Invitro protein synthesis and degradation and cathepsin D activity in the muscles of selenium-vitamin E deficient sheep

Invitro synthesis and degradation capabilities and the cathepsin D activity of the skeletal muscles of experimentally induced Selenium-Vitamin E deficient lambs were investigated. Twenty-one Dorset x Coopworth x Suffolk lambs were blocked by weight and assigned to one of the following treatments: 1) a basal diet of 85% whole shelled corn and 15% mixed grass hay plus a mineral supplement that was low in Se and E (-Se-E), 2) the basal diet and deficient mineral mixture as fed in treatment 1 with the addition of a casein encapsulated fat supplement (-Se-E+fat), 3) the basal diet plus a mineral mixture which contained supplemental Se and E (+Se+E). Upon completion of the feeding trial, lambs which had been receiving the -Se-E treatment had the greatest body and individual muscle weights. The -Se-E+fat treatment had the lowest degradation rates and the highest synthesis rates. Cathepsin D activity was found to be lowest in the -Se-E+fat treatment. The -Se-E treatment was observed to have the highest degradation rate and the next highest synthesis rate indicating an increased rate of muscle turn-over. In all cases, the degradation rates were positively correlated with cathepsin D activity.

Name thesis:degree_name

Master of Science

Level thesis:degree_level

masters

Discipline thesis:degree_discipline

Animal Science

Department dc:contributor.department

Animal Science

Grantor dc:publisher

Virginia Tech

Year dc:date.issued

1985

Author dc:creator

• Gore, Mitchell Taylor

Statement dc:rights

• In Copyright

Licence dc:rights.uri

• http://rightsstatements.org/vocab/InC/1.0/

Dc Identifier Other

etd-11152013-040323

OAI identifier oai:identifier

oai:vtechworks.lib.vt.edu:10919/45729