Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"β-strand"”.
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Design and synthesis and testing of conformationally constrained peptidomimetics.
… a bioactive conformation (α-helix, β-turn or β-strand) by incorporation of a ring or bridge is discussed. The technique of ring closing metathesis as a strategy for cyclisation of peptidomimetics is introduced. Chapter Two surveys β-turn mimics comprised of β-amino acids. The synthesis of novel …
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Small Molecules as Amyloid Inhibitors: Molecular Dynamic Simulations with Human Islet Amyloid Polypeptide (IAPP)
… in part, to the formation of inter-peptide β-strands comprised of residues 23-27 (FGAIL). Flavonoids, a class of polyphenolic natural products, have been found experimentally to inhibit IAPP aggregate formation. Many of these known IAPP aggregation attenuating small flavonoids differ …
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Engineering Fluorescently Labeled Human Fibroblast Growth Factor One Mutants and Characterizing Their Photophysics Properties Towards Designing FRET Assays
… and T79C-hFGF1 (on the rigid loop between β strand 7-8). The main findings showed that the dye and protein required a certain degree of flexibility to obtain high labeling efficiency but having too much flexibility caused low labeling efficiency because of entropic penalty during conjugation. …
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Structural and functional studies of intrinsically disordered fibronectin-binding proteins
… of SfbI 5 was shown to have propensity for β-strand conformations and, thus, might contain a preformed contact site involved in the initial interaction between SfbI-5 and 1−5F1. A high resolution structure of an SfbI-5 peptide in complex with 2F13F1 demonstrated that peptide binding occurs …
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Modulation of Immunity via Antibodies Displayed on in situ Forming Peptide Co-assemblies
… peptides implies that EAKIIH6 might adopt a β-strand conformation and align with existing EAK16-II β-sheets during co-assembly. It was also found that the accessibility of His-tags could be tuned by changing the ratio of peptides. To conclude, these results demonstrate the peptide co-assembly …
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Expression of cysteine-rich pig gastric mucin (Muc5AC) domains in Pichia pastoris and their pH-dependent properties
… α-helix content and increased random coil or β-strand for the C-terminal and Cys-repeat peptides respectively. Thus, decreasing pH causes an increase in the exposure of, and binding to, hydrophobic sites of recombinant peptides from the Cys-rich regions of PGM. The increased hydrophobic …
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MECHANISM OF PINK1-MEDIATED UBIQUITIN PHOSPHORYLATION
… that in a small population of Ub the last β-strand is retracted, resulting in a more accessible Ser65 loop. It could be shown that PINK1 binds the Ser65 loop in this C-terminally retracted conformation (Ub-CR), but not in the ‘common’ conformation. In addition, it could be shown that Ub …
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Structural And Functional Studies On Thrombin Allostery
… occluded by a collapse of the entire 215-219 β-strand. Binding of PAR1 fragment to exosite I, 30-Ã… away from the active site region, causes a large conformational change and restores access to the active site, akin to the E form in the D102N mutant but not the WE mutant. Using a network of …
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Nuclear Export Receptor CRM1 Recognizes Nuclear Export Signals with Diverse Conformations
… showed CRM1-bound NESs adopting either helix-strand or mostly extended conformations while the CRM1 groove remains unchanged. The plasticity of CRM1-NES interaction and the repertoire of NES conformations were unclear. Many NES sequences also seem incompatible with the asymmetric and seemingly …
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Understanding the role of TapA in the formation of <i>B. subtilis</i> biofilms: a biochemical and genetic analysis
… highlighting the importance of a potential β-strand secondary structure in the Nterminus of TapA. Evidence is provided that SipW is not required for the ability of exoproteases to process TapA, suggestive that TapA secretion is not SipWdependent. Experimental evidence indicates that TapA could …
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Characterizing unique features of prion-like domains recruited to stress granules
… hydrogen bonds are made along each exposed β-strand. Solid-state assemblies in biomolecular condensates such as the Balbiani Body in oocytes share a similar β-sheet rich structure that contributes to low dynamicity of the interactions in this separated phase. Prion-like domains (PrLDs) are …
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Computational Development for Secondary Structure Detection From Three-Dimensional Images of Cryo-Electron Microscopy
… - <em>SSEtracer</em>, <em>SSElearner</em>, <em>StrandTwister</em> and <em>StrandRoller </em>for solving this critical problem.</p> <p>An effective approach, <em>SSEtracer</em>, is presented to automatically identify helices and β- sheets from the cryo-EM three-dimensional maps at medium …
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The synthesis of bimane constrained peptides and their fluorescent and structural properties
… was deduced that the bimane linker can induce β-strand like structure in an i-i+2 constrained peptide; in contrast an i-i+4 constrained pentapeptide with homocysteine in the 1 and 5 positions results in a 3₁₀ helical like structure. β-alanine containing analogues of these peptides were also …
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The Role of METTL21A in Kras-driven Cancers
… amember of the little-studied seven β- strand family of candidate lysine methyltransferases (KMTs), and its role in regulating pancreatic ductal adenocarcinoma (PDAC) and lung adenocarcinoma (LUAC) tumorigenesis. Through the meta-analysis of publicly available gene expression datasets, I …
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Interaction of Bacteriophage Mu Middle Transcription Activator Protein Mor with Promoter DNA
… of Mor that residues Y70 and Q68</p> <p>of the β-strand whose side chains extend away from the protein, make base</p> <p>specific interactions in the DNA minor groove.</p> <p>2. To identify the bases between -30 and -57 of the promoter Pm that are important</p> <p>for its function, in terms of …
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Mining the enolase superfamily for new functions: investigations of D-glucarate dehydratase related proteins (GlucDRP) and L-lyxonate dehydratase proteins (LyxD)
… His-Asp dyad at the end of the seventh and six β-strands of the (β/α)7β-barrel domain and a KxR motif at the end of second β-strand. This is the first example of an L-lyxonate dehydratase in the enolase superfamily and the first example of a pathway for degradation of L-lyxonate.
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USING B-STRAND AND B-HAIRPIN PEPTIDOMIMETICS INDUCED BY CONSTRAINED AMINO ACIDS TO MODULATE THE AGGREGATION OF THE MICROTUBULE ASSOCIATED PROTEIN TAU: DESIGN, SYNTHESIS AND EVALUATION
Tauopathies refer to neurodegenerative disorders characterized by the presence of abnormal aggregates of Tau protein, leading to the formation of neurofibrillary tangles (NFTs) within neuronal cells. Tau, a protein associated with microtubules (MTs), plays a crucial role in regulating the dynamics …