Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 9 of 9 for “"(SEC-MALS)"”.
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Characterization of CAXCK31, a Bacterial Calcium/Proton Antiporter
… used include chemical cross-linking, FRET, and SEC-MALS. In addition, various transport properties of CAXCK31, including substrate selectivity, pH dependence, and transport rates, have been characterized for the first time.</p>
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THE SYNTHESIS AND CHARACTERIZATION OF DUAL-MODIFIED CARBOXYMETHYLCELLULOSE FOR BIOADHESIVE APPLICATIONS
… with multi-angle light scattering (SEC-MALS). The adhesive strength of the material was determined by lap shear testing with porcine skin. High molecular weight and medium molecular weight methacrylated dialdehyde carboxymethylcellulose (MeDCMCHV & MeDCMCMV) were engineered and …
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A screen for novel interactions within the vertebrate centriole
… protein complexes recombinantly expressed in insect cells with the aim of subjecting the complexes to structural/biophysical studies. Only 1 novel interaction eluted (PLK1-KIF24) robustly as a binary complex. I validate this interaction in-vitro using SEC-MALS and map the binding region further …
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INVESTIGATION OF HETEROTRIMER-FORMING COLLAGEN-MIMETIC PEPTIDES THAT INCORPORATE AZA-GLYCINE RESIDUES
… We use circular dichroism, ESI-MS and SEC-MALS studies to develop CMP strands that possess a markedly reduced homotrimer stability, and to gain insight into the molecular species formed in these mixtures. We also present the application of fluorophore-tagged CMPs incorporating the …
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Regulation of inflammatory TLR signalling by the BCAP adaptor
… of BCAP TIR domain interactions. The second aim is to characterise and further explore the BCAP interactome, in the context of TLR and BCR signalling. Identification of novel interaction partners and a more detailed understanding of existing interactions, including PI3K and PLC-𝛾2, is …
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Stability Study of Engineered Ferritin Nano-vaccines by Combined Molecular simulation and Experiments
… Q column to remove the remaining HCPs. The final SEC flow through samples of C1 and C2 had purity levels of over 90% and were suitable for characterisation tests. Unexpectedly, C3 and C4 were unable to be purified to an extremely high purity throughout this process. Following purification, the …
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Insights into the structure and aggregation of lens crystallins and other aggregation-prone proteins
… various conformational aspects in comparison to SEC-MALS. The method distinguished between BSA oligomers and two different monomer populations, multiple YADH monomer and tetramer conformations, and apo- and holo-BLA. The 'dispersity of electrophoretic mobilities' allowed a relative comparison of …
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Insights into the structure and aggregation of lens crystallins and other aggregation-prone proteins
… various conformational aspects in comparison to SEC-MALS. The method distinguished between BSA oligomers and two different monomer populations, multiple YADH monomer and tetramer conformations, and apo- and holo-BLA. The 'dispersity of electrophoretic mobilities' allowed a relative comparison of …
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Characterising the effects of ACK-mediated phosphorylation on the Class IA PI3-Kinase regulatory isoforms
… assays with recombinant ACK. Interestingly, the SEC-MALS experiments with phosphorylated p85α and p55α showed no evidence for phosphorylation-driven p85 isoform dimerisation. Yet, further analysis suggested that the absence of detected dimerisation was likely attributed to low levels of …