Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 21 for “"α-ketoglutarate"”.
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Genetic Determinants of Cancer Cell Survival in Tumor Microenvironment Stresses
… program. Metabolomic analysis found that while α-ketoglutarate levels decrease under hypoxia in control cells, α-ketoglutarate was paradoxically increased under hypoxia when ACC1 or ACLY were depleted. Supplementation with α-ketoglutarate rescued the hypoxia-induced apoptosis and recapitulated the …
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The oxidative metabolism of malate plus pyruvate, and a-ketoglutarate by vitamin E-deficient rat liver mitochondria
… endogenous, pyruvate plus malate, and α-ketoglutarate, were measured by oxygen electrode analysis. Statistically significant differences were seen among state 4 respiration with α-ketoglutarate, state 4 respiration(combined data with endogenous, pyruvate plus malate and α-ketoglutarate), …
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Structural and functional investigations of mechanisms of iron-utilizing enzymes
… formation by an mononuclear nonheme iron(II) α-ketoglutarate dependent dioxygenase, ScoE. The first part of this thesis focuses on mononuclear nonheme iron(II) α-ketoglutarate dependent dioxygenases: enzymes which use molecular oxygen, α-ketoglutarate and a mononuclear Fe(II) cofactor to …
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A 450K arsenite-binding protein of the rabbit liver cytosol
… acid, such as pyruvate dehydrogenase and α-ketoglutarate dehydrogenase, by presumably binding to these dithiol functional groups. Thin-layer chromatography and mass spectrometry established that arsenite complexes with reduced lipoic acid and might be binding to similar dithiol groups on the …
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Metabolic engineering aimed at the production of keto acids from glycerol : an industrial by-product
… at the enhanced formation of pyruvate and α-ketoglutarate (KG). Under the influence of H2O2, P. fluorescens engineers an intricate metabolic network to synthesize ATP and pyruvate. As oxidative phosphorylation is severely impeded, the microbe invokes substrate level phosphorylation to …
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Mining the enolase superfamily for new functions: investigations of D-glucarate dehydratase related proteins (GlucDRP) and L-lyxonate dehydratase proteins (LyxD)
… act to convert the product to yield α-ketoglutarate semialdehyde. In the final step a dehydrogenase oxidizes α-ketoglutarate semialdehyde to α-ketoglutarate, an intermediate in the citric acid cycle. Mutational studies revealed that L-lyxonate dehydratase proteins possess a catalytic …
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Ketoacids as antioxidants in human astrocytes exposed to oxidative and aluminum stress.
… carnitine homeostasis due to the shunting of α-ketoglutarate (αKG) towards reactive oxygen species (ROS) detoxification. The purpose of this study was to elucidate the role of ketoacids such as oxaloacetate (OAA) and αKG in combating oxidative stress in astrocytes. The present study has shown …
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Quantifying the Intracellular Metabolic Network that Establishes the Simultaneous Utilization of Sugars and Aromatic Substrates in Pseudomonas putida KT2440
… flux through isocitrate dehydrogenase and α-ketoglutarate dehydrogenase. In addition, feeding on the substrate mixture induced carbon overflow from the TCA cycle primarily through a significant involvement of malate dehydrogenase activity over the ED pathway in the biosynthesis of pyruvate. …
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<i>PTHR1/SOX9</i> and <i>IDH1/IDH2</i> Relative Expression in Primary Chondrocyte and Chondrosarcoma Cells Under the Synergistic Influence of Inducible Hypoxia and Extracellular Acidosis
… metabolic enzymes that convert isocitrate to α-ketoglutarate (α-KG). Their mutant counterparts further convert α-KG into a competitive oncometabolite D-2-Hydroxygluterate (D-2-HG). Our colorimetric assay data suggest that D-2-HG concentration levels are 10.5-fold and 6-fold more elevated in …
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Generation of a Medaka Fish Model of Propionic Acidemia for Development of Novel Therapies.
… therapy comprising sodium citrate, ornithine α-ketoglutarate, and glutamine resulted in significant improvements of both survival and locomotor activity in <i>pccb<sup>del/del</sup></i> larvae. <br></br><br></br> Given its small size, easy handling and large number of progeny, the PA medaka …
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Microbial synthesis of antimalarial compound FR-900098: pathway characterization and engineering
… for FR-900098 biosynthesis, the Fe(II) and α-ketoglutarate dependent hydroxylase FrbJ was determined to be involved in the synthesis of another phosphonate antibiotic FR-33289. We demonstrated its ability to catalyze the hydroxylation of additional substrates as part of the creation of a new …
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The role of hypoxia in T cell function and immunotherapy
… metabolite. Glutarate was found to inhibit α-ketoglutarate dependent reactions and to modulate T cell differentiation and improve antitumour CD8+T cell function. Administration of esterified glutarate in tumour-bearing animals significantly improved infiltration of CD8+ T cells in tumours and …
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Metabolic Coordination of Stem Cell Fate Controls Tumor Initiation and Tissue Repair
… which in turn produces the metabolite α-ketoglutarate (αKG). αKG stimulates terminal differentiation via activation of αKG-dependent dioxygenases that remove the repressive histone modification H3K27me3, which otherwise promotes EpdSC self-renewal. Accordingly, serine starvation or …
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Studies of protein complexes involved in the adenylation cascade of the nitrogen signalling pathway in Escherichia coli.
… R domain of ATase. Both PII and ATase bound α-ketoglutarate (α-kg) in direct binding assays. Several lines of evidence suggested that PII contains the high affinity α-kg binding site and ATase the low affinity site. This study demonstrates for the first time that the two small …
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Preclinical Studies of A Novel Idh1 Inhibitor In Acute Myeloid Leukemia (Aml)
… the oxidative decarboxylation of isocitrate to α-ketoglutarate (α-KG) in the citric acid cycle. Somatic gain-of-function mutations in IDH1 occur in ~10% of the newly diagnosed AML patients. The neo-enzymatic activity of mutant IDH1 results in accumulation of the oncometabolite 2-hydroxyglutarate …
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Metabolic pathways of Campylobacter fetus
… acid cycle was actively operating except that α-ketoglutarate dehydrogenase was undetectable in 482 and PB1, and very weak in H840. The interruption in the cycle was supported by the reaction of isocitrate lyase which is a key enzyme of the glyoxylate bypass, and was found in the three strains. …
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The stimulation or inhibition of growth and acid precipitable material production of Vibrio Fetus by Kreb's cycle intermediates, fatty acids, amines, and miscellaneous compounds
… which increased the growth of V. fetus. Alpha-ketoglutarate and tween 80 increased APM production of V. fetus, although these compounds neither increased nor decreased the growth of the vibrios. Therefore, the addition of α-ketoglutarate and tween 80 to the medium used for maximal production of …
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Mitochondrial dysfunction in hereditary optic neuropathies
… treatment based on supplementation of α-ketoglutarate and aspartate.
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Genetic and biochemical analysis of nitrogen metabolism in ruminal anaerobic bacteria: a comparison between ruminococcus albus 8 and prevotella ruminicola 23
… the further transformation from glutamate to α-ketoglutarate by the up-regulated NADH-dependent glutamate dehydrogenase (NADH-GDH) was more evident during late exponential phase. During this phase an ammonium transporter was also up-regulated, apparently as an attempt to enhance ammonia uptake …
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Myoglobin redox form stabilization: role of metabolic intermediates and NIR detection
… (MMb) reduction and oxidation of malate to α-ketoglutarate with regeneration of reduced nicotinamide adenine dinucleotide (NADH) via malate dehydrogenase (MDH). Our specific objectives for this experiment were: (1) to examine the interaction of malate and MDH to reduce MMb in vitro, (2) to …
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