Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 74 for “""shotgun" cloning"”.
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Molecular cloning, structural characterisation and functional study of bioactive peptides from amphibian skin secretion
… by use of electrical stimulation. Afterwards, ‘shotgun’ cloning was applied using 3’RACE PCR to obtain full length nucleotide sequence that encoded the open reading frames of peptide biosynthetic precursors. Three putative peptides were identified by cloning (Kunitzin-AH, QUB-1617, QUB-1629), …
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Isolation, characterisation and molecular cloning of a bioactive peptide, QUB 1675, from the defensive skin secretion of Odorrana livida
… precursor of QUB 1675 was identified by using “shotgun” cloning of a cDNA library generated from the polyadenylated mRNA present in the skin secretion of Odorrana livida. To evaluate the bioactivity of this peptide, QUB 1675 was synthesised by solid phase peptide synthesis (SPPS). The synthetic …
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Approach to the identification of sex-determining genes in the tilapia genome by genetic mapping and comparative positional cloning
… in the sex-determining regions were isolated. Shotgun and end-sequences from these BACs identified syntenic regions among Fugu, tilapia, and human. A few genes such as a DEAD box protein, Sox family, and a LIM/homeobox, seem to be good candidates for sex-determining genes, and will need further …
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An antimicrobial peptide QUB-1925 from scorpion venom of Androctonus bicolor: molecular cloning of biosynthetic precursor-encoding cDNA and bioactivity study
… QUB-1925 precursor-encoding cDNA was cloned by “shotgun” cloning of the venom-derived cDNA library, and the peptide was chemically synthesised by using the solid phase peptide synthesiser. Reverse phase high performance liquid chromatography (RP-HPLC) and LCQ Fleet ion-trap mass spectrometer were …
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Molecular cloning and bioactivity assessment of a bioactive peptide QUB-1509 from the skin secretion of the Asian frog Hylarana guentheri
… the isolated mRNA from the skin secretion and ‘shotgun’ cloning was employed as an interrogation technique. After the mature sequence of the peptide was deduced from cloned precursor-encoding cDNA, solid phase peptide synthesis (SPPS) was employed to obtain the crude peptide. Reversed-Phase High …
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Molecular cloning, peptide synthesis and bioactive assessment of a skin defensive peptide, QUB-3117 from the skin secretion of Rana schmackeri
… the skin secretion of the Rana schmackeri by “Shotgun” Cloning. The mature peptide contains 29 amino acid residues (QUB-3117: DYMDTAKNAAKNMAAILLDKLKCKMTGGC). The functional group is carboxyl group at C-terminus. The peptide was temporarily named as QUB-3117 and it was speculated as the member …
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Identification, molecular cloning and modification of skin-derived bioactive peptides from selected Hylidae and Ranidae
… baltea and Hylarana guentheri, through ‘shotgun’ cloning. In parallel to this strategy, predicted mature peptides were identified and isolated from skin secretion using HPLC and structures verified by LC-MS. The peptides were then chemically-synthesised to produce the quantities required …
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The discovery identification and characteristion of antimicrobial peptides from amphibian frogs and scorpion venom
… Hylidae frog, Phyllomedusaduellmani, using the ‘shotgun’ molecular cloning strategy and mass spectrometry. PSPDu exhibited a broad-spectrum antimicrobial activity against seven selected microorganisms, subsequently, based on the previous study ofChapter3, PSPDu was employed as a template to …
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Exploring the Pharmacological Potential of Bioactive Peptides in Amphibians and Plants: from Identification to Rational Design
… coelestis), using the combination of ‘shotgun’ cloning and tandem mass spectrometry fragmentation sequencing. Besides, the successful molecular cloning of a novel trypsin inhibitor (as the probable alleleic variants TKTI-2 and TKTI-3) from the dried root of Trichosanthes kirilowii …
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A bioactive peptide QUB2870 from the defensive skin secretion of the Heilongjiang brown frog (Rana amurensis)
… the Heilongjiang brown frog (Rana amurensis). A “shotgun” cloning technique was employed to select the precursor sequence and a cDNA library was established for amplification of the target DNA portion. The peptide was isolated and identified using molecular cloning, online BLAST analysis, and …
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Identification and functional evaluation of a Temporin peptide, QUB-1549, from amphibian skin secretion
… from the Hylarana latouchii was subject to “shotgun cloning”. The cDNAs were amplified using a polymerase chain reaction (PCR), and subject to agarose gel analysis. The products were purified and subject to cloning (ligation, transformation, isolation of recombinant plasmid DNA and PCR). Gel …
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Bioactivity assessment of peptide QUB1471 from the skin secretion of the Chinese torrent frog (Amolops wuyiensis)
… were performed through the genomic approach shotgun cloning. The mRNA was isolated from the skin secretion and was used to construct cDNA library by reverse transcription. The target cDNA of interest was amplified and sequenced using molecular cloning. Once the sequence was confirmed, the …
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QUB-1331: a bioactive peptide from the skin secretion of the Chinese piebald odorous frog (Odorrana schmackeri)
… through the application of the molecular cloning method of ‘shotgun’ cloning and sequencing. The mature peptide was subsequently synthesised by solid phase peptide synthesis (SPPS) and its authenticity was confirmed using RP-HPLC and MALDI-TOF mass spectrometry. Later, several biological …
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QUB-3005, a bioactive peptide from the defensive skin secretion of Rana amurensis
… peptide, named QUB-3005, was isolated using “shotgun” cloning of its biosynthetic precursor-encoding cDNA from a skin secretion-derived cDNA library of Rana amurensis. The primary structure of this peptide was confirmed based on experiments of cloning and online BLAST alignment analysis. Then, …
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A bioactive peptide from the skin of the Chinese Torrent Frog, <i>Amolops wuyiensis</i>
… torrent frog (Amolops wuyiensis) by using ‘shotgun’ cloning and its encoded mature peptide, sequenced as AVPLIYNRPGVYVTKRPK-NH2, was synthesised by Fmoc-solid phase peptide synthesis. The structure of the synthetic peptide was confirmed by matrix-assisted laser desorption/ionisation …
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Identification and activity assessment of a bioactive peptide, QUB-1938, from the skin secretion of the Broad-folded frog (Hylarana latouchii)
… steps of which are as follows:1) Molecular cloning begins with the extraction of mRNA from skin secretions of target frog and the construction of its cDNA library. The full-length cDNA-encoded precursors were obtained through ‘shotgun’ cloning, and then amino acid sequences of peptide was …
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Bioactive evaluations of novel peptide QUB-2552 from the skin secretion of the Chinese Large Odorous frog, Rana (Odorrana) livida
… frog, Rana (Odorrana) livida, by applying ‘Shotgun’ cloning method. The novel peptide was synthesised by using Solid Phase Peptide Synthesis strategy. After the synthetic peptide was identified and purified by Matrix-assisted, laser desorption, ionisation, time-offlight (MALDI-TOF) and …
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Identification, isolation and bioactivity assessment of a novel peptide from the skin of Heilongjiang Brown Frog (Rana amurensis)
… peptide named QUB-2207 was identified through “shotgun” cloning of biosynthetic precursor-encoding cDNA from the skin secretion of the Heilongjiang Brown Frog, Rana amurensis. The peptide was chemically-synthesised by solid phase peptide synthesis. Antimicrobial assays and haemolytic assays were …
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The discovery of a novel bioactive peptide, QUB-1036, from the defensive skin secretion of Pelophylax kl. esculentus
… frog, Pelophylax kl. esculentus, by both a ‘shotgun’ cloning genomic study and a peptidomic study. After chemical synthesis, QUB-1036 was subjected to bioactivity tests. As a result, QUB-1036 was observed to have no inhibition against microbial growth and was completely devoid of haemolytic …
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A bioactive peptide QUB3009 from the defensive skin secretion of the frog, Phyllomedusa tomopterna
… has been isolated and identified by using “shotgun” molecular cloning of biosynthetic precursor-encoding cDNA and MALDI-TOF MS. Additionally, solid phase peptide synthesis was used for synthesis of the peptide. The synthetic replicate was subjected to functional assays, including …
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