The Development and Validation of a Quantitative Mass Spectrometric Method for the Analysis of Polypeptides in Human Plasma

An assay for the quantitative analysis of the nonapeptide arginine-8-vasopressin has been developed. This utilised sample preparation techniques such as solid phase extraction in conjunction with ultra-high performance liquid chromatography. Methods to improve the limit of detection of AVP have been performed, utilising mass spectrometry. High field asymmetric ion mobility has been trialed to assess if the interface gave benefits. The limit of detection could not reach that of immunoassay techniques of 1 pg/mL but has been validated from 0.25-10 ng/mL.

Name dc:type.qualificationname

M.Sc by research

Level dc:type.qualificationlevel

masters

Grantor dc:publisher.institution

University of York

Year dc:date.issued

2010

Author dc:creator

• Vincent, Julie C

Advisors dc:contributor.advisor

• Thomas-Oates, Jane
• Turpin, Phillip