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Showing 1 to 12 of 12 for “"[Fe-S] cluster assembly"”.
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Characterization of Fe-S Cluster Assembly On the Sufbc2D Complex
<p>In vivo biogenesis of iron-sulfur (Fe-S) cluster cofactors requires complex biosynthetic machinery to limit release of iron and sulfide, to protect the Fe-S cluster from oxidation, and to target the Fe-S cluster to the correct apo-enzyme. The SufABCDSE pathway for Fe-S cluster assembly in E. …
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Probing Plant Metabolism: The Machineries of [Fe-S] Cluster Assembly and Flavonoid Biosynthesis
The organization of metabolism is an essential feature of cellular biochemistry. Metabolism does not occur as a linear assembly of freely diffusing enzymes, but as a complex web in which multiple interactions are possible. Because of the crowded environment of the cell, there must be structured and …
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Characterizing the Roles of Sufb and Sufa in Fe-S Cluster Assembly and Trafficking in Suf Pathway
<p>Iron-sulfur (Fe-S) clusters are essential cofactors in various metalloproteins and are best known for their roles in electron transport as well as redox and non-redox catalysis. Three Fe-S cluster assembly systems: Nif, Isc, and Suf have been identified. The sufABCDSE operon is activated in E. …
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Biogenesis of iron-sulfur clusters and intracellular iron metabolism
Iron-sulfur ([Fe-S]) clusters represent one of natures most diverse and ubiquitous protein prosthetic groups. [Fe-S] proteins are integral for diverse biological processes. Reconstitution of apo-proteins can occur by exogenously adding excess iron and sulfide. However, due to the toxicity of iron …
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Genes from Arabidopsis involved in iron-sulfur cluster biogenesis
Iron sulfur [Fe-S] proteins are essential components of many major biological processes including electron transport, respiration, photosynthesis, hormone biosynthesis, and environmental sensing. The process of [Fe-S] cluster assembly in living cells is a controlled mechanism that is highly …
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Probing Iron Accumulation in Sacchromyces cerevisiae Using Integrative Biophysical and Biochemical Techniques
Iron is an essential element for life. It is involved in a number of biological processes, including iron sulfur (Fe/S) cluster assembly and heme biosynthesis. However it is also potentially toxic due to its ability to induce formation of reactive oxygen species (ROS) via Fenton chemistry. …
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Biosynthesis of Iron-Sulfur Clusters
It is not known whether biosynthesis of [Fe-S] clusters occurs through a spontaneous self-assembly process or an enzymatic process. However, in the <I>Azotobacter</I> <I>vinelandii</I> nitrogenase system, it has been proposed that NifS and NifU are involved in the mobilization of sulfur and iron …
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Biophysical Investigations of the Cytosolic Iron-Sulfur Cluster Assembly Pathway Late Acting Proteins
The cytosolic iron-sulfur ([Fe-S]) cluster assembly (CIA) pathway matures cytosolic and nuclear iron-sulfur dependent proteins found in eukaryotic cells. [Fe-S] clusters are involved in life-sustaining processes such as: DNA replication and repair, transcription, translation, and nucleotide and …
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Application of hydrogen deuterium exchange mass spectrometry in protein-ligand and protein-protein interactions
… have a great variety of internal motions with different amplitudes and different timescales. These internal motions play an important role in catalytic processes. Therefore, the existence of an intimate relationship between protein dynamics and protein function is widely accepted. Due to the …
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The Biosynthesis and Function of Nitrogenase Metalloclusters
… of N2 to ammonia (nitrogen fixation). The metalloclusters associated with the nitrogenase components include the [4Fe-4S] cluster of the Fe protein, and the P-cluster [8Fe7S] and FeMo-cofactor [7Fe-9S-Mo-X-homocitrate], both contained within the MoFe protein. These metal-complexes play a vital …
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Assembly of Iron-Sulfur Clusters In Vivo
Iron-sulfur [Fe-S] clusters are protein cofactors that facilitate various life-sustaining biological processes. Their in vivo assembly is accomplished by three different systems known to date. These are: the NIF system which provides [Fe-S] clusters for nitrogenase and other nitrogen-fixing …
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The Role Of Cardiolipin In Iron Homeostasis And Glutathione Metabolism
… levels result from perturbations in iron-sulfur (Fe-S) cluster biogenesis. Consistent with an Fe-S defect, deletion of ISU1, one of two ISU genes that encode the mitochondrial Fe-S scaffolding protein essential for the synthesis of Fe-S clusters, led to synthetic growth defects with the crd1Δ …