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Showing 1 to 5 of 5 for “"세포배양"”.

  1. Identification of Neuropeptide FF receptor 2 (NPFFR2) and its ligand NPFF as a factor to affect cancer progression

    Ⅰ. 서론 1 Ⅱ. 재료 및 방법 5 A. 간암 세포주 및 세포배양 5 B. RNA 분리 및 RT-PCR 5 C. 발현 조절 및 펩티드를 이용한 수용체 활성화 6 D. Western blot 6 E. Invasion assay 7 F. Wound healing assay 7 G. Colony formation assay 8 Ⅲ. 결과 9 Ⅳ. 고찰 26 Ⅴ. 결론 30 참고문헌 31 ABSTRACT 38

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  2. Genetic Polymorphism Study of Prostaglandin E2 Receptor Subtype EP4 gene (PTGER4) in Aspirin - Intolerant Urticaria

    … = 5 B. 프로스타글란딘 E2 수용체의 단일염기다형성의 기능적 연구 = 11 1. 세포배양 = 11 2. 역전사 중합효소연쇄반응(RT-­PCR)을 이용한 다양한 세포주에서의 PTGER4 유전자의 발현 = 11 3. Promoter construct 제작과 luciferase repoter assay = 14 4. 전기 이동성 교대 분석 실험 (Electrophortic mobility shift assays, EMSA) = 15 5. 공동세포이입(cotransfection)을 이용한 PTGER4 promoter활성에서의 …

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  3. 세포자멸사 촉진분자를 이용한 암 치료용 단백질치료제의 개발

    … - ⅴi 표 차례 - viii Ⅰ. 서 론 - 1 Ⅱ. 재 료 및 방법 - 6 A. 세포배양 - 6 B. Apoptosis inductcing stimulus - 6 C. Construct - 7 D. 돌연변이 Bcl-xL의 과발현을 위한 construc 구축 - 8 E. Transfection - 9 F. Western blot analysis - 10 G. Fluorescence activated cell sorter(FACS) analysis - 11 H. co-Immunoprecipitation analysis - …

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  4. Development and characterization of recombinant human autoantibody using cDNA made from peripheral blood mononuclear cells of patients with bronchial asthma

    … 제작 및 정제 12 1. IgG 자가항체의 제작 12 2. IgG 자가항체 생산을 위한 세포배양 12 3. Protein A column을 이용한 IgG 자가항체의 정제 13 G. IgG 자가항체의 CK-18 단백질에 대한 반응 평가 13 1. IgG 자가항체를 이용한 항원 반응특성의 ELISA 13 2. Immunoblot 분석 14 3. Immunofluorescence analysis 14 4. Flow cytometry 분석 15 H. IgG 자가항체의 세포독성 평가 및 세포사멸 기전 관찰 15 1. 3- …

    ajou Repository record for Development and characterization of recombinant human autoantibody using cDNA made from peripheral blood mononuclear cells of patients with bronchial asthma (opens in a new tab)

  5. The Studies for the Preparation of Platelet Lysate (PL) and the Effects of Platelet Lysate (PL) on the Proliferations for Microbial and the Human derived cells

    After it became known that the platelets release growth factors, numerous research on the use of platelet rich plasma (PRP) for the purpose of wound healing and regeneration have been reported from various fields in the medical world. The research on PRP gels and platelet lysates (PL), which are …

    ajou Repository record for The Studies for the Preparation of Platelet Lysate (PL) and the Effects of Platelet Lysate (PL) on the Proliferations for Microbial and the Human derived cells (opens in a new tab)