Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"α5β1"”.
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Generierung von Antikörper-Bibliotheken und Selektion von Antikörpern gegen die Integrine αvβ5 und α5β1 mittels Phagen-Display-Technologie
… gegen die Integrine αvβ5 und α5β1 generiert und spezifische Fab-Antikörper mittels Phagen-Display-Technologie selektiert. Dafür wurden zum einen das Antikörper-Repertoire immunisierter Kaninchen und zum anderen die humane Fab-DNA-Vorlage des Plasmids pAra H6HA-TT genutzt, um …
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Correlation between the expression of integrins and their role in cancer progression. Expression pattern of integrins αvβ3, αvβ5 and α5β1 in clinical and experimental tumour samples
… investigate the effect of novel antagonists of α5β1, αvβ3 and αvβ5 integrins on cancer cell migration, a key process in tumour cell dissemination. Immunohistochemistry was used to evaluate the expression of α5, αv, β3 and β5 integrin subunits in prostate cancer tissues. Furthermore the …
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Integrin-independent activation of Tie2 using the de novo designed protein H8mb
… Angiopoietin-1 (Ang1), which binds both Tie2 and α5β1 integrin. Here we report the de novo design of a compact Tie2 binding protein that overcomes these constraints. We use RFdiffusion to design a stable, high-affinity Tie2 binder that functions as a selective antagonist when monomeric, but as a …
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Analyzing Interactions Between Cells And Extracellular Matrix By Atomic Force Microscopy
… integrin α1β1 and fibronectin-binding integrin α5β1 was identified in HeLa cells. This crosstalk was directional from integrin α1β1 to integrin α5β1 and appeared to regulate integrin α5β1 by inducing its endocytosis. In the fourth and final chapter, mechanisms of matrix-induced cell alignment …
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Syndecan-4 regulates cell-surface trafficking and biological activity of transglutaminase-2
… a non-enzymatic process independent from the α5β1 integrin-binding to the RGD domain on FN (Verderio et al., 2003). Hence HS proteoglycan (HSPG) could act as cell surface co-receptor for FNbound TG2 or contribute to the regulation of extracellular TG2 activity in cell adhesion and tissue …
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Influence Of Cholesterol And Bilayer Asymmetry On Membrane Protein Distribution In Polymer-Tethered Raft-Mimicking Lipid Membranes
… and oligomerization state of αvβ3 and α5β1 integrins in raft-mimicking lipid mixtures. In asymmetric bilayers, coexisting lo-ld phase separations are located in the top leaflet, while the bottom leaflet exhibits a homogeneous ld phase. The comparison of symmetric</p> <p>bilayers with …
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Roles For B-Raf Kinase In The Specific Regulation of Α4Β1 Integrin In T Cells
… α4β1 integrin, and not other integrins such as α5β1 or LFA-1, or a variety of membrane proteins. We demonstrate a novel role for B-Raf in the selective regulation of α4β1 integrin-mediated adhesion.</p>
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Spatiotemporal organization, regulation and function of traction during neutrophil chemotaxis
… myosin II contractility and is necessary for α5β1-integrin activation and leading edge adhesion. Localized myosin II activation induced by spatially activated small GTPase Rho and its downstream kinase p160-ROCK, as previously reported, leads to contraction of actin-myosin II complexes at the …
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Investigating spatial distribution and dynamics of membrane proteins in polymer-tethered lipid bilayer systems using single molecule-sensitive imaging techniques
… that ganglioside GM3 induces dimerization of α5β1 integrins in a CHOL-free lipid bilayer, while addition of CHOL suppresses such a dimerization process. Furthermore, GM3 was found to recruit α5β1 integrin into CHOL-enriched domains, illustrating the potential sig-nificance of GM3 as a …
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Role of ICAM-1, a cell adhesion molecule, in morphological changes of BV2 microglial cell
… β1 integrin a subunit of fibronectin receptor α5β1 even in LPS treated group. LPS also increase the trace number of ramified cells. Therefore; ICAM-1 might play an important role for the LPS-induced morphological changes.
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Functional Investigation of Dual αvβ3 and αllbβ3 Integrin Inhibition in Haematological and Solid Tumour Models
… comprising αvβ3, αvβ5, αvβ6, αvβ8, αllbβ3, α5β1, α8β1 and αvβ1 are related to progress and spread of cancer and poor prognosis. Because of the importance of integrin biology in the regulation of cancer dissemination, the integrin receptors are being utilised as targets to regulate cancer …
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Entamoeba histolytica-Induced Caspase-4 Activation Regulates IL-1β Secretion Through Caspase-1
… via the Gal-lectin, surface EhCP-A5 ligates α5β1 integrin to activate caspase-1 in a complex known as the NLRP3 inflammasome. In this study, we investigated the parasite requirements underlying macrophage caspase-4 and -1 activation and the role caspase-4 play in augmenting pro-inflammatory …
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Elucidating The Igfbp2 Signaling Pathway In Glioma Development and Progression
… demonstrated that 1) IGFBP2 activates integrin α5β1, leading to the activation of key pathways important in glioma; 2) IGFBP2 mediates cell migration pathways through ILK; and 3) IGFBP2 activates NF-kB via an integrin α5 interaction. We then sought to determine whether this was a physiologically …
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The Role of Cell Communication and 3D Cell-Matrix Environment in a Stem Cell-Based Tissue Engineering Strategy for Bone Repair
… dissociation between connexin-43 and integrin α5β1 occurred under communication impairment and could be slightly restored with the application of nitric oxide. Thus, in order for osteoblasts to perceive a mechanical stimulus and initiate mineralization of the matrix in response, functional gap …
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Engineered biomaterials as extracellular microenvironments for guiding cell programming and reprogramming
… a mechanism where perimeter features initiate α5β1 adhesion and epithelial-to-mesenchymal transition, Mitogen Activated Protein Kinase (MAPK) and Signal Transducer and Activator of Transcription (STAT) pathways, and regulation of distinct histone marks, to guide gene expression underlying the …
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Modulation of Endothelial Cell Adhesion to Synthetic Vascular Grafts Using Biotinylated Fibronectin in a Dual Ligand Protein System
… SPR results showed statistical differences in α5β1 and αvβ3 integrin binding to RGD cell binding motifs introduced by bFN(9) and RGD-SA. Increase in binding specificity through these integrins lead to rapid cell binding and retention on Teflon-AF surfaces adsorbed with this protein formulation. …
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INTEGRIN ALPHAVBETA3 AS THERAPEUTIC TARGET AND IMAGING BIOMARKER FOR VASCULAR ENDOTHELIAL CELLS AND CANCER EPITHELIAL CELLS
… sequence recognizing integrins αvβ3, αv5 and α5β1, are very important in the regulation of cell growth, survival and migration during angiogenesis and lymphangiogenesis. In particular, the αv integrins have key roles in embryonic development of blood vessels in placenta and brain, whereas …
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Exploring Critical Vehicle Parameters for the Design of Multitargeted Nanoparticles for Cancer Specific Gene Delivery
… to cells with various receptor expression. α5β1 and α6β4 integrin expression levels were then quantified on 20 different cell lines to identify appropriate model cells for in vitro investigation. Dual-targeting heteromultivalent liposomes were synthesized using the alpha6 targeting peptide …