Abstract
dc:description.abstractGold nanoclusters possess both theoretical and practical importance in the development of ultrasensitive biosensors based on surface-enhanced Raman spectroscopy (SERS). Manipulation of gold nanoclusters in a predictable and reproducible manner for the application of refined biochemical analysis still remains challenging. In this study, high-purity gold nanoclusters are isolated via a simple method based on density gradient centrifugation. Three distinct bands including monomers, small aggregates (2-4 nanospheres), and large aggregates (>5 nanospheres) can be separated via density gradient centrifugation. The isolated gold nanoclusters greatly enhance the Raman intensity of the trapped dye molecules such that single nanocluster detection is feasible. To develop a gold nanoparticle-based biosensor for influenza virus, effort was also made to modify recognition moieties such as aptamers to gold nanoparticles via distinct approaches. The increase of hydraulic diameter and the shift of optical absorbance spectrum indicate the success of surface modification to gold nanoparticles.
Degree
thesis:*- Name thesis:degree_name
- Master of Science
- Level thesis:degree_level
- masters
- Discipline thesis:degree_discipline
- Environmental Engineering
- Department dc:contributor.department
- Civil and Environmental Engineering
- Grantor dc:publisher
- Virginia Tech
- Year dc:date.issued
- 2015
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Zhou, Xinzhe
- Chair dc:contributor.committeechair
-
- Marr, Linsey C.
- Committee members dc:contributor.committeemember
-
- Pruden, Amy
- Vikesland, Peter J.
Subjects
dc:subject × 6Rights
dc:rights- Statement dc:rights
-
- In Copyright
- Licence dc:rights.uri
Identifiers
dc:identifier.*- Dc Identifier Other
- vt_gsexam:6307
- OAI identifier oai:identifier
- oai:vtechworks.lib.vt.edu:10919/76678