Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 41 for “"β-glucosidase"”.
-
Purification and characterization of fungal β-glucosidase enzymes
… and one of the key enzyme group is β-glucosidases. These enzymatic systems are mainly adopted from wood-decaying fungi. The overall enzymatic system consists of different types of cellulases that first degrade the crystalline cellulose to oligosaccharides and cellobiose. In the final …
-
Maize β-glucosidase substrate specificity and natural substrates
Plant β-glucosidases (β-D-glucoside glucohydrolases, E.C. 3.2.1.21) are known to function in defense in cyanogenic systems, and may also function in the metabolism of phytohormone glucosides and glucosides of other secondary plant products. Maize (Zea mays L.) β-glucosidase is a homodimer of 60 KD …
-
Determination of the Binding Site and the Key Amino Acids on Maize β-Glucosidase Isozyme Glu1 Involved in Binding to β-Glucosidase Aggregating Factor (BGAF)
β-Glucosidase zymograms of certain maize genotypes (nulls) do not show any activity bands after electrophoresis. We have shown that a chimeric lectin called β-glucosidase aggregating factor (BGAF) is responsible for the absence of β-glucosidase activity bands on zymograms. BGAF specifically binds …
-
The effects of the expression levels of cellobiose transporter and β-Glucosidase on ethanol fermentation
… enzymes such as cellobiose transporter and β-glucosidase, the ratios between those two enzymes can be a significant factor on cellobiose fermentation. In order to assess the effects of expression level of cellobiose transporter (CDT) and β-glucosidase (β-GL), in this study the copy number …
-
Cloning and sequencing of a β-glucosidase of cDNA from Sorghum bicolor (L.) Moench and analysis of expression in seedlings
A full-length β-glucosidase encoding cDNA is isolated and sequenced from Sorghum bicolor (L) Moench. Using 5’- and 3’- end specific probes derived from the cDNA clone, the multiplicity of β-glucosidase genes and their expression in different tissues were studied. Southern blotting data showed that …
-
Characterization of a Beta-glucosidase Aggregating Factor Responsible for the Null Beta-glucosidase Phenotype in Maize (Zea mays L.)
β-Glucosidase (β-D-glucoside glucohydrolase, EC 3.2.1.21) catalyzes the hydrolysis of aryl and alkyl β-D-glucosides as well as glucosides with a carbohydrate moiety such as cellobiose and other beta-linked oligosaccharides. In maize (Zea mays L.), β-glucosidase exists as 120 kD homodimers, but also …
-
Isolation and characterization of rice (Oryza sativa L.) β-Glucosidases
… purification and characterization of rice β-glucosidase, (2) determination of the physiological role of the enzyme during rice germination, and (3) histochemical localization of the enzyme. The method for partial purification of the enzyme was based on that of Schliemann (1984), which …
-
Stability of bioactive isoflavones and glycolytic enzymes produced by probiotic bacteria in soy based food during processing and storage
… storage in soymilk has not been studied. β-glucosidase is an important enzyme that could be used in the bioconversion of the predominant soy isoflavone glycosides to their bioactive aglycone forms. Fifteen probiotic micro-organisms that included Bifidobacterium sp., Lactobacillus acidophilus …
-
Enhancing the transformation level of bioactive soy isoflavones in soy-based foods by probiotic organisms
… IG to IA. Initially, pure β-galactosidase and β-glucosidase were utilised for hydrolysing IG to IA in soymilk (SM). The level of hydrolysis ranged from 43.3-77.2% and 86.7-93.0% by various β-galactosidase concentrations and β-glucosidase, respectively. Six strains of probiotic organisms that …
-
Purification and characterisation of novel recombinant β-glucosidases from aspergillus with application in biofuel production
β-glucosidases are important components of the cellulase enzyme system in which they not only hydrolyse cellobiose to glucose, but also remove the feedback inhibition effects of cellobiose on exoglucanase and endoglucanase thereby increasing the rate of cellulose degradation to fermentable sugars. …
-
Mechanism of Substrate Specificity and Catalysis in Retaining β-Glucosidases From Maize and Sorghum
β-glucosidases catalyze the hydrolysis of aryl and alkyl β-D-glucosides as well as glucosides with a carbohydrate moiety. The maize β-glucosidase isozymes Glu1and Glu2 hydrolyze a broad spectrum of substrates in addition to its natural substrate DIMBOAGlc, while the sorghum β-glucosidase Dhr1 …
-
Dissecting the jasmonate singalling pathway in Arabidopsis thaliana
… by methyl jasmonate. A cDNA encoding a β-glucosidase, PYK10, was previously isolated using the differential display technique in an attempt to isolate methyl jasmonate-induced genes in Arabidopsis thaliana. β-glucosidases catalyse the breakdown of glucosinolates into glucose and sulphate …
-
The Utilization of Dietary Carbohydrates by Trout and Carp
… or persistent hyperglycaemia. α-amylase and β-glucosidase activities increase initially with increase in dietary dextrin, and is higher in Carp than in Trout. 45% dextrin in the diets of carp produced the highest levels of α-amylase in the liver and β-glucosidase in the hind-gut. Carcass and …
-
Effects of AFEX Pretreatment, Denisifcation and Storage on Ethanol Yields of Corn Stover, Switchgrass and Prairie Cordgrass
… Two enzyme doses of cellulase and β-glucosidase were employed, using a separate hydrolysis and fermentation (SHF) system. The high dose utilized 15 filter paper units (FPU)/g glucan of cellulase and 64 cellobiase units (CBU)/ml β-glucosidase. The low dose rate used one-third of each …
-
Response of Soil Properties to 23-25 Years of Diverse Crop Rotations and Tillage Systems in South Dakota, USA
… 1M and 6M], soil urease enzyme, and soil β-glucosidase. Data of soil quality showed that after 23 years, the 4-yr rotation significantly increased SOC concentrations up to 30-cm in both tillage systems. Averaged across all the tillage systems, the 4-yr rotation increased SOC by 10.6 and …
-
Purification and characterization of an endo-1,4-{u03B2}-D-glucanase and two exo-1,4-{u03B2}-D-glucanases from the cellulase system of Trichoderma reesei
… than forms of the same enzyme. An enriched β-glucosidase preparation from the culture filtrate of Trichoderma reesei QM 9414 formed a single precipitin line when allowed to react with antiserum to the β-glucosidase previously purified from a commercial T. viride cellulase preparation. The …
-
Pathway optimization and engineering for biofuel production
… describe the simultaneous optimization of the β-glucosidase (gh1-1) and the cellodextrin transporter (cdt-1) through directed evolution of the pathway. The improved pathway was assessed based on specific growth rate on cellobiose, with the final mutant exhibiting a 42% increase over the wild-type …
-
Nutritional capability of and substrate suitability for Pseudogymnoascus destructans, the causal agent of bat white-nose syndrome
… Enzyme assays implicate that both urease and β-glucosidase appear to be constitutive, lipase and esterase activity were more rapid than proteinase activity on 6% gelatin, gelatin degradation was accompanied by medium alkalinization, the reduction of thiosulfate generated hydrogen sulfide gas, …
-
Improving advanced biofuels production in Saccharomyces cerevisiae via protein engineering and synthetic biology approaches
… consisting of a cellodextrin transporter and a β-glucosidase was introduced into S. cerevisiae to allow co-fermentation of mixed sugars. However, the utilization of cellobiose was still much lower than that of glucose, and the uptake of cellobiose was considered the rate-limiting step for …
Page 1 of 3