Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"α -tubulin"”.
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Suppression of cold-sensitive extra-microtubule mutants of yeast α-tubulin by overespression of wild-type genes
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1994.
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Evaluation of inhibitors of histone deacetylases as potential neurotrophic agents
… namely, acetylation of histones (H3/H4) and α-tubulin, role of cAMP, and cell- cycle analysis with flowcytometry were also studied. </p> <p>Vorinostat, a pan HDAC inhibitor, independently induced significant neurite outgrowth in NS-1 cells, similar to that of NGF. The inhibitors of MEK1/2 & …
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Modulation of Histone Deacetylases Attenuates the Pathogenesis of Alzheimer's disease
… HDAC6 mit zytoplasmatischen Substraten wie α-Tubulin, einem Bestandteil von Mikrotubuli, und Hsp90, einem molekularen Chaperon, reagiert. Durch seine Wirkung auf α-Tubulin, hat sich gezeigt, dass HDAC6 an der negativen Regulierung des mikrotubulären Transports essentieller zellulärer …
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The role of sphingolipids in autosomal dominant polycystic kidney disease (ADPKD)
… (evident by decreased levels of acetylated α-tubulin) is also a crucial factor in defective renal cystic epithelia. Sphingolipids contribute to regulating these cellular processes. Two isoforms of sphingosine kinase (SK1 and SK2) catalyse the formation of the bioactive lipid …
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Non-Enzymatic Activity Of The Alpha Tubulin Acetyltransferase Alpha-Tat1 Differentially Alters The Stability Of Dynamic And Stable Microtubules
… remains a mystery. We hypothesized that α-tubulin acetyltransferase (αTAT), which both stabilizes long lived microtubules against mechanical stress via acetylation and has been implicated in promoting microtubule dynamics, could play a role in this process. Biophysical reconstitution …
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ANALYZING THE FUNCTIONS OF PREFOLDIN COMPLEX IN NEUROBLAST HOMEOSTASIS OF DROSOPHILA MELANOGASTER
Prefoldin is a molecular chaperone that regulates tubulin function in mitosis. In this thesis, I show that Prefoldin depletion results in disruption of neuroblast polarity, leading to neuroblast overgrowth in Drosophila larval brains. Co-depletion of Prefoldin and Partner of Inscuteable (Pins) …
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Úloha SIRT1 během zrání oocytů v podmínkách in vitro
… specific SIRT1 action is supported decreasing α-tubulin acetylation. Based on the observation of histone code in immature and matured oocytes, we suggest that SIRT1 is mostly predestined for epigenetic mode of action in germinal vesicle (GV) of immature oocyte. Accordingly, SIRT1- driven …
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COMPUTER-AIDED MOLECULAR DESIGN AND MODELING OF TUBULIN TARGETING AGENTS
… neurodegenerative diseases and neurotoxicity. Tubulin protein is the building block of MTs and is the target protein on which the work described in this Ph.D. thesis is focused. Within the TubInTrain program, the objective of my project is to apply a series of computer-aided molecular design …
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3D localization of cytoskeleton in mouse spermatids using stochastic optical reconstruction microscopy
… Our experiments confirmed the presence of α-tubulin in the manchette and that of F-actin in the manchette and the acroplaxome, as previously observed by researchers with 2D confocal images. We observed that the manchette reduces in diameter and progresses to the caudal portion of the cell at …
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Anti-candida and Anti-virulence Activity of Essential Oils and Their Components
… to microtubule stability. Interestingly, both tubulin and Kar3 protein was delocalized with essential oil components exposure at levels for which endogenous ROS levels were normal. I further demonstrate that the level of Kar3 is associated with resistance and susceptibility of C. albicans to …
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Selective histone deacetlyase inhibition decreases disease in lupus-prone mice
Histone deacetylase 6 (HDAC6) is a cytoplasmic enzyme that acetylates several proteins that are involved in the immune response. HDAC6 inhibition has been shown in various models to decrease inflammation by altering various proteins involved in the dysregulation of B and T cell responses. In our …
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Modulation of macrophage function and immune response by a helminth-derived cysteine protease
… which appeared to correlate with altered α-tubulin localisation. Thus suppressed uptake of LPS correlates with the suppression of TRIF-dependent late endosomal signalling. Nanotubes are cellular protrusions which connect cells and are utilised for the transport of cellular components between …
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Development of approaches for investigating the distribution of toxoplasma gondii infection in natural populations of animals and humans
… showed successful amplification of the human α-tubulin gene and were used for T. gondii DNA detection. We used commonly used PCR markers (B1, SAG1, SAG2, SAG3, GRA6, APICO, L358, PK1, SAG1-Su, BTUB, alt.SAG2, c22-8 and c29-2), histological and immunohistochemical staining to confirm the presence …
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Gen- und Proteinexpressionsanalysen an primären Rattenhepatozyten
… Expression von Strukturgenen (z.B. β-Actin, α-Tubulin, Vimentin). Der In vivo-Effekt eines Enzymwechsels während der Leberperfusion von Collagenase D zu Liberase Blendzyme führte zu signifikanten Veränderungen sowohl in der Gen- als auch der Proteinexpression und konnte durch alle angewandten …