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Virginia Tech

Efficacy of Ultraviolet Treatments for the Inhibition of Pathogens on the Surface of Fresh Fruits and Vegetables

Abstract

dc:description.abstract

Two studies investigating the use ultraviolet light at a wavelength of 253.7nm (UVC) into the inhibition of Salmonella spp. and Escherichia coli O157:H7 were conducted. The objectives of these studies were: to determine the rates for the destruction of Salmonella and Escherichia coli O157:H7 on the surface of agar and to investigate its effectiveness on the surface of fresh produce. Multiple replications of different doses and cocktail concentrations were performed and resulted in a 5 log reduction of Escherichia coli O157:H7 at doses exceeding 8.4 mW / cm2, while a 5 log reduction for Salmonella spp. was observed at doses exceeding 14.5 mW / cm². Samples of Red Delicious apples, green leaf lettuce and tomatoes were subjected to different doses ranging from 1.5 __ 24 mW / cm2 of UVC to determine effective log reductions of microbial populations. UVC applied to apples inoculated with E. coli O157:H7 resulted in the highest log reductions of approximately 3.3 logs at 24 mW/cm2. Lower log reductions (2.19 logs) were seen on tomatoes inoculated with Salmonella spp. and leaf lettuce (2.65 and 2.79) inoculated with both Salmonella spp. and E. coli O157:H7 respectfully. Due to the low capital involved in initiating a UVC system, the use of ultraviolet energy may prove to be a beneficial mechanism to decrease pathogens on fresh produce if used in conjunction with strict adherence to a sanitation program, Good Manufacturing Practices and Good Agricultural Practices in ensuring the safety of fresh produce.

Degree

thesis:*
Name thesis:degree_name
Master of Science
Level thesis:degree_level
masters
Discipline thesis:degree_discipline
Food Science and Technology
Department dc:contributor.department
Food Science and Technology
Grantor dc:publisher
Virginia Tech
Year dc:date.issued
2002

Author and committee

dc:creator, dc:contributor.*
Author dc:creator
  • Yaun, Brian Robert
Chair dc:contributor.committeechair
  • Sumner, Susan S.
Committee members dc:contributor.committeemember
  • Marcy, Joseph E.
  • Eifert, Joseph D.

Subjects

dc:subject × 3

Rights

dc:rights
Statement dc:rights
  • In Copyright

Identifiers

dc:identifier.*
Dc Identifier Other
etd-06182002-123842
OAI identifier oai:identifier
oai:vtechworks.lib.vt.edu:10919/9696

Chain of custody

source
Harvested from
Virginia Tech
Base URL
vtechworks.lib.vt.edu/oai/request
Last updated
2026-07-22
Source record
OAI-PMH GetRecord
citation

Yaun, Brian Robert. Efficacy of Ultraviolet Treatments for the Inhibition of Pathogens on the Surface of Fresh Fruits and Vegetables. masters thesis, Virginia Tech, 2002. http://hdl.handle.net/10919/9696