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Showing 1 to 20 of 61 for “"14-3-3"”.
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Protein kinases that phosphorylate 14-3-3 isoforms
14 -3 -3 is an abundant, predominantly phospho-binding protein, intimately involved in the regulation of many diverse signal transduction events including cell cycle regulation, nucleo-cytoplasmic targeting of essential transcription factors and regulation of catecholamine synthesis. The 14 -3 -3 …
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Die Rolle der 14-3-3-Proteine beim Vorwärtstransport von Kaliumkanälen
… zum ER zurückgeführt, während zytosolische 14-3-3-Proteine am Vorwärtstranport zur Plasmamembran beteiligt sind. Im Verlauf dieser Arbeit konnte gezeigt werden, dass 14-3-3-Proteine nicht nur spezifisch Kir6.2-Reporterproteine binden können, sondern dass diese Proteine am Transport des …
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Regulation of human protein kinases and phosphatases by 14-3-3
… and its paralogues FAM122B and FAM122C are 14-3-3-binding phosphoproteins. The binding of 14-3-3 to FAM122A is mediated by phosphorylation of Ser37 and Ser62; the latter being a site that is phosphorylated in response to cAMP-elevating agents. Binding of 14-3-3 does not affect the …
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Regulation of Mammary Gland Development and Tumorigenesis By 14-3-3 Zeta
… during cancer initiation and progression. 14-3-3z is overexpressed in more than 40% of breast cancers and is associated with poor patient prognosis. Therefore, the function of 14-3-3z in cancer and normal mammary gland development was investigated utilizing multiple <em>in vivo</em> and …
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Untersuchungen zur dynamischen Regulation der Interaktion von RGS7 mit dem Adapterprotein 14-3-3
… Eine entscheidende Rolle hierbei spielt 14-3-3, das mit RGS7 phosphorylierungsabhängig interagiert. Für die Promotionsarbeit ergab sich daraus nun die Frage, wann RGS7 an 14-3-3 gebunden ist und welche Mediatoren die Bindung beeinflussen? Von besonderem Interesse war der vermehrt im …
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Purification of protein phosphatases and 14-3-3-binding proteins and their regulation by the cAMP–PKA pathway
… creates binding sites for proteins such as 14-3-3s, which have been shown to dock onto dually-phosphorylated serine/threonine residues on target proteins. In chapter 4, I looked at the regulation of 14-3-3-binding proteins when the cAMP–PKA signalling pathway was activated. Again, I used …
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14-3-3 Zeta Overexpression Serves As A Novel Molecular Switch Turning Tgf-Beta From Tumor Suppressor to Tumor Promoter
… elusive. Here we identify that overexpression of 14-3-3ζ inhibits TGF-β’s cell cytostatic program through destabilizing p53 in non-transformed human mammary epithelial cells. Mechanistically, we found that 14-3-3ζ overexpression leads to 14-3-3σ downregulation, thereby activates PI3K/Akt signaling …
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Crosstalk of protein phosphorylation and lysine acetylation on 14-3-3 proteins and their applications in brassinosteroid signaling and photosynthesis
… acetylation occurred to an Arabidopsis protein, 14-3-3ω. 14-3-3ω is a member of the 14-3-3 family of proteins that have been widely found in plants. 14-3-3s function mainly as phosphoserine/phosphothreonine-binding proteins that can affect the enzymatic activity, subcellular localization or …
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Design of Peptide Ligands for 14-3-3ε, and Exploring the Role of Residue Types in Ligand Recognition by 14-3-3 Proteins
The 14-3-3 family of proteins are implicated in various malignancies. In cutaneous squamous cell carcinoma (cSCC), overexpression of the 14-3-3ε isoform is associated with suppression of apoptosis. Like 14-3-3ε, the protein phosphatase cell division cycle 25 A (CDC25A) is over expressed and …
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Determining the effects of phosphorylation on AFAP-110 function
… revealed that AFAP-110 contains twelve potential 14-3-3 binding sequences. 14-3-3 proteins are a family of small acidic adaptor proteins of dimeric nature that act as molecular scaffolds and/or allosteric regulators in a number of cellular pathways. We have been interested to determine whether …
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Molecular mechanisms of acquired gemcitabine resistance in pancreatic cancer
… to the parental cell line. I found that 14-3-3σ is up-regulated in the drug resistant cell line due to demethylation in its first exon, and the up-regulation of 14-3-3σ gene expression, in turn, contributes to gemcitabine resistance. Intriguingly, I found that demethylation of the 14-3-3σ …
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Proteomic identification of putative biomarkers of neo-adjuvant chemotherapy resistance in luminal (ER+) breast cancer
… samples our research group recently identified 14-3-3 theta/tau, tBID and BcL-XL as putative biomarkers of response to neoadjuvant chemotherapy (Hodgkinson et al J Prot 2012, 75:1276-1283 and 75:2745-2752). Here we aimed to analyse further samples using the AbMA approach and to re-analyse the …
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Methionine Sulfoxide Reductase and Ubiquitin-dependent Degradation of Alpha-synuclein
… was found to bind Ub and the molecular chaperone 14-3-3 proteins, leading to enhanced αSyn aggregation. Our lab recently reported on elevated dopamine and 14-3-3 levels in the methionine sulfoxide reductase A (MsrA) knockout mouse brain, as well as decreased αSyn degradation in MsrA KO yeast …
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The identification of biomarkers of chemotherapy resistance in breast cancer using comparative proteomics
… in at least 2 datasets and these included 14-3-3 theta/tau, BID and Bcl-xL. Three datasets were generated using 2D-PAGE with MALDI-TOF/TOF MS, containing 132 unique DEPs. These included several isoforms of 14-3-3 proteins. The differential expression of 14-3-3, BID and Bcl-xL was confirmed …
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Identification of new genes and pathways that act to delay C. elegans aging
… transcription factor DAF-16. We discovered that 14-3-3 proteins play a role in C. elegans lifespan determination. 14-3-3 proteins interact with the SIR-2.1 protein, and the 14-3-3 genes par-5 andftt-2 are required for the longevity mediated by sir-2. I1 overexpression, indicating that 14-3-3 …
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Role of Phosphorylation In The Regulation of Prmt5
… with several SH2-domain containing proteins, 14-3-3 proteins and the FHA domain of MDC1. These novel phospho-dependent PRMT5 interactions suggest that crosstalk between kinases and arginine methyltransferases may play a pivotal role in modulating the different cellular functions of PRMT5.</p> …
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Analysis of the role of Mdm38 in respiratory chain biogenesis
… Bereich ist auf tertiärer Ebene stark homolog zu 14-3-3 Proteinen und relevant für die Translationsregulation von Cox1 und Cob. Mdm38 kann daher als das erste 14-3-3-ähnliche mitochondriale Protein bezeichnet werden. Der identifizierte Bereich weist zudem Ähnlichkeit mit der vorhergesagten …
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Regulatory And Functional Aspects Of Foxo3a Transcription Factor And Their Implications In Prostate Cancer
… by Akt generates binding sites for 14-3-3, a protein which regulates FoxO3a DNA binding, transcriptional activity, and nuclear localization. Once phosphorylated, FoxO3a accumulates in the cytoplasm where it is subsequently degraded. Our goal is to further elucidate the functional …
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Examination of Placenta and Child Saliva Samples Associated with Childhood Allergy Development
… haptoglobin and complement C3. Moreover, 14-3-3 protein had very high expression in allergic children and very low expression in the non-allergic group. Using child saliva samples, 177 proteins were identified from MaxLFQ analysis. Of these proteins, six candidate proteins with significant …
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