Microbore HPLC methodology and temperature programmed microbore HPLC

Small diameter LC columns provide rapid thermal equilibration and are ideal candidates for temperature programmed LC. Special instrumentation requirements are presented and details of column assembly are given to permit the preparation of highly efficient, stable microbore columns. Three LC temperature control systems are described and their individual strengths and weaknesses are discussed. Problems encountered in raising the temperature of an LC column are addressed and solutions are described. Experimental results of column and instrumentation evaluation are given and the effects of temperature on speed, efficiency, stability and retention of a broad range of samples is reported. Temperature and solvent programming are compared directly.

Name thesis:degree_name

Ph. D.

Level thesis:degree_level

doctoral

Discipline thesis:degree_discipline

Chemistry

Department dc:contributor.department

Chemistry

Grantor dc:publisher

Virginia Polytechnic Institute and State University

Year dc:date.issued

1984

Author dc:creator

• Bowermaster, Jeffrey

Chair dc:contributor.committeechair

• McNair, Harold M.

Committee members dc:contributor.committeemember

• Dillard, John G.
• Field, Paul E.
• Mason, John G.
• Taylor, Larry T.

Statement dc:rights

• In Copyright

Licence dc:rights.uri

• http://rightsstatements.org/vocab/InC/1.0/

Language dc:language.iso

en_US

Handle dc:identifier.uri

http://hdl.handle.net/10919/76059

OAI identifier oai:identifier

oai:vtechworks.lib.vt.edu:10919/76059