Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 60 for “"β-galactosidase"”.
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Towards novel luminescent probes for monitoring β- galactosidase activity
… in vivo the processing of sugars by β-galactosidases. To this end, a lactose-based probe was designed, featuring a Lanthanide held within a chelate and appended to the glucosyl unit, and a proximal naphthyl moiety, attached to the galactose unit, which would function as a sensitiser for …
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Development of a high throughput reporter system using β-Galactosidase in the yeast : Pichia Pastoris
… the lacZ reporter gene, which encodes for the β-galactosidase protein, and fused to the S. cerevisiae MATa signal sequence. Expression plasmids were successfully transformed in P. pastoris strain yGS 115 followed by induction. Western blot analyses confirm the expression of β-galactosidase and …
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Investigating the effects of calreticulin and beta-galactosidase on microglial functions and neuronal loss
… loss were affected by calreticulin and β-galactosidase, two proteins associated with ageing, chronic inflammation, and neurodegeneration. The aggregation of amyloid-β to form oligomers and insoluble amyloid plaques in the brain is a hallmark of AD. Amyloid-β can be directly neurotoxic and …
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PROTEIN PURIFICATION FROM GASTROPODS
… β-mannosidase, α-glucosidase, β-glucosidase, β- galactosidase and β-N-acetylglucosaminidase) in 8 different species of snails (Arion lusitanicus, Biomphalaria glabrata, Achatina fulica, Limax maximus, Cepaea hortensis, Lymnea stagnalis, Arianta arbustorum, Planorbarius corneus and egg from …
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Calcium homeostasis in Pichia pastoris
… the expression of alcohol oxidase-1 driven β-galactosidase expression. However, calcium and magnesium may still play crucial roles in protein expression as EDTA and EGTA caused an increase in β-galactosidase expression as indicated by higher β-galactosidase activity. Our findings suggest that …
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Enhancing the transformation level of bioactive soy isoflavones in soy-based foods by probiotic organisms
… to transform IG to IA. Initially, pure β-galactosidase and β-glucosidase were utilised for hydrolysing IG to IA in soymilk (SM). The level of hydrolysis ranged from 43.3-77.2% and 86.7-93.0% by various β-galactosidase concentrations and β-glucosidase, respectively. Six strains of probiotic …
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Enzymatic production of oligosaccharides in centrifugal fields
… by the trans-galactosyl activity of the enzyme β-galactosidase on lactose monohydrate was identified as a model enzymatic system to elucidate the principles of this type of process. Galacto-oligosaccharides have attracted considerable commercial interest as food additives which have been shown to …
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Characterizing potential secretion components that increase secretion of recombinant proteins in Pichia Pastoris
… These mutants have the ability to secrete β-galactosidase at higher levels in comparison to the wild type. This study focused on characterizing the specific mutant ah2 for its ability to secrete HRP, SLPI, and CALB lipase proteins, as well as using transmission electron microscopy to observe …
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Probiotic metabolism of human milk oligosaccharides (HMOs) and prebiotics
… L. acidophilus NCFM and further investigated a -galactosidase gene involved in LNnT utilization by L. acidophilus NCFM. β-galactosidase lacL gene knockout in L. acidophilus NCFM and subsequent carbohydrate utilization analysis demonstrated that LNnT was unable to be utilized by the knockout …
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Molecular characterization of ovine GM1 gangliosidosis
… In humans and other species, mutations in the β-galactosidase (GLB1) gene are responsible for similar disease phenotypes. The production of non-functional β-galactosidase results in a buildup of GM1 gangliosides and other terminally linked galactose-containing molecules in the brain and visceral …
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Analyzing the interactions of thermoresponsive coacervate-forming biodegradable polyester encapsulation on model protein structure and activity
… that TR-PEs encapsulated and released active β-galactosidase enzyme. Encapsulation was visualized using confocal fluorescence microscopy and by labeling β-galactosidase with fluorescein isothiocyanate. Interactions between <sup>15</sup>N-isotopically labelled human ubiquitin c and TR-PEs were …
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Evaluation of a Typical CIP Protocol to Control Bacterial Biofilms on Whey Reverse Osmosis Membranes
… especially the enzyme cleaning step. β-galactosidase enzyme was observed to be the most effective under static and dynamic conditions. In addition, a .Protease and a Lipase were also observed to be effective against biofilms developed using the most resistant Bacillus isolate (5.10.3). …
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Isolation of a human milk sialyloligosaccharide by affinity chromatography with wheat germ agglutinin (WGA)
… of the WGA-bound fraction with jack bean β -galactosidase and β -hexosaminidase demonstrated the presence of a lacto-N-neohexaose core. The position of sialic acid in the sialyllacto-N-neohexaose was determined by simultaneous digestion of the sialylhexaose with jack bean β -galactosidase and …
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Enhanced β-galctosidase activity in probiotics for improved bioconversion of soy isoflavones in dairy and soy-based yoghurt
… of whey as a medium for the production of β- galactosidase (β-gal) from Lactobacillus delbrueckii ssp. bulgaricus ATCC 11842 (LB) and Bifidobacterium animalis Bb12 (BB12). The study was undertaken to examine and compare the influence of selected prebiotics, i.e. galactooligosaccharides (GOS), …
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Cationic liposome-mediated transfection of mammary epithelial cells with AAV-based plasmid DNA
… low, with only 2.1% of cells found to express β-galactosidase. To establish the efficacy of chemical-mediated delivery of plasmid DNA in vivo and to investigate an intramammary route of delivery, lactating rats were transfected in vivo with plasmid DNA complexed with LipofectAMINE. These …
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Towards Accessible Diagnostics: Developing CRISPR-Based Colorimetric Tools for Low- and Middle-Income Countries
… immobilising an oligo covalently linked to a β-galactosidase (LacZ) enzyme, which is cleaved in the presence of DNA target-activated CRISPR-Cas12a. This method demonstrated a lower limit of detection than standard fluorescent assays while remaining active for four weeks after lyophilisation at …
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Regulation of Senescence Phenotypes by TIS21 gene in Huh7 Human Liver Cancer Cells
… 실험하였다. 먼저 TIS21을 단독으로 발현시켰을 경우 control에 비해 SA-β-galactosidase 활성도가 미약하게 증가하였고, proliferation이 억제되었으며 , actin filament가 가늘어지고 끊어지는 현상이 관찰되었다. 이와는 반대로 doxorubicin으로 노화를 유도한 간암세포에 TIS21을 과발현 시킨 경우 SA-β-galactosidase 활성도가 떨어졌고, doxorubicin에 의해 유도되어지는 actin filament의 remodeling이 감소하였으며, doxorubicin에 …
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PRINTING MUCOADHESIVE AND ORODISPERSIBLE FILMS FOR THE PERSONALIZATION OF THERAPY
… the preparation of ODF containing the enzyme β-galactosidase, and compared with solvent casting. While HMRE resulted in the thermal degradation of the protein, enzymatic activity was completely preserved in the ODF obtained by solvent casting, which were able to digest lactose contained in milk …
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Translation of Partially Decayed Messenger RNAs in Yeast
… gene, <em>LacZ</em>, encoding the enzyme β-galactosidase. Through the use of primer extension, βgalactosidase assay, and western blots, the results indicate that the partially-decayed RNAs are successfully translated, and that addition of xrRNAs results in a 30-50 fold increase in measured …
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The kinetics of endosome processing
… was removable by subsequent treatment with β-galactosidase.
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