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Showing 1 to 20 of 48 for “"α-amylase"”.
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Human α-amylase: genetic, biochemical and clinical heterogeneity
This document only includes an excerpt of the corresponding thesis or dissertation. To request a digital scan of the full text, please contact the Ruth Lilly Medical Library's Interlibrary Loan Department (rlmlill@iu.edu).
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Proteinaceous inhibitors of α-amylase and α-glucosidase from common bean (Phaseolus vulgaris L.): biochemical characterization and phylogenetic analysis of Sardinian cultivars
… bioactive compounds. Among these proteinaceous α-amylase inhibitors (α-AIs) and α-glucosidase inhibitor could play an important role in weight loss and control of glycemic index above all in overweight and diabetic subjects. In this study 10 poorly studied Italian P. vulgaris cultivars were …
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The cloning and characterization of an α-amylase and a branching enzyme from Butyrivibrio fibrisolvens H17c and their expression in Escherichia coli
… expressing amylolytic activity and encoding an α-amylase was located on a 5.0 kb DNA fragment and expressed from its own promoter in E. coli. It was shown that more than 86% of the amylolytic actvity was located in the periplasm of the E.coli host and TnphoA mutagenesis indicated the presence of a …
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Maize alpha-amylase: purification and properties and induction by gibberellic acid
Alpha-amylase synthesis can be induced in wheat and barley half-seeds by addition of gibberellic acid (GA) to the incubation medium. In maize, induction in de-embryonated kernels by exogenous GA has been reported in some studies but not others. Alpha-amylase induction was investigated in maize by …
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Effect of germination and drying on enzyme activity in sorghum and cowpeas
… 2.5, 5, 7.5 and 10 hours, were evaluated for α-amylase, β-amylase and endo-(1,3)(1,4)-β-D-glucanase activity. Germination had a highly significant effect on enzyme activity (P < 0.01). However, cowpea endo-(1,3)(1,4)-β-D-glucanase was not significantly affected by the combined action of …
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Anti-Diabetic and Anti-Obesity Activities of Cocoa (Theobroma cacao) via Physiological Enzyme Inhibition
… abilities to inhibit α-glucosidase, pancreatic α-amylase, lipase, and dipeptidyl peptidase-IV (DPP4) in vitro were compared between unfermented bean (UB), fermented bean (FB), unfermented liquor (UL), and fermented liquor (FL) cocoa extracts. Overall, there were significant decreases (p<0.05) in …
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Synthesis of Glitazone Analogues as Anti-Diabetic Drugs
… activities against α-glucosidase and α-amylase. Among all the synthesized compounds, 36f exhibited excellent antidiabetic activities against α-glucosidase. Compounds 36a, 36b, 36i and 36j also displayed good activities and have potential to be further investigated whereas compounds 36c, …
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Optimisation of the preservation of microbial cell banks for enhanced fermentation process performance
… licheniformis cell banks, used as inoculum for α-amylase producing 5 L batch fermentations. The effect of the presence of various cryopreservants including glycerol, Tween 80 and dimethyl sulphoxide on final fermentation performance measured by biomass and α-amylase concentration was investigated …
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Biochemical characterisation of an alpha-amylase with pullulan hydrolase type III characteristics derived from a hot spring metagenomics library
… low sequence identity to a characterised α-amylase (62.71%) and 61.66% to a characterised pullulan hydrolase type III, and was, therefore, hypothesized to be an α-amylase (EC 3.2.1.1) with pullulan hydrolase type III (EC 3.2.1.x) characteristics. Further sequence analysis indicated that …
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Antioxidant and antihyperglycemic potential of soybean hydrolysates
… with potent AC as well as α-glucosidase and α-amylase inhibitory activities. Nine protein-based beverages and ten functional ingredients (I-5 to 14) were analyzed for their AC and starch-hydrolyzing enzymes inhibitory activity. Four beverages and four protein-based ingredients were selected for …
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Construction of an efficient secretion system for recombinant proteins in Bacillus subtilis
… proteins in Bacillus subtilis using an a-amylase as a reporter enzyme, which is secreted into the medium using the Sec pathway. Its gene amyQ was fused to an IPTG-inducible promoter. It turned out that increasing amounts of IPTG did not result in a concomitant increase of secreted …
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In vitro and in vivo effects of sorghum condensed tannins encapsulated in kafirin microparticles on digestive amylases
… diabetes (T2D) treatment is to inhibit digestive amylases and thereby decrease post-prandial hyperglycaemia. Polyphenols like sorghum condensed tannins (SCT) inhibit α-amylase and α-glucosidase enzymes. However, orally administered SCT may have poor inhibitory action against amylases, due to …
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The stability of enzymes during breadmaking and their influence on staling characteristics
… methods which are able to specifically measure α-amylase and ß-amylase, in the presence of the other potentially interfering activity, have been evaluated. These have been applied to an investigation of enzyme levels at various stages during the breadmaking process, using a cereal source of the …
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Vergleichende biochemische und strukturelle Untersuchung thermophiler α-Amylasen
… T. maritima. Die rekombinante intrazelluläre α-Amylase aus T. maritima, die ihre maximale Aktivität bei 90 °C erreicht, besitzt eine ungewöhnlich hohe Empfindlichkeit gegenüber chelatierenden Agenzien und ihre spezifische Aktivität wird durch Reduktionsmittel gesteigert. In T. maritima ist das …
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The Utilization of Dietary Carbohydrates by Trout and Carp
… liver damage, or persistent hyperglycaemia. α-amylase and β-glucosidase activities increase initially with increase in dietary dextrin, and is higher in Carp than in Trout. 45% dextrin in the diets of carp produced the highest levels of α-amylase in the liver and β-glucosidase in the hind-gut. …
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Isolation and characterization of rice (Oryza sativa L.) β-Glucosidases
… and enzymatic activity of β-glucosidase and α-amylase were studied. To study histochemical localization of rice β-glucosidase, the chromogenic substrates were used. The substrates were incubated with cross and longitudinal sections of whole seeds and seedlings, tissue sections, protoplast and …
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Characterization of blackcurrant berries (Ribes nigrum) and the evaluation of their bioactive compounds after ultrasound-assisted water extractions, enzymatic treatments, and fermentation
… of BC and their effect on the activities of α-amylase, α-glucosidase, DPPIV, as biochemical markers of diabetes. In addition, the antioxidant capacity was measured using 2,2- diphenyl-1-picrylhydrazyl (DPPH·), after water-based ultrasound assisted extraction, treatment with pectinase and …
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Studies on quinoa (Chenopodium quinoa) for novel food and beverage applications
… showed radicle protrusion after 8-9 h. The α-amylase activity was very low, and started to increase only after 24 hours of germination in the starchy perisperm. Proteolytic activity was very high in dry ungerminated seeds, and increased slightly over 24 h. A significant fraction of this …
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Evaluation of six plant species used traditionally in the treatment and control of diabetes mellitus
… test the efficacy of different extracts on alpha amylase (carbohydrate digestive enzyme) activity, alpha glucosidase (glucose absorption) activity and islets of Langerhans insulin secretory activity. Hexane, ethyl acetate, acetone and methanol extracts were examined and screened for their …
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A NOVEL MAGNETIC THREE-DIMENSIONAL LEVITATED PRIMARY CELL CULTURE SYSTEM FOR THE DEVELOPMENT OF IN VITRO SALIVARY GLAND-LIKE ORGANOIDS
… and neuronal). Intracellular calcium and α-amylase activity assays showed functional organoids with SG-specific secretory activity. Thus, these outcomes suggest that M3DL is a promising tool to generate SG-like organoids for SG repair.
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