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Showing 1 to 15 of 15 for “"0601 Biochemistry and Cell Biology"”.
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Identification and characterisation of the cell surface and enzymatic barriers to plasmid transformation in Corynebacterium glutamicum and related species
… glutamicum, Brevibacterium flavum, and B. lactofermentum have been used traditionally for industrial production of amino acids. Recently, these strains have been also studied using molecular biology or recombinant DNA approaches. However, a major early limitation in the application …
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A study of rat skeletal muscle Troponin C isoforms
… heavy chain (MHC) isoform based fibre-typing and Sr2+ -activation measurements to explore the relationship between MHC and TnC isoform composition in mammalian skeletal muscle at the single fibre level, and to revisit the controversial issue of the relationship between TnC isoform composition …
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A study of the role of glycogen in skeletal muscle performance and of myosin heavy chain isoform expression in amphibian skeletal muscle using the toad Bufo marinus
… underlying the positive correlation between intracellular glycogen content and skeletal muscle performance and the other with the MHC isoform composition in amphibian skeletal muscle and single muscle fibres. The organism used throughout this study was the cane toad Bufo marinus.
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The effects of feed supplemented with omega-3 polyunsaturated fatty acids on cultured abalone
… the risk of cardiovascular diseases, arthritis and certain cancers. In addition, they have also been found to play a role in reducing the risk of Alzheimer’s disease, depression and schizophrenia. Seafoods are the main sources of LC n-3 PUFA. The LC n-3 PUFA and total lipid contents of marine …
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The texture and microstructure of mozzarella cheese as affected by fat content, EPS producing starter culture and fat replacers
… using fat replacers having similar texture and microstructure characteristics as full fat mozzarella. To achieve this goal it was essential to analyse the texture characteristics including hardness, cohesiveness, springiness, chewiness and gumminess of commercial mozzarella cheeses and to …
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Studies on extracellular polysaccharide-degrading enzymes secreted by Pleurotus ostreatus grown on lupin hull
… resulting from the processing of the lupin seeds and it contains mainly polysaccharides, which is unusable by human and stock directiy. One simple potential approach to utilise this waste is to cultivate mushrooms. Three mushroom species (Pleurotus ostreatus, Lentinus edodes and Flammulina …
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A study of biochemical and physiological properties of normal and functionally impaired skeletal muscles
… aim of this study was to increase our understanding of the diversity and plasticity of skeletal muscle by using (i) normal and functionally impaired rat muscles from normotensive and spontaneously hypertensive rats, (ii) single, skinned fibre preparations, and (iii) a combination of …
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Isolation of low ethanol producing yeast strains using adaptive evolution
… oxidation of NADH. To compensate for this, the cell regenerates NAD by producing increased amounts of glycerol; glycerol production is an NADH-driven reductive process. It was demonstrated in this thesis that culturing yeast in presence of sulfite for a number of generation, results in the …
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Blonde hair colour: classification, characterisation and genetic associations for use in forensic science
… pigmentation variation is a huge genetic puzzle and hair colour is one part of this puzzle. It is hypothesised that there are genetic variants specifically associated with an objectively defined Blonde phenotype. The first objective of this study was to examine the natural, adult hair colour …
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Steric aspects of the interaction of metal species with nucleic acid constituents
… have been investigated both from experimental and theoretical viewpoints. Manipulation of steric effects within model systems has been carried out by way of judicious ligand design.
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Prediction of age from DNA
… could use a correlation between telomere length and age, to predict the age of an individual from their DNA. Telomere length, in buccal cells, of 167 individuals aged between 1 and 96 years old was measured using quantitative real time PCR. The causes for the presence of large variation in …
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Production of poly(adenosine diphosphate-ribose) polymerase-1: development of an efficient production and purification protocol
… a key role in maintaining the integrity of DNA and chromatin structure. As such, this enzyme is likely to provide a useful target when using a rationale drug design approach to develop pharmaceutical reagents including cancer therapeutics. A major obstacle to this work however is that our …
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Exercise, electrical stimulation and ionic effects on Na+, K+-ATPase isoform gene and protein expression in mammalian skeletal muscle
This thesis examines the effects of acute and chronic exercise, electrical stimulation and explores factors regulating Na+,K+ -ATPase isoform transcription and translation in skeletal muscle.
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The pharmacokinetics of sodium monofluoroacetate (1080) in rabbits
… (1080) is a pesticide widely used in New Zealand to control rabbits and possums. Very little is known about the in vivo behaviour of 1080 in target and non target species. This experiment was carried out to investigate the persistence and tissue distribution of 1080 in rabbits and assess the …
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Biomarkers in the measurement of hepatic damage in sheep
The measurement of urinary taurine and the liver specific enzymes AST and GGT in sheep with hepatic damage was undertaken to determine if urinary taurine could be used as a biomarker of hepatic damage. Two treatment groups (n = 5) were treated with O.125ml/Kg BW of carbon tetrachloride (CCl₄) …