Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 21 for “"β-glucuronidase"”.
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Separation of Recombinant β-Glucuronidase from Transgenic Tobacco by Aqueous Two-Phase Extraction
… of an acidic model protein, recombinant β-glucuronidase (rGUS), from transgenic tobacco with high yield and purity. Aqueous two-phase extraction (ATPE) is a powerful technique for separation and purification of proteins, and has the potential to replace an expensive chromatography step for …
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Lysosomal Enzymes in Undifferentiated and Differentiated HL-60 Cells
… Elastase, chymotrypsin, ribonuclease, β-glucuronidase and lysozme concentrations were determined in the lysosomal fraction and whole cell lysates. The amount of chymotrypsin and elastase increased significantly in cells which had differentiated to macrophage-like cells. The intrracellular …
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Comparison of Extraction Techniques for Δ-9-Tetrahydrocannabinol from Urine
… research, either a strong alkaline solution or β-glucuronidase has been used to cleave the bond between the Δ-9-THC and glucuronic acid before an alkaline extraction. Δ-9-THC has a pKa of 10.6 and therefore is always extracted at an alkaline pH. In this study, both a strong solution ofNaOH and the …
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Expression and activity of enzymes in the human placenta: pharmacological & toxicological consequences in AZT therapy
… perfusion model into the first trimester. UGT, β-glucuronidase, Cytochrome p4501A (CYPIA) and CYP reductase were present and active in the human placenta. CYP2E1 protein was expressed but not active. CYPIA, CYP2EI and the UGT2B subfamily were expressed across gestation, but the UGTIA subfamily was …
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Conformational Analysis of Disordered Proteins Using H/D Exchange Mass Spectrometry
… H/D-MS study of the conformational dynamics of β-glucuronidase was also conducted. The destabilization of β-glucuronidase that results from the W492G mutation and the partial conformational rescue that results from indole addition were analyzed. A new method to interpret and normalize H/D-MS data …
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The Development of Certain Enzymes and Iso-Enzymes During Normal Pregnancy
… n-acetyl-β-galactosaminidase and β-glucuronidase, have been found to progressively increase. Dipeptidase and tripeptidase were not found to increase during pregnancy. A wide scatter of results was found during each week of pregnancy which was due in part to poor reproducability of …
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Diagnostic use of hair analysis for the detection of misuse of amfetamines and cannabinoids
… and pre-treatment methods, alkaline (1M NaOH), β-Glucuronidase (helix pomatia), methanol (MeOH) and acid (0.1M HCI) were used on these and compared. The best recovery for amphetamine was obtained using the β-glucuronidase pre-treatment method and this extract was also found to be cleaner than the …
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I. Etablierung eines induzierbaren Suizidsystems zur Identifizierung von Mutanten der salizylsäureabhängigen Signaltransduktion II. Expression von tierischen Signaltransduktionskomponenten in Tabak zur Herstellung eines induzierbaren Expressionssystems
… unterscheiden zu können, wurde die β-Glucuronidase unter der Kontrolle des gleichen Promotors als zweites Reportergen eingebracht.Das regulatorische Protein NPR1 ist eine entscheidende Komponente bei der durch Salizylsäure induzierten Aktivierung der späten" Abwehrgene. Durch Kreuzung …
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Factors influencing transient gene expression in electroporated tall fescue (Festuca arundinacea Schreb.) protoplasts
… studied in protoplasts, after the reporter β-glucuronidase (GUS) gene was introduced by electroporation. In a time-course study of transient gene expression, GUS activity peaked at 24 h after electroporation. Among the different field strength conditions tested, maximum GUS activity was …
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Functional metagenomic discovery and characterisation of CAZymes by microfluidic methods
… droplet sorting (FADS) for β-glucuronidase activity leading to the discovery of hit SN243. This enzyme belongs to the glycoside hydrolase (GH) family 3, a family that had no previous record of β-glucuronidase activity at the outset of this study. Detailed functional and …
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Secretion of active recombinant phytase from stably transformed soybean cells
… and bombardment was optimized as assayed by β-glucuronidase reporter gene expression. A somatic embryogenesis approach was used for soybean regeneration from culture. The efficiencies of embryo induction and embryo conversion to form roots and shoots were compared in commercial soybean …
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Variability in fecal microbial drug deglucuronidation
… Several intestinal bacteria produce various β-glucuronidase enzymes, which catalyze deglucuronidation of compounds formed during UDP-glucuronosyltransferase (UGT) mediated phase II metabolism. Deglucuronidation can result in the formation of an inactive, active, and/or toxic compound, which may …
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Molecular and Functional Characterization of Terpene Chemical Defense in Arabidopsis Roots in Interaction with the Herbivore Bradysia spp. (fungus gnat)
… to the root leucoplast. Expression of the β-glucuronidase (GUS) reporter gene fused to the upstream genomic region of AtTPS08 demonstrated constitutive promoter activity in the root vascular tissue and root tips. Using the established bioassay with Arabidopsis and Bradysia larvae, in …
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Bone-Specific Metabolism and Mechanism of Action of Curcuminoids in Blocking Osteolysis in Breast Cancer and Other Resorptive Bone Diseases
… feed-forward loop. However, mouse bone marrow β-glucuronidase (GUSB) deconjugated curcumin-glucuronide to the active aglycone, which was maintained across sexual and skeletal development and with bone resorptive disorders, with evidence that human bone marrow also has deconjugation activity. …
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Pathogenicity of Murine Cytomegalovirus Mutants
… vivo. Recombinant viruses (RV) expressing the β-glucuronidase marker gene were constructed by site-directed insertion and deletion mutagenesis of the MCMV Hind III-J and -I regions of the viral genome. Mutations were targeted to this region of the MCMV genome because the corresponding region of …
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Purification of an acidic recombinant protein from transgenic tobacco
… of the acidic model protein, recombinant β-glucuronidase (rGUS), from transgenic tobacco leaf tissue to high yield and purity. Polyelectrolyte precipitation with polyethyleneimine (PEI) was identified as an initial purification step for purifying acidic recombinant proteins from tobacco. …
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Development and validation of ultra-high performance liquid chromatography-tandem mass spectrometry methods for screening of selective androgen receptor modulators in urine and blood
… human) with and without enzymatic hydrolysis (β-glucuronidase/arylsulfatase) in urine and in blood (equine plasma and bovine serum) were developed. Method validation was performed in all species of interest according to respective EU Commission Decision 2002/657/EC criteria and European Union …
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Untersuchungen zur Metallhomöostase in Arabidopsis thaliana
… wurde mit Pflanzen durchgeführt, die das β-Glucuronidase (GUS)-Reporterprotein bzw. chimäre Fusionsproteine aus EGFP und AtMTP2 unter Kontrolle des nativen pMTP2-Promotors exprimierten. Diese Experimente bestätigten zum einen, dass der pMTP2-Promotor nur unter Zn-Defizienz aktiv ist. …
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Investigations into the Mechanisms that Are Responsible for Reduction in Colonic SN-38 Exposure
… of SN-38 glucuronide to SN-38 by bacterial β-glucuronidase secreted by commensal microbiota, generation of toxic active metabolite SN-38 from irinotecan mediated by intestinal carboxylesterse enzymes in gut and enterohepatic recycling of irinotecan and SN-38, etc., can contribute to the …
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Functional genomics of plant compensatory responses
… fusion studies (G6PDH1 promoter: β-glucuronidase (GUS)) and subsequent histochemical staining revealed that G6PDH1 is expressed in virtually all tissues rather than localized to any specific tissue. These results are consistent with patterns of regrowth observed following clipping in …
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