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Showing 1 to 20 of 38 for “"[Fe-S] cluster"”.
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Characterization of Fe-S Cluster Assembly On the Sufbc2D Complex
<p>In vivo biogenesis of iron-sulfur (Fe-S) cluster cofactors requires complex biosynthetic machinery to limit release of iron and sulfide, to protect the Fe-S cluster from oxidation, and to target the Fe-S cluster to the correct apo-enzyme. The SufABCDSE pathway for Fe-S cluster assembly in E. …
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FUNCTIONAL STUDIES OF THE FE-S CLUSTER BIOGENESIS BY SUF SYSTEM
Iron-Sulfur clusters are important metal cofactors for many enzymes. Bacteria have dedicated systems for the maturation and trafficking of Fe-S clusters. The SUF (Sulfur Utilization Factor) system is the only housekeeping system in Gram-positive bacteria; whereas in Gram-negative bacteria, the SUF …
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Probing Plant Metabolism: The Machineries of [Fe-S] Cluster Assembly and Flavonoid Biosynthesis
The organization of metabolism is an essential feature of cellular biochemistry. Metabolism does not occur as a linear assembly of freely diffusing enzymes, but as a complex web in which multiple interactions are possible. Because of the crowded environment of the cell, there must be structured and …
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Characterizing the Roles of Sufb and Sufa in Fe-S Cluster Assembly and Trafficking in Suf Pathway
<p>Iron-sulfur (Fe-S) clusters are essential cofactors in various metalloproteins and are best known for their roles in electron transport as well as redox and non-redox catalysis. Three Fe-S cluster assembly systems: Nif, Isc, and Suf have been identified. The sufABCDSE operon is activated in E. …
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Novel Electron Transfer Systems in Hyperthermophilic Methanogenic and Anaerobic Methanotrophic Archaea: F420-dependent Nitrite Reductase and [Fe-S] Cluster Assembling Thioredoxin
… redox enzymes and emphasize the electron transfer functions. From an investigation employing Methanocaldococcus jannaschii, an ancient lineage hyperthermophilic methanogen and inhabitant of deep-sea hydrothermal vents, as a model organism, our laboratory has discovered three novel enzymes …
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Characterization of the Role of Glutaredoxin 3 and Its Binding Partners In Maintaining Iron Homeostasis In Saccharomyces Cerevisiae
… of Aft1 is dependent upon mitochondrial Fe-S cluster biosynthesis via a signaling pathway that includes the cytosolic monothiol glutaredoxins, Grx3 and Grx4. The crystal structure of the thioredoxin-like domain of Grx3 has been determined at 1.5 ├â┬à resolution and represents the first …
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Genes from Arabidopsis involved in iron-sulfur cluster biogenesis
Iron sulfur [Fe-S] proteins are essential components of many major biological processes including electron transport, respiration, photosynthesis, hormone biosynthesis, and environmental sensing. The process of [Fe-S] cluster assembly in living cells is a controlled mechanism that is highly …
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Biogenesis of iron-sulfur clusters and intracellular iron metabolism
Iron-sulfur ([Fe-S]) clusters represent one of natures most diverse and ubiquitous protein prosthetic groups. [Fe-S] proteins are integral for diverse biological processes. Reconstitution of apo-proteins can occur by exogenously adding excess iron and sulfide. However, due to the toxicity of iron …
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Probing Iron Accumulation in Sacchromyces cerevisiae Using Integrative Biophysical and Biochemical Techniques
Iron is an essential element for life. It is involved in a number of biological processes, including iron sulfur (Fe/S) cluster assembly and heme biosynthesis. However it is also potentially toxic due to its ability to induce formation of reactive oxygen species (ROS) via Fenton chemistry. …
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Coordination Chemistry of Fe–S Clusters Supported by N-Heterocyclic Carbenes
Iron–sulfur clusters are ubiquitous in Nature and carry out some of the most challenging multielectron redox reactions in the biosphere while utilizing three primary building blocks: Fe²⁺, Fe³⁺, and S²⁻. Studying synthetic Fe–S clusters aids in understanding the underlying properties of Fe–S …
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The Biosynthesis and Function of Nitrogenase Metalloclusters
… of N2 to ammonia (nitrogen fixation). The metalloclusters associated with the nitrogenase components include the [4Fe-4S] cluster of the Fe protein, and the P-cluster [8Fe7S] and FeMo-cofactor [7Fe-9S-Mo-X-homocitrate], both contained within the MoFe protein. These metal-complexes play a vital …
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Synthesis of Isotopically Labeled Fe and S-alkylated Iron-Sulfur Clusters
… (SAM) enzymes (RS enzymes) use a 3:1 site-differentiated [Fe₄S₄]⁺ cluster to reductively cleave the SAM cofactor and generate a 5’-deoxyadenosyl radical intermediate (5’-dAdo•) that regio- and stereospecifically abstracts an H-atom from the target substrate. It has been proposed that 5’-dAdo• …
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Biosynthesis of Iron-Sulfur Clusters
It is not known whether biosynthesis of [Fe-S] clusters occurs through a spontaneous self-assembly process or an enzymatic process. However, in the <I>Azotobacter</I> <I>vinelandii</I> nitrogenase system, it has been proposed that NifS and NifU are involved in the mobilization of sulfur and iron …
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Experimental Characterization of Iron-Sulfur Cluster Excited States and their Relevance to Electron Transfer Reactions
Iron-sulfur (Fe−S) clusters, and cuboidal [Fe₄S₄] clusters in particular, are biologically ubiquitous metallocofactors involved in a diverse array of cellular processes. The electronic structures of these metallocofactors are highly multiconfigurational, and characterized by dense manifolds of …
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Cysteine Desulfurases Mediating Sulfur Trafficking for the Biosynthesis of Fe-S Clusters
Bacteria have developed systems to safely traffic intracellular iron and sulfur for the biosynthesis of Fe-S clusters. These dedicated pathways guarantee the recruitment of individual components, their assembly into clusters and delivery to final targets. Despite variations on pathway composition, …
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Investigating Iron Transport and Utilization Features of Acinetobacter baumannii
… human pathogen that causes severe nosocomial infections. The bacterium must overcome iron starvation and oxidative stress conditions imposed by the host in order to propagate and cause disease. This work further investigates the transport and utilization of iron by <i>A. baumannii</i>, and their …
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Assembly of Iron-Sulfur Clusters In Vivo
Iron-sulfur [Fe-S] clusters are protein cofactors that facilitate various life-sustaining biological processes. Their in vivo assembly is accomplished by three different systems known to date. These are: the NIF system which provides [Fe-S] clusters for nitrogenase and other nitrogen-fixing …
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The Role Of Cardiolipin In Iron Homeostasis And Glutathione Metabolism
… levels result from perturbations in iron-sulfur (Fe-S) cluster biogenesis. Consistent with an Fe-S defect, deletion of ISU1, one of two ISU genes that encode the mitochondrial Fe-S scaffolding protein essential for the synthesis of Fe-S clusters, led to synthetic growth defects with the crd1Δ …
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Structural and functional studies of heme binding proteins toward the understanding of malaria
Malaria, a potentially lethal parasitic infection, causes approximately half a million deaths each year. Currently, nearly half of the world's population is at risk of being infected with Plasmodium, the parasite causing malaria. In this thesis, we use biochemical and biophysical techniques to …
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Biophysical Investigations of the Cytosolic Iron-Sulfur Cluster Assembly Pathway Late Acting Proteins
The cytosolic iron-sulfur ([Fe-S]) cluster assembly (CIA) pathway matures cytosolic and nuclear iron-sulfur dependent proteins found in eukaryotic cells. [Fe-S] clusters are involved in life-sustaining processes such as: DNA replication and repair, transcription, translation, and nucleotide and …
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