”.
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Crosstalk of protein phosphorylation and lysine acetylation on 14-3-3 proteins and their applications in brassinosteroid signaling and photosynthesis
Protein phosphorylation and lysine acetylation are two important post-translational modifications (PTMs) in eukaryotes. However, the crosstalk between these two modifications is largely unknown. We demonstrated that both phosphorylation and lysine acetylation occurred to an Arabidopsis protein, …
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Caged phosphopeptides and phosphoproteins : agents in unraveling complex biological pathways
Within cellular signaling, protein phosphorylation is the post-translational modification most frequently used to regulate protein activity. Protein kinases and phosphoprotein phosphatases generate and terminate these phosphoryl signals, respectively. Chemical approaches for studying protein …
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A systems approach to uncovering the adaptive response of cancer to targeted therapies
… pull down technique for mass spectrometry using 14-3-3 protein against phosphoserine and phosphothreonine peptides is described. The results of these studies open up many potential avenues for further exploration into the immediate and long-term signaling response of cancer to targeted therapies.
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Intrinsically disordered proteins in molecular recognition and structural proteomics
Intrinsically disordered proteins (IDPs) are abundant in nature, being more prevalent in the proteomes of eukaryotes than those of bacteria or archaea. As introduced in Chapter I, these proteins, or portions of these proteins, lack stable equilibrium structures and instead have dynamic …
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The Study of Wnt Signaling Effector POP-1/TCF in C. Elegans Early Embryos
… presented. POP-1 nuclear asymmetry requires a 14-3-3 protein PAR-5 and at least three POP-1 potential phosphorylation sites for the MAPK LIT-1. LIT-1 activity is required for both POP-1/PAR-5 interaction and phosphorylation of at least two of the three potential LIT-1 sites in vivo. Nuclear …
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Characterisation of the molecular complexes that regulate the G2/M checkpoint of the eukaryotic cell cycle
… from baculovirus-infected Sf9 cells. The wee1/14-3-3β complex was also expressed and purified, incorporating either full length wee1 or a truncated version from which the N-terminal domain of wee1 was deleted. Both exhibited wee1 kinase activity, at equivalent levels, p<0.001, with a 2.4 fold …
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Examination of Placenta and Child Saliva Samples Associated with Childhood Allergy Development
… placental genes are indirect measures of altered protein concentrations in the saliva of children with allergies. The aim of this PhD thesis was to identify proteins associated with childhood allergy using two sources of biological tissue: placental tissue and child saliva. The long term …
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Analysis of SMAUG1 protein motifs and interacting partners - linking regulation of phase separation to human disease
RNA-binding proteins (RBPs) are central regulators of gene expression, influencing RNA processing, localisation, translation, and decay. A growing body of evidence shows that many RBPs exert their functions through the formation of dynamic, membrane-less organelles (MLOs) via liquid–liquid phase …
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Functional Characterization of Four Xanthomonas euvesicatoria Type III Effectors
… function via targeting host defense-related proteins, including atypical receptor-like kinase named TARK1 and a 14-3-3 protein to suppress the PAMPs (pathogen-associated molecular patterns) triggered immunity upon Xe colonization of tomato. In this study, we revealed additional virulence …
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Investigation of phototropin blue light receptor function and signalling in arabidopsis
… thesis. The general structure of the phototropin protein comprises a photosensory region at the N-terminal that contains two LOV (light, oxygen and voltage sensing) domains and a C-terminal kinase domain belonging to the large AGC family of protein kinases. The LOV domains form a covalent adduct …
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A Regulatory Role for 14-3-3 Proteins in the Starch Biosynthetic Pathway of Zea mays
… form large phosphorylation-dependent heteromeric protein complexes. A class of eukaryotic proteins called “14-3-3” dimerize and interact with phosphorylated clients to regulate enzymatic activity, localization and protein interactions. A series of bioinformatic and biochemical techniques …