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Showing 1 to 20 of 20 for “"; Crystallization studies"”.
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Crystallization studies of Pictet-Spenglerases
… features that impart binding selectivity, crystallization studies of this enzyme were performed. The native enzyme and several interesting mutants were studied; co-crystallization experiments with inhibitors and substrates were also performed. Diffraction quality crystals of the native …
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Crystallization studies of 5'-deoxyadenosyl radical enzymes
… requirements of enzymes in these classes, crystallization studies were undertaken on four enzymes. The structure of the resting form of lysine 5,6-aminomutase, an adenosylcobalamin-dependent enzyme, is known from previous work in our laboratory; however, the structure of a catalytic state …
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Melting and crystallization studies in a partially excluding copolymer
The crystallization and melting behavior of copolymers (specially of those which partially exclude the and component) has not been well understood. Poly(ether ketone ketone) PEKK is such a partially excluding copolymer in which the crystalline phase tends to exclude one of two similar monads. …
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Crystallization studies of liquid crystalline polycarbonates based on substituted stilbene mesogen
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Materials Science and Engineering, 1995.
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Inhibition of Calcium Oxalate Monohydrate Crystallization Using Organic Growth Modifiers
… derivatives of citrate, a known inhibitor of COM crystallization. Bulk crystallization studies revealed the specific binding of modifiers to COM crystal surfaces. Kinetic studies were performed to quantify both the efficacy and potency of growth inhibitors. These studies demonstrated that …
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Structural studies of organic crystals of pharmaceutical relevance. Correlation of crystal structure analysis with recognised non-bonded structural motifs in the organic solid state
… them to the Cambridge Structural Database. Crystallization studies are done on these selected systems as well as exploiting those using synthetic analogues. A total of 51 crystal structures were investigated including 16 new structure determinations. Addition synthesis of new xanthines to …
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Crystallization and Melting Studies of Poly(ε-caprolactone) and Poly(ethylene oxide) using Flash™ Differential Scanning Calorimetry and Preparation and Characterization of Poly(δ-valerolactone) Fractions
The isothermal crystallization and melting temperatures of poly(ε-caprolactone) were correlated using fast differential scanning calorimetry. The melting kinetics was found to be independent of isothermal crystallization temperature and time. The conventional Hoffman-Weeks method could not be used …
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Entwicklung von Fermentationsprozessen zur Produktion rekombinanter Antikörperfragmente in Pichia pastoris und Nicotiana tabacum
… The product purity was sufficient for subsequent crystallization studies.
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Study of the miscibility, morphology, crystallization, and melting behavior of blends of poly(3-hydroxybutyrate) and poly(vinylidene fluoride)
… results of thermal, optical, and spectroscopic studies on poly(vinylidene fluoride)/poly(3-hydroxybutyrate) (PVDF/PHB) blends prepared by melt and solution blending techniques. The blends exhibit - a single, composition dependent Tg. Dynamic crystallization studies indicate that PVDF …
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A study of miscibility, morphology, crystallization and melting behavior of isotactic poly(propylene) in blends of poly(propylene) and poly(1-butene)
… dependent glass transition temperature. Crystallization studies of the a phase of it-PP in these blends show that the spherulitic growth rate of it-PP decreases with increasing it-PBl content. The melting behavior of the it-PP also depends on blend composition. However, polarizing optical …
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The Structural and Biochemical Characterization of Salmonella Host Specificity Determinant Gifsy-2 Gene E
… minimal constructs of these effector domains for crystallization studies, and obtained needle-like spherulites with the C-terminal domain. We also showed that mouse Exo70 is able to pulldown the C-terminal domain of SipC from cell lysate. Finally, we examined Salmonella effector AvrA, which has …
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Nanoscale Confinement Effects on Poly(ε-Caprolactone) Crystallization at the Air/Water Interface & Surfactant Interactions with Phospholipid Bilayers
… effects on poly(ε-caprolactone) (PCL) crystallization were probed through crystallization studies of PCL-b-poly(tert-butyl acrylate) (PCL-b-PtBA) copolymers, PCL with bulky tri-tert-butyl ester endgroups (PCL triesters), PCL with triacid end groups (PCL triacids), and magnetic …
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Towards the Characterization of Enzymes Involved in the Metabolism of Tyrosine and Tyrosine Derivatives
… using various chromatographic techniques, crystallization under different conditions, and its kinetic analysis, and molecular dynamics simulations. Based on sequence, structure, and kinetic data we have shown that mouse TAT behaves like human TAT. Our crystallization studies added new …
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Cloning, Expression and Purification of the Different Human Haptoglobin Chains and Initial Characterization by Mass Spectrometry
… in the protein and degradation during the crystallization process. We were able to identify the cleavage sites in the degradation of the protein by sequence analysis and ESI MS of fresh and degraded protein samples. Using analysis of the charge state distributions in ESI MS, we provide …
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Using molecular dynamics to probe protein conformational transition mechanisms at the membrane surface
… protein, which has remained elusive to multiple crystallization studies, the simulations describe a large, interdomain conformational change that reconciles previous discrepancies between biochemical and structural studies. In addition to the studies on peripheral proteins, MD simulations were …
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Cis and Trans, p53 and NF-kB rules of transactivation
… to function as antagonists, although recent studies identified example of positive, even cooperative interactions. p53 and NF-kB act as dimer or dimer of dimers, bind cis regulatory elements (referred herein as Response Elements REs) of which multiple versions exist in the genome, and …
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Natural and Synthetic Ligand-Binding Induce Different Pilin Assemblies in vitro and Control P. aeruginosa Bioactivities in vivo, and Development of Bacterial-Motility Enabled Binding Assays
… </p> <p>For biochemical and structural studies on pilin protein, we used a common technique of recombinantly expressing truncated pilin in E. coli. The truncation of pilin from the N’-terminal α-helix retains the perceived binding region within the disulfide loop and yields a soluble …
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Structural and ligand binding studies of toll-like receptor 4 (TLR4) associated proteins
… CD14 as an additional target for ligand binding crystallization studies. After determining that purified human and mouse CD14 bind LPS and other ligands using native PAGE gel shift and ForteBio Octet assays, we confirmed the bioactivity of both purified proteins through cell-based stimulation …
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Rarity of kidney stones in South Africa's black population : studies of urinary macromolecules, crystal matrix extract containing osteopontin, and bone turnover markers in urine and serum from black and white subjects as a key to understanding this paradox
… recruited for this purpose. In several of the studies, subjects followed a standardized diet and were required to provide 24 hour urine collections. These were analyzed for sodium, potassium, calcium, oxalate, uric acid, citrate, chloride, magnesium, phosphate, sulphate and creatinine using …
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Rheological and Thermal Insights into Solidification Dynamics in Extrusion-Based Additive Manufacturing of Polymer Composites
… rheological behavior, and heat retention affect crystallization kinetics and polymer chain mobility, relating it to the development of printed microstructure and interlayer adhesion. Strategies to minimize common issues in printing semicrystalline polymers are also included, for example through …