Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"α-Hemolysin"”.
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Analysis of Polyethylene Glycol in the α-Hemolysin Nanopore
… molecules of interest. These pores are usually α-hemolysin which is a toxin from Staphylococcus aureus or more recently nanoscale synthetic solid state pores. Now we are beginning to look at other molecules or proteins by sending them into the nanopores and measuring a characteristic partial …
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Selective molecular transport in nanopore systems
… probe capture in bacterial toxin protein α-hemolysin (aHL) using dielectrophoresis. Third, I introduce the first simulation of molecular artificial water channel pillar[5]arene (PAP) and uncover the mechanics of its water transport and self-aggregation properties. Fourth, I characterize the …
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Intercellular communication in uropathogenic Escherichia coli and the probiotic Lactobacillus reuter
… motility or biofilm formation, but did alter α-hemolysin activity. L. reuteri RC-14 was shown to produce AI-2 in non-traditional expression patterns. Complementation of the E. coli J96 IuxS mutant with L. reuteri RC-14 luxS showed that the anaerobic, gram positive, luxS gene is functional in the …
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Isolation and Use of the Aeromonas hydrophila Secretin ExeD for Nanopore Analysis
… the size of the most commonly used nanopore, α-hemolysin. The narrow vestibule of the pore doesn’t allow proteins or peptide aggregates to pass through its channel. A larger pore, using the type II secretin of Aeromonas hydrophila, may be able to circumvent the size constraint of α-hemolysin and …
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Nanoscale control in biological and synthetic systems
… translocation through the biological nanopore α-hemolysin. I have also employed MD simulations to explore methods for enhancing experimental control over the translocation of DNA through synthetic nanopores.
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Staphylococcus aureus virulence factors dictate host signaling pathways and immune responses
… genes in MAPK p38 phosphorylation and α-hemolysin in ERK phosphorylation and IL-8 expression in PMN. Continuing our studies of VF, chemotaxis inhibitory protein of S. aureus (CHIPS) inhibits monocyte chemotaxis. We hypothesized that CHIPS inhibited C5a receptor (C5aR) signaling. Monocytes …
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Nanoscale transport simulation of ion channels
… diameter. In particular we have studied the α-hemolysin channel with covalently attached molecular adapter β-cyclodextrin. Although the wild type of this ion channel is a toxic protein in nature, its engineered mutations with molecular adapters could be key components of fabricated nanoscale …
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Nanopore-based Protein Sensing with DNA Nanostructures
… ago, scientists created the first nanopore- an α-hemolysin channel inserted into a lipid bilayer separating two buffered KCl-filled compartments. Today, nanopores are often associated with sequencing – likely due to the immense success of companies like Oxford Nanopore Technologies. However, …
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Repurposing non-antimicrobial drugs to treat multi-drug resistant bacterial and fungal infections
… Panton-Valentine leucocidin (PVL) and α-hemolysin (Hla), thereby improving the treatment outcome against toxin-producing bacterial pathogens. Furthermore, all these drugs effectively reduced both the bacterial load and inflammatory cytokines in a mouse model of <em>S. aureus</em> skin …
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Estudo teórico conformacional da delta-toxina e teórico-experimental da interação de complexos de cobre-base de Schiff com o canal iônico da alfa-toxina de Staphylococcus aureus/
O Staphylococcus (S.) aureus é responsável por inúmeros casos de infecções em animais e seres humanos. Essa bactéria secreta uma grande variedade de exoproteínas, incluindo quatro hemolisinas: alfa, beta, gama e delta. A atividade hemolítica da alfa-toxina (α-HL) se deve a formação de poros em …
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Studies of Three Human Intestinal Opportunistic Pathogens
Opportunistic bacterial pathogens are present in the intestines of all mammals. These bacteria are symbionts to a certain extent, but under certain conditions these organisms can be deadly. Intestinal opportunistic pathogens encompass many genera and include organisms such as those in the …