Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 56 for “"α-helix"”.
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Investigating protein folding by the de novo design of an α-helix oligomer
… de novo. The sequence was selected to induce helix formation with a predefined tertiary packing interface. Circular dichroism showed that Ksα1.1 formed α-helical secondary structure as intended. The nuclear magnetic resonance studies demonstrated formation of a high order oligomer with …
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The characterisation of aspects related to the single stranded RNA binding ability of African horsesickness virus nonstructural protein NS2
… affinity for virus specific mRNAs. NS2 has an α-helix rich C-terminal region and α-helixes are known to be involved in ssRNA binding. BTV NS2 has an α-helix content of 69% and AHSV NS2 an α-helix content of 47%. AHSV NS2 has a lower binding affinity for ssRNA than bluetongue virus (BTV) NS2. …
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Structural Analysis of the 50S Ribosomal Stalk
… flexiblen Loop mit einer langen C-terminalen α-Helix verbunden war. Die Helix zeigte in verschieden Kristallformen unterschiedliche Orientierungen in Bezug zur NTD des L10 und beinhaltete die drei aufeinander folgenden Bindungsstellen für die L12 NTD-Dimere. Das Dimerisierungsmuster der L12 NTDs …
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Structural and mutagenesis studies of soluble methane monooxygenase reductase from Methylococcus capsulatus (Bath)
… a six-stranded antiparallel β-barrel and one α-helix, and an NADH binding domain with a five-stranded parallel β-sheet surrounded by four α-helices. The FAD cofactor is bound at the interface between the two domains in a novel conformation. Near this FAD cofactor, a conserved C-terminal …
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EFFECT OF GENOTYPES, TANNIN LEVEL AND PROCESSING METHODS ON THE PHYSICOCHEMICAL, NUTRITIONAL AND STRUCTURAL CHARACTERISTICS OF FABA BEAN GROWN IN WESTERN CANADA
… to total CHO (TCHO) ratio. Amide I area and α-helix absorbance were not different among low tannin genotypes; however, higher ratios for α-helix to β-sheet height and CEC to STCHO were observed. Related to heat-induced changes, Steam Pressure treatment in low tannin genotypes increased (P < …
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Pilus biogenesis in uropathogenic Escherichia coli : An electrophysiology study on the PapC usher
… was applied to investigate the roles of the α-helix, plug, N- and C-terminal domains on the usher channel behavior. Domain deletion mutants characterized using planar lipid bilayer showed the modulating effects of the α-helix, plug, N- and C-terminal domains on the usher channel behavior. In …
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Development of Specific and Potent α-Helical Inhibitors and Probes of Cysteine Proteases
… has an endogenous inhibitor that forms an α-helix at the prime side of the active site. We developed a new method for stabilizing α-helices using natural amino acids that allowed us to make small peptides into α-helical inhibitors. We were then able to use structure based design to turn these …
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An investigation of the interaction between the immune system GTPase GIMAP6 and an autophagy gene 8 homologue GABARAPL2
… Within GABARAPL2, deletion of the N-terminal α-helix resulted in loss of the interaction. A chimeric protein in which the correspond- ing region in MAP1LC3B, a protein unable to interact with GIMAP6, was replaced by GABARAPL2’s N-terminal α-helix reproduced the interaction suggesting that this …
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Identification and activity assessment of a bioactive peptide QUB-2642 from the skin secretion of The American Bullfrog (Rana catesbeiana)
… >512 µM) upon horse erythrocytes. With an α-helix in supposition, the second structure of QUB-2642 was tentatively calculated and discussed. Given the huge potential of endogenous peptides in drug discovery, further verification and modification of QUB-2642 will be necessary for addressing …
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Enzyme Architecture and Flexibility Affect DNA Topoisomerase I Function
… domains) that lies at one end of a short α-helix, which extends to the active site Tyr covalently linked to the DNA. However, in the presence of drug, the linker is rigid and this α-helix extends to include Gly and the preceding Leu, suggesting a dynamic interplay between active site α- …
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Folding, dynamics and interaction studies of the Neuropeptide Y family.
… important secondary structural elements: the α-helix and hairpin-like structure. Therefore, further analysis of the three most prevalent helical secondary structures found in nature (α-, 3₁₀-, π-helix) and the β-hairpin structure were carried out with carefully designed peptide models to …
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Investigation of the Role of Amide I to Amide II Ratio and Alpha Helix to Beta Sheet Ratio of Faba Bean Seeds in the Determination of Microbial Protein Synthesis and Animal Performance and Metabolism in Ruminant Livestock Systems.
… influence of amide I to amide II ratio, and α-helix to β-sheet ratio (α_β) on the protein quality (solubility and digestibility), metabolism, and bioavailability in dairy cows has not been established. Thus, the objective of this research was to evaluate the role of the protein-related spectral …
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Characterization of an Amphipathic Alpha-Helix in the Membrane Targeting and Viral Genome Replication of Brome Mosaic Virus
… examined the possible role of an amphipathic α-helix, helix B, in BMV 1a's membrane association. Deletion or single substitution of multiple amino acids of helix B abolished BMV 1a's localization to nuclear ER membranes. Additional reporter-based, gain-of-function assays showed that helix B is …
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<em>Agrobacterium</em> Virb10 Contributions to Type Iv Substrate Secretion, T-Pilus Biogenesis, and Outer Membrane Pore Formation
… N-terminus, (ii) single transmembrane (TM) α-helix, (iii) proline-rich region (PRR), and (iv) large C-terminal modified β-barrel. I introduced cysteine insertion and substitution mutations throughout the length of VirB10 in order to: (i) test a predicted transmembrane topology, (ii) identify …
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Structure, dynamics, and interactions of Angiotensin family of peptides with functional implications.
… Interestingly, these change from the polyproline helix for AngI to the left-handed helical conformation for AngII, III, and IV. Additionally, the antagonist, Ang₁₋₇, was found to contain a significant population of right handed α-helix. Anisotropy decay measurements with picosecond time resolution …
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NMR Studies of the RNA Binding Domain of U1A protein and its Complexes with RNA
… of the free U1A protein revealed a third α-helix at its C-terminus, which had not been discovered previously. The partial assignment of the complex with the RNA hairpin confirmed and extended a model of the U1A/RNA complex. Comparison of NMR spectra of the two complexes showed that U1A …
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An examination of novel archaeal stress response genes
… the SRC proteins consists of a relatively long α-helix flanked by two small β-strands, followed by a second α-helix, and then three further β-strands. The srd family encodes proteins that are homologous to the C-terminal half of members of the Lrp/AsnC family of transcriptional regulators …
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Characterization of Novel Substrates of the Tankyrase and RNF146 Destruction Complex and Mechanisms of its Regulation
… and shown it to be composed of a novel two α-helix GEF domain. My work has demonstrated that SH3BP5 and SH3BP5L are both required for optimal activation of Rab11a in epithelial cells during lumenogenesis and that their activities are repressed by Tankyrase-mediated PARylation. RNF146 regulates …
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Design and synthesis of molecular tools for the ubiquitin proteasome system
… interaction (PPI) is regulated by the α-helix 9 of E2-25K. Creating a short helical peptide analogous to α-9 of E2-25K with the technique of peptide stapling could potentially block the action of Ubb+1 and provide a tool to investigate the role of the UPS in AD.Following synthesis of the …
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Ultraviolet and Infrared Spectroscopy of Synthetic Foldamers, Aib Homopeptides, and Solvated 1,2-Diphenylethane in the Gas Phase
… and subsequently form the first portion of an α-helix is presented. Additionally, tests of the propensity for (Aib)n homopeptides to form 310-helices, in spite of accumulation of a macrodipole moment are shown. In fact, Aib is a strong 310-helix former, but there appears to be a point around n = …
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